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The map label for this gene is sdhA [C]

Identifier: 148660013

GI number: 148660013

Start: 300225

End: 302165

Strand: Reverse

Name: sdhA [C]

Synonym: MRA_0257

Alternate gene names: 148660013

Gene position: 302165-300225 (Counterclockwise)

Preceding gene: 148660014

Following gene: 148660012

Centisome position: 6.84

GC content: 63.83

Gene sequence:

>1941_bases
ATGGTTGAGGTCGAGCGGCACTCCTACGACGTAGTCGTGATCGGTGCCGGCGGCGCGGGGTTACGCGCGGTCATCGAGGC
GCGGGAACGCGGCTTGAAAGTAGCCGTCGTGTGCAAATCCCTATTCGGCAAGGCCCACACGGTCATGGCCGAAGGCGGCT
GCGCGGCCGCGATGGGCAACGCCAACCCGAAGGACAATTGGAAGACTCACTTCGGCGACACGATGCGCGGTGGAAAGTTC
CTGAACAACTGGCGTATGGCCGAGCTGCACGCCAAGGAGGCGCCGGACCGGGTCTGGGAGTTGGAGACCTACGGCGCGCT
GTTCGACCGCACCGACGATGGCCGAATCAGTCAGCGCAACTTCGGCGGACACACCTATCCGCGACTGGCGCACGTCGGCG
ACCGCACCGGCCTGGAGTTGATCCGCACCCTGCAGCAAAAGGTTGTCTCGCTGCAGCAGGAGGACCACGCCGAGCTCGGC
GACTACGAAGCGCGGATCAAAGTGTTCGCCGAATGCACCATCACCGAACTGCTCAAGGACCAAGGCGCGATCGCCGGAGC
CTTCGGCTACTGGCGGGAAAGCGGCCGGTTCATCGTGTTCGAGGCGCCAGCGGTGGTGCTTGCCACTGGCGGGATCGGCA
AGTCGTTCAAGGTGACATCCAACTCCTGGGAGTACACCGGAGACGGGCACGCGTTGGCACTGCGGGCCGGGGCGACGCTG
ATCAACATGGAGTTCGTCCAGTTCCACCCGACGGGCATGGTGTGGCCGCCCAGTGTCAAGGGAATTCTGGTCACTGAGGG
TGTTCGCGGCGACGGCGGAGTGCTCAAAAACTCGGAGAACTCTCGCTTCATGTTCGACTACATTCCGCCGGTGTTCAAGG
GCCAGTACGCCGAGACGGAGGAAGAGGCCGACCAGTGGCTTAAGGACAACGACTCGGCCCGACGCACCCCGGACTTGCTG
CCCCGCGACGAGGTCGCGCGCGCGATCAACTCAGAGGTCAAGGCCGGCCGCGGTACCCCGCACGGCGGCGTCTACCTCGA
CATCGCATCCCGGCTGACACCCGCCGAGATCAAACGGCGATTGCCGTCGATGTATCACCAGTTCAAAGAGCTCGCTGAAG
TCGATATCACAACGCAGGCAATGGAAGTCGGGCCGACGTGTCACTACGTGATGGGCGGTGTCGAAGTAGACGCGGACACC
GGCGCCGCCACGGTGCCAGGCCTGTTCGCTGCCGGTGAGTGTGCCGGCGGTATGCACGGCTCCAACCGGCTGGGCGGCAA
TTCGCTGTCCGATCTGCTGGTATTCGGCCGGCGGGCCGGCCTGGGCGCAGCGGATTACGTACGGGCACTGAGCAGCCGGC
CAGCGGTTTCAGCGGAGGCTATCGATGCGGCGGCCCAGCAGGCGCTGAGCCCCTTCGAAGGACCGAAAGACGGCTCGGCG
CCGGAGAATCCGTACGCGCTGCATATGGACTTGCAGTACGTGATGAACGATCTGGTCGGTATCATCCGCAACGCCGACGA
AATATCCAGGGCGCTGACTCTTTTGGCCGAGCTGTGGTCGCGCTACCACAACGTACTCGTCGAAGGTCACCGTCAATACA
ATCCGGGCTGGAACCTGTCCATCGACCTGCGCAACATGTTGCTGGTCAGCGAGTGCGTGGCCAGAGCTGCGCTACAGCGC
ACCGAAAGCCGAGGCGGCCACACCCGAGACGACCATCCCGGTATGGACCCCAACTGGCGCAGGATCTTGCTGGTATGCCG
GGCCACCGAAACCATGGGCACCGGTGGCAGCGGGTCCGGCGACTCCAACTGCCACATCAACGTCACCCAGCAGCTGCAAA
CGCCCATGCGACCCGACTTGCTGGAGCTCTTCGAGATCTCGGAGTTGGAGAAATACTACACCGACGAAGAGCTGGCCGAG
CATCCAGGACGGAGAGGCTAA

Upstream 100 bases:

>100_bases
GGCGCTCACCGATTTCTACATCATGCTGGTGGCCAGTGGCAGCATCACAGATCTCAGATTTATTGGCTGAAGGATCGTTT
CAGAAGCTGAGCGAGGTTTT

Downstream 100 bases:

>100_bases
ATGACGTACAGCGCGAGTATGCGGGTGTGGCGCGGAGACGAAAGCTGTGGCGAACTCCGCGAATTCACCGTGGAGGTCAA
CGAGGGCGAGGTCGTGCTGG

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MVEVERHSYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAAAMGNANPKDNWKTHFGDTMRGGKF
LNNWRMAELHAKEAPDRVWELETYGALFDRTDDGRISQRNFGGHTYPRLAHVGDRTGLELIRTLQQKVVSLQQEDHAELG
DYEARIKVFAECTITELLKDQGAIAGAFGYWRESGRFIVFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGATL
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSENSRFMFDYIPPVFKGQYAETEEEADQWLKDNDSARRTPDLL
PRDEVARAINSEVKAGRGTPHGGVYLDIASRLTPAEIKRRLPSMYHQFKELAEVDITTQAMEVGPTCHYVMGGVEVDADT
GAATVPGLFAAGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPAVSAEAIDAAAQQALSPFEGPKDGSA
PENPYALHMDLQYVMNDLVGIIRNADEISRALTLLAELWSRYHNVLVEGHRQYNPGWNLSIDLRNMLLVSECVARAALQR
TESRGGHTRDDHPGMDPNWRRILLVCRATETMGTGGSGSGDSNCHINVTQQLQTPMRPDLLELFEISELEKYYTDEELAE
HPGRRG

Sequences:

>Translated_646_residues
MVEVERHSYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAAAMGNANPKDNWKTHFGDTMRGGKF
LNNWRMAELHAKEAPDRVWELETYGALFDRTDDGRISQRNFGGHTYPRLAHVGDRTGLELIRTLQQKVVSLQQEDHAELG
DYEARIKVFAECTITELLKDQGAIAGAFGYWRESGRFIVFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGATL
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSENSRFMFDYIPPVFKGQYAETEEEADQWLKDNDSARRTPDLL
PRDEVARAINSEVKAGRGTPHGGVYLDIASRLTPAEIKRRLPSMYHQFKELAEVDITTQAMEVGPTCHYVMGGVEVDADT
GAATVPGLFAAGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPAVSAEAIDAAAQQALSPFEGPKDGSA
PENPYALHMDLQYVMNDLVGIIRNADEISRALTLLAELWSRYHNVLVEGHRQYNPGWNLSIDLRNMLLVSECVARAALQR
TESRGGHTRDDHPGMDPNWRRILLVCRATETMGTGGSGSGDSNCHINVTQQLQTPMRPDLLELFEISELEKYYTDEELAE
HPGRRG
>Mature_646_residues
MVEVERHSYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAAAMGNANPKDNWKTHFGDTMRGGKF
LNNWRMAELHAKEAPDRVWELETYGALFDRTDDGRISQRNFGGHTYPRLAHVGDRTGLELIRTLQQKVVSLQQEDHAELG
DYEARIKVFAECTITELLKDQGAIAGAFGYWRESGRFIVFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGATL
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSENSRFMFDYIPPVFKGQYAETEEEADQWLKDNDSARRTPDLL
PRDEVARAINSEVKAGRGTPHGGVYLDIASRLTPAEIKRRLPSMYHQFKELAEVDITTQAMEVGPTCHYVMGGVEVDADT
GAATVPGLFAAGECAGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPAVSAEAIDAAAQQALSPFEGPKDGSA
PENPYALHMDLQYVMNDLVGIIRNADEISRALTLLAELWSRYHNVLVEGHRQYNPGWNLSIDLRNMLLVSECVARAALQR
TESRGGHTRDDHPGMDPNWRRILLVCRATETMGTGGSGSGDSNCHINVTQQLQTPMRPDLLELFEISELEKYYTDEELAE
HPGRRG

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=569, Percent_Identity=31.6344463971881, Blast_Score=251, Evalue=2e-66,
Organism=Escherichia coli, GI1786942, Length=609, Percent_Identity=35.1395730706076, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1790597, Length=546, Percent_Identity=34.4322344322344, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI1788928, Length=590, Percent_Identity=31.0169491525424, Blast_Score=213, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17505833, Length=590, Percent_Identity=34.9152542372881, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17550100, Length=591, Percent_Identity=35.7021996615905, Blast_Score=275, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71986328, Length=434, Percent_Identity=28.110599078341, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322416, Length=601, Percent_Identity=34.9417637271215, Blast_Score=293, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6322701, Length=602, Percent_Identity=34.21926910299, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6322511, Length=479, Percent_Identity=25.678496868476, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320788, Length=484, Percent_Identity=26.2396694214876, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24663005, Length=589, Percent_Identity=34.46519524618, Blast_Score=268, Evalue=1e-71,
Organism=Drosophila melanogaster, GI17137288, Length=589, Percent_Identity=32.597623089983, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24655642, Length=589, Percent_Identity=32.597623089983, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24655647, Length=589, Percent_Identity=32.597623089983, Blast_Score=261, Evalue=8e-70,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 70681; Mature: 70681

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVERHSYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAAAMGN
CCEEECCCCCEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEEECCCCEEECCC
ANPKDNWKTHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRTDDGRISQRN
CCCCCCCCHHCCCCCCCCCHHCCCEEEEHHHHCCCCHHEEHHHHHHHHCCCCCCCCCCCC
FGGHTYPRLAHVGDRTGLELIRTLQQKVVSLQQEDHAELGDYEARIKVFAECTITELLKD
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCHHEEEHEEHHHHHHHHHHH
QGAIAGAFGYWRESGRFIVFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGATL
CCCEEEHHHHHHCCCCEEEEECCEEEEEECCCCCEEEEECCCCEECCCCCEEEEECCCEE
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSENSRFMFDYIPPVFKGQYAETE
EEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCEEEEECCCHHHCCCCCCCH
EEADQWLKDNDSARRTPDLLPRDEVARAINSEVKAGRGTPHGGVYLDIASRLTPAEIKRR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHH
LPSMYHQFKELAEVDITTQAMEVGPTCHYVMGGVEVDADTGAATVPGLFAAGECAGGMHG
HHHHHHHHHHHHHHCCHHHHHHCCCCEEEEECCEEEECCCCCCCCCCHHCCCCCCCCCCC
SNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPAVSAEAIDAAAQQALSPFEGPKDGSA
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PENPYALHMDLQYVMNDLVGIIRNADEISRALTLLAELWSRYHNVLVEGHRQYNPGWNLS
CCCCEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IDLRNMLLVSECVARAALQRTESRGGHTRDDHPGMDPNWRRILLVCRATETMGTGGSGSG
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECHHHCCCCCCCCC
DSNCHINVTQQLQTPMRPDLLELFEISELEKYYTDEELAEHPGRRG
CCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure
MVEVERHSYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGKAHTVMAEGGCAAAMGN
CCEEECCCCCEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEEECCCCEEECCC
ANPKDNWKTHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELETYGALFDRTDDGRISQRN
CCCCCCCCHHCCCCCCCCCHHCCCEEEEHHHHCCCCHHEEHHHHHHHHCCCCCCCCCCCC
FGGHTYPRLAHVGDRTGLELIRTLQQKVVSLQQEDHAELGDYEARIKVFAECTITELLKD
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCHHEEEHEEHHHHHHHHHHH
QGAIAGAFGYWRESGRFIVFEAPAVVLATGGIGKSFKVTSNSWEYTGDGHALALRAGATL
CCCEEEHHHHHHCCCCEEEEECCEEEEEECCCCCEEEEECCCCEECCCCCEEEEECCCEE
INMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLKNSENSRFMFDYIPPVFKGQYAETE
EEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEECCCCCEEEEECCCHHHCCCCCCCH
EEADQWLKDNDSARRTPDLLPRDEVARAINSEVKAGRGTPHGGVYLDIASRLTPAEIKRR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHH
LPSMYHQFKELAEVDITTQAMEVGPTCHYVMGGVEVDADTGAATVPGLFAAGECAGGMHG
HHHHHHHHHHHHHHCCHHHHHHCCCCEEEEECCEEEECCCCCCCCCCHHCCCCCCCCCCC
SNRLGGNSLSDLLVFGRRAGLGAADYVRALSSRPAVSAEAIDAAAQQALSPFEGPKDGSA
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PENPYALHMDLQYVMNDLVGIIRNADEISRALTLLAELWSRYHNVLVEGHRQYNPGWNLS
CCCCEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IDLRNMLLVSECVARAALQRTESRGGHTRDDHPGMDPNWRRILLVCRATETMGTGGSGSG
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECHHHCCCCCCCCC
DSNCHINVTQQLQTPMRPDLLELFEISELEKYYTDEELAEHPGRRG
CCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]