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The map label for this gene is gpsA1

Identifier: 148660333

GI number: 148660333

Start: 656234

End: 657259

Strand: Reverse

Name: gpsA1

Synonym: MRA_0571

Alternate gene names: 148660333

Gene position: 657259-656234 (Counterclockwise)

Preceding gene: 148660334

Following gene: 148660330

Centisome position: 14.87

GC content: 65.69

Gene sequence:

>1026_bases
ATGGCAGCCAACAAGCGCGAACCCAAAGTCGTTGTCCTCGGCGGCGGTTCCTGGGGCACCACCGTCGCCTCCATCTGTGC
GCGCCGCGGACCAACCTTGCAGTGGGTGCGCTCGGCGGTCACCGCGCAGGACATCAACGACAACCACCGCAATAGCCGAT
ACCTCGGCAACGACGTGGTGCTCAGCGACACCCTGCGCGCCACCACAGACTTCACCGAGGCCGCCAACTGCGCCGACGTC
GTCGTCATGGGGGTGCCCTCGCACGGTTTCCGCGGAGTGCTTGTCGAGCTGAGCAAAGAGCTGCGACCCTGGGTGCCAGT
GGTGTCCCTGGTCAAGGGGCTCGAACAGGGCACCAATATGCGGATGTCGCAGATCATCGAGGAGGTACTGCCCGGTCATC
CGGCAGGCATCCTGGCCGGCCCGAACATCGCCCGCGAGGTGGCCGAGGGCTACGCGGCCGCGGCGGTGCTGGCCATGCCC
GACCAGCACCTGGCGACCCGGCTATCAGCAATGTTTCGCACCCGGCGCTTCCGCGTGTACACAACTGACGACGTCGTCGG
TGTCGAGACGGCGGGCGCCCTCAAGAACGTCTTCGCCATCGCGGTCGGGATGGGCTATTCGCTGGGTATCGGCGAAAACA
CCCGCGCCCTGGTGATCGCCCGCGCGCTGCGCGAGATGACGAAGCTGGGTGTGGCAATGGGTGGGAAAAGCGAAACGTTC
CCCGGACTGGCCGGTCTCGGCGACCTGATCGTCACCTGCACCAGCCAACGCAGCCGCAACCGGCATGTGGGCGAACAACT
CGGCGCAGGCAAGCCGATCGACGAAATCATCGCATCGATGAGCCAGGTCGCCGAGGGCGTCAAAGCTGCCGGTGTGGTGA
TGGAGTTCGCCAACGAGTTCGGGCTGAACATGCCGATCGCCCGCGAGGTCGACGCCGTCATCAACCATGGTTCGACGGTG
GAGCAGGCGTATAGAGGCTTGATCGCCGAGGTCCCCGGACACGAGGTGCACGGCTCGGGGTTTTAG

Upstream 100 bases:

>100_bases
CGACCGCATCGAGGAGGCAATGGTGTTCGGCGCGGCATGATTGCTCCTGCGGTTCGTCGACGGATATCAAATGTCCGGTT
GCCGCAATACGCTGAACGCC

Downstream 100 bases:

>100_bases
GCTTGCCACCGCTGCCGGCACGCATGCCGGTGATGTCCGCACAACGATAGTTCGGCTGTTGCGTTCGGTCAGTGGGACGG
GCGCGTCCTCGCCGCACGCC

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase 2

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAANKREPKVVVLGGGSWGTTVASICARRGPTLQWVRSAVTAQDINDNHRNSRYLGNDVVLSDTLRATTDFTEAANCADV
VVMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNMRMSQIIEEVLPGHPAGILAGPNIAREVAEGYAAAAVLAMP
DQHLATRLSAMFRTRRFRVYTTDDVVGVETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETF
PGLAGLGDLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEFGLNMPIAREVDAVINHGSTV
EQAYRGLIAEVPGHEVHGSGF

Sequences:

>Translated_341_residues
MAANKREPKVVVLGGGSWGTTVASICARRGPTLQWVRSAVTAQDINDNHRNSRYLGNDVVLSDTLRATTDFTEAANCADV
VVMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNMRMSQIIEEVLPGHPAGILAGPNIAREVAEGYAAAAVLAMP
DQHLATRLSAMFRTRRFRVYTTDDVVGVETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETF
PGLAGLGDLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEFGLNMPIAREVDAVINHGSTV
EQAYRGLIAEVPGHEVHGSGF
>Mature_340_residues
AANKREPKVVVLGGGSWGTTVASICARRGPTLQWVRSAVTAQDINDNHRNSRYLGNDVVLSDTLRATTDFTEAANCADVV
VMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNMRMSQIIEEVLPGHPAGILAGPNIAREVAEGYAAAAVLAMPD
QHLATRLSAMFRTRRFRVYTTDDVVGVETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETFP
GLAGLGDLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEFGLNMPIAREVDAVINHGSTVE
QAYRGLIAEVPGHEVHGSGF

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=287, Percent_Identity=32.7526132404181, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI24307999, Length=349, Percent_Identity=28.3667621776504, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1790037, Length=328, Percent_Identity=36.280487804878, Blast_Score=197, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI32564399, Length=318, Percent_Identity=31.4465408805031, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17507425, Length=348, Percent_Identity=29.0229885057471, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193210136, Length=327, Percent_Identity=30.8868501529052, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564403, Length=327, Percent_Identity=30.8868501529052, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193210134, Length=315, Percent_Identity=27.6190476190476, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324513, Length=284, Percent_Identity=30.9859154929577, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320181, Length=287, Percent_Identity=30.6620209059233, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI22026922, Length=290, Percent_Identity=29.3103448275862, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136204, Length=290, Percent_Identity=31.3793103448276, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136202, Length=290, Percent_Identity=31.3793103448276, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136200, Length=290, Percent_Identity=31.3793103448276, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281362270, Length=292, Percent_Identity=26.3698630136986, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45551945, Length=292, Percent_Identity=26.3698630136986, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648969, Length=253, Percent_Identity=28.0632411067194, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA2_MYCBO (P64189)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854239.1
- ProteinModelPortal:   P64189
- SMR:   P64189
- EnsemblBacteria:   EBMYCT00000014427
- GeneID:   1091661
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0579c
- GeneTree:   EBGT00050000016691
- HOGENOM:   HBG586392
- OMA:   ASVIMEF
- ProtClustDB:   PRK12439
- BioCyc:   MBOV233413:MB0579C-MONOMER
- BRENDA:   1.1.1.94
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 36154; Mature: 36023

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 195-195 BINDING 112-112 BINDING 112-112 BINDING 144-144 BINDING 259-259 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANKREPKVVVLGGGSWGTTVASICARRGPTLQWVRSAVTAQDINDNHRNSRYLGNDVV
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
LSDTLRATTDFTEAANCADVVVMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNM
EECHHHHHHCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
RMSQIIEEVLPGHPAGILAGPNIAREVAEGYAAAAVLAMPDQHLATRLSAMFRTRRFRVY
HHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCEEEE
TTDDVVGVETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETF
ECCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
PGLAGLGDLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEF
CCHHHHHHHHHEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLNMPIAREVDAVINHGSTVEQAYRGLIAEVPGHEVHGSGF
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AANKREPKVVVLGGGSWGTTVASICARRGPTLQWVRSAVTAQDINDNHRNSRYLGNDVV
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
LSDTLRATTDFTEAANCADVVVMGVPSHGFRGVLVELSKELRPWVPVVSLVKGLEQGTNM
EECHHHHHHCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
RMSQIIEEVLPGHPAGILAGPNIAREVAEGYAAAAVLAMPDQHLATRLSAMFRTRRFRVY
HHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCEEEE
TTDDVVGVETAGALKNVFAIAVGMGYSLGIGENTRALVIARALREMTKLGVAMGGKSETF
ECCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
PGLAGLGDLIVTCTSQRSRNRHVGEQLGAGKPIDEIIASMSQVAEGVKAAGVVMEFANEF
CCHHHHHHHHHEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLNMPIAREVDAVINHGSTVEQAYRGLIAEVPGHEVHGSGF
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972