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The map label for this gene is frdB [C]

Identifier: 148660012

GI number: 148660012

Start: 299478

End: 300224

Strand: Reverse

Name: frdB [C]

Synonym: MRA_0256

Alternate gene names: 148660012

Gene position: 300224-299478 (Counterclockwise)

Preceding gene: 148660013

Following gene: 148660009

Centisome position: 6.79

GC content: 58.63

Gene sequence:

>747_bases
ATGACGTACAGCGCGAGTATGCGGGTGTGGCGCGGAGACGAAAGCTGTGGCGAACTCCGCGAATTCACCGTGGAGGTCAA
CGAGGGCGAGGTCGTGCTGGACGTCATCCTCCGTCTACAACAGACGCAGACACCGGATCTCGCAGTGCGCTGGAATTGCA
AAGCTGGCAAGTGCGGATCCTGCTCGGCCGAGATCAACGGCAAACCGCGACTGATGTGCATGACGCGGATGTCGACATTC
GACGAGGACGAGATCGTCACGGTCACCCCAATGCGGACGTTTCCGGTGATTCGCGATCTTGTTACCGACGTCTCGTTCAA
CTACCAAAAAGCGCGCGAGATCCCGTCTTTCGCGCCACCCAAGGAGCTACAGCCCAGCGAGTACCGAATGGCGCAAGTCG
ACGTTGCACGCTCGCAGGAGTTCCGCAAGTGCATTGAATGCTTCCTGTGCCAAAACGTCTGCCACGTAGTTCGCGATCAC
GAGGAAAACAAGGATGCGTTCGCCGGCCCCCGCTTCCTGATGCGAATCGCCGAACTCGAAATGCATCCGCTGGACACCCG
GGACCGACGCAGCCAAGCACAGGAAGAGCACGGCCTGGGCTACTGCAATATCACCAAGTGCTGCACCGAAGTGTGCCCAG
AAAACATCAAGATCACCGACAATGCCCTGATCCCGATGAAAGAACGGGTCGCCGACCGCAAGTATGATCCGGTGGTATGG
CTGGGCAGCAAGCTGTTTCGCCGCTAA

Upstream 100 bases:

>100_bases
GCCCATGCGACCCGACTTGCTGGAGCTCTTCGAGATCTCGGAGTTGGAGAAATACTACACCGACGAAGAGCTGGCCGAGC
ATCCAGGACGGAGAGGCTAA

Downstream 100 bases:

>100_bases
CCCCCGGTTTTAGGTCGGGAAGTGCGCGAAACCTGAGGCAAGCCACGAACATCCACGGACTTGGCTGGAGCGAACCGCGT
CGCTGAGTAGGCAGCCGTCA

Product: fumarate reductase iron-sulfur subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTYSASMRVWRGDESCGELREFTVEVNEGEVVLDVILRLQQTQTPDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMSTF
DEDEIVTVTPMRTFPVIRDLVTDVSFNYQKAREIPSFAPPKELQPSEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDH
EENKDAFAGPRFLMRIAELEMHPLDTRDRRSQAQEEHGLGYCNITKCCTEVCPENIKITDNALIPMKERVADRKYDPVVW
LGSKLFRR

Sequences:

>Translated_248_residues
MTYSASMRVWRGDESCGELREFTVEVNEGEVVLDVILRLQQTQTPDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMSTF
DEDEIVTVTPMRTFPVIRDLVTDVSFNYQKAREIPSFAPPKELQPSEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDH
EENKDAFAGPRFLMRIAELEMHPLDTRDRRSQAQEEHGLGYCNITKCCTEVCPENIKITDNALIPMKERVADRKYDPVVW
LGSKLFRR
>Mature_247_residues
TYSASMRVWRGDESCGELREFTVEVNEGEVVLDVILRLQQTQTPDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMSTFD
EDEIVTVTPMRTFPVIRDLVTDVSFNYQKAREIPSFAPPKELQPSEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDHE
ENKDAFAGPRFLMRIAELEMHPLDTRDRRSQAQEEHGLGYCNITKCCTEVCPENIKITDNALIPMKERVADRKYDPVVWL
GSKLFRR

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG0479

COG function: function code C; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=220, Percent_Identity=25.4545454545455, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1790596, Length=202, Percent_Identity=26.2376237623762, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786943, Length=206, Percent_Identity=26.6990291262136, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533915, Length=205, Percent_Identity=25.8536585365854, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322987, Length=216, Percent_Identity=27.3148148148148, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643156, Length=232, Percent_Identity=27.1551724137931, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17137106, Length=218, Percent_Identity=24.7706422018349, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR004017
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: PF02754 CCG; PF00111 Fer2 [H]

EC number: 1.3.99.1

Molecular weight: Translated: 28626; Mature: 28495

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSASMRVWRGDESCGELREFTVEVNEGEVVLDVILRLQQTQTPDLAVRWNCKAGKCGS
CCCCCCEEEECCCCHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
CSAEINGKPRLMCMTRMSTFDEDEIVTVTPMRTFPVIRDLVTDVSFNYQKAREIPSFAPP
CCCCCCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
KELQPSEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDHEENKDAFAGPRFLMRIAELE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHH
MHPLDTRDRRSQAQEEHGLGYCNITKCCTEVCPENIKITDNALIPMKERVADRKYDPVVW
CCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCCCCEEE
LGSKLFRR
ECHHHHCC
>Mature Secondary Structure 
TYSASMRVWRGDESCGELREFTVEVNEGEVVLDVILRLQQTQTPDLAVRWNCKAGKCGS
CCCCCEEEECCCCHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
CSAEINGKPRLMCMTRMSTFDEDEIVTVTPMRTFPVIRDLVTDVSFNYQKAREIPSFAPP
CCCCCCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
KELQPSEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDHEENKDAFAGPRFLMRIAELE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHH
MHPLDTRDRRSQAQEEHGLGYCNITKCCTEVCPENIKITDNALIPMKERVADRKYDPVVW
CCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCCCCEEE
LGSKLFRR
ECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA