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The map label for this gene is murE

Identifier: 148379429

GI number: 148379429

Start: 1590297

End: 1591748

Strand: Direct

Name: murE

Synonym: CBO1457

Alternate gene names: 148379429

Gene position: 1590297-1591748 (Clockwise)

Preceding gene: 148379428

Following gene: 148379430

Centisome position: 40.91

GC content: 27.69

Gene sequence:

>1452_bases
ATGAATTTAAATTTAATACTAAAGTCATTAGAATATTCTTTTATAAAAGAAAATAAAAAGGAAATAGAAAAGATAGAATA
TGATTCAAGAAAAGTTAAAGAAGGAGATTTATTTGTTTGTATAGAGGGATATGCAACAGATGGGCATAAATATGCGAAAA
AAGCTTATGACAATGGAGCTAAAGTAATTGTTTGTGAGAAGGATTTAGAAGACTTATCCTATTATAAAGATTGTACTATA
ATAAAAGTTTCAGATAGTAGAAAAGCCTTAGCTATAATGTCATCTAATTATTATGGTAACCCATCTAAACATATTAAAAT
AATAGGTATTACAGGAACAAATGGCAAAACTACATCTACTTTTATGATGAAAGCTATACTAGAAAAAGCAGGCTATAAAG
TTGGGCTTTTGGGAACTATAGCAAATTATATAGGAAATAAAAAAATAGAATCTCATAGAACTACTCCAGAGTCTCTAGAA
CTACAAAAATTATTTAAAGATATGGTGGATGAAAAAGTAGATTATTGTGTAATGGAAGTATCTTCTCATTCTTTATATTT
AGATAGAGTTTATGGAGTAGAATTTAAAGAGGCTATATTTACAAATTTAACGCAGGATCATTTGGACTTCCATAAAACCT
TTGAAAATTATTTTAATTCCAAACTAATTTTATTTAAAAATGCTCAAAATTCAGTGATAAATATAGATGATAGTTATGGA
GAAAAAGTATTAAAGAAAGCTTTAGGAAATAAAATAACCTATGGTGTAGAGAAAAATTGTGATTTAAAAGCGGAAAATTT
ACATATGCATTCTAGAGGAGTAGAATTTGACACTATATTTAAAAATGAAAAAGAAACTATAGCTTTAAATATACCAGGTA
AATATAATATATATAATGCTTTAGGAAGTATTGGAGCTTGTCTTTTGGAGGGTATCCCTTTAAAAACTATAAAAGAAGCA
TTAGAAGATATGCCATCTGTACCAGGAAGATGTGAAATTGTAACTAAAAATTATAATTTAGGTTATGATGTTATAGTAGA
TTATGCCCATACTCCAGATGGATTAGAAAATATATTAAATACTGCTCGAGAATTTACAAAGGGAAGATTAATAAGTGTAT
ACGGATGCGGTGGAGATAGAGATAGAACTAAAAGACCAATTATGGGAAAAGTGGGGTCAAATTTAAGTGATATAGCTATA
ATAACCTCAGATAATCCAAGAACAGAGGATCCTAAACTTATAATAAAAGATGTATTAGAAGGTATAGAAAGAGATAACTA
TATAGTAGTTGAAGGAAGACGAGATGCCATAAGAAAAGCTATGGAAATAGCAAAAGAAAATGATGTTATAGTAGTGGCAG
GAAAAGGCCATGAAGATTATCAAATATTAAAGGATAAAACTATTCACTTTGATGAAAGAGAAGTAATAGAAGAACTAATA
AAAGAAATATAA

Upstream 100 bases:

>100_bases
GATATATAAATAAAAAATAAAGTTTTTTATTTACAAAAATTAAATATGGTTTTATTGTTATTATATTAAGTGACAATAAT
TAAGATAAGGAAGTGTTACA

Downstream 100 bases:

>100_bases
TTACTCTAAAAATGGGAGTGTTAACATGGAAAATATTAAACTAGAAGAAATAATAAAAGCAGTTAATGGTGAGTTAGTGA
TAACTGGAGAAAAAGATGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNLNLILKSLEYSFIKENKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGAKVIVCEKDLEDLSYYKDCTI
IKVSDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTSTFMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLE
LQKLFKDMVDEKVDYCVMEVSSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNALGSIGACLLEGIPLKTIKEA
LEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILNTAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAI
ITSDNPRTEDPKLIIKDVLEGIERDNYIVVEGRRDAIRKAMEIAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
KEI

Sequences:

>Translated_483_residues
MNLNLILKSLEYSFIKENKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGAKVIVCEKDLEDLSYYKDCTI
IKVSDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTSTFMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLE
LQKLFKDMVDEKVDYCVMEVSSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNALGSIGACLLEGIPLKTIKEA
LEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILNTAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAI
ITSDNPRTEDPKLIIKDVLEGIERDNYIVVEGRRDAIRKAMEIAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
KEI
>Mature_483_residues
MNLNLILKSLEYSFIKENKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGAKVIVCEKDLEDLSYYKDCTI
IKVSDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTSTFMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLE
LQKLFKDMVDEKVDYCVMEVSSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNALGSIGACLLEGIPLKTIKEA
LEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILNTAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAI
ITSDNPRTEDPKLIIKDVLEGIERDNYIVVEGRRDAIRKAMEIAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
KEI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=493, Percent_Identity=34.4827586206897, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1786274, Length=316, Percent_Identity=25.3164556962025, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CLOB1 (A7FTY1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383808.1
- ProteinModelPortal:   A7FTY1
- SMR:   A7FTY1
- STRING:   A7FTY1
- GeneID:   5396221
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1482
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   IGTIANY
- ProtClustDB:   PRK00139
- BioCyc:   CBOT441770:CLB_1482-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54678; Mature: 54678

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 181-181 BINDING 189-189 BINDING 380-380 BINDING 454-454 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNLILKSLEYSFIKENKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
KVIVCEKDLEDLSYYKDCTIIKVSDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTST
EEEEECCCHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCHHH
FMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLELQKLFKDMVDEKVDYCVMEV
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
SSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
CCCEEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCHHHH
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNA
HHHHHHHHCCCEEECCCCCCCCCHHCEEECCCCCEEHHEECCCCCEEEEECCCCCHHHHH
LGSIGACLLEGIPLKTIKEALEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
TAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAIITSDNPRTEDPKLIIKDVLE
HHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
GIERDNYIVVEGRRDAIRKAMEIAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
HCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHH
KEI
HHC
>Mature Secondary Structure
MNLNLILKSLEYSFIKENKKEIEKIEYDSRKVKEGDLFVCIEGYATDGHKYAKKAYDNGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
KVIVCEKDLEDLSYYKDCTIIKVSDSRKALAIMSSNYYGNPSKHIKIIGITGTNGKTTST
EEEEECCCHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCHHH
FMMKAILEKAGYKVGLLGTIANYIGNKKIESHRTTPESLELQKLFKDMVDEKVDYCVMEV
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
SSHSLYLDRVYGVEFKEAIFTNLTQDHLDFHKTFENYFNSKLILFKNAQNSVINIDDSYG
CCCEEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCHHHH
EKVLKKALGNKITYGVEKNCDLKAENLHMHSRGVEFDTIFKNEKETIALNIPGKYNIYNA
HHHHHHHHCCCEEECCCCCCCCCHHCEEECCCCCEEHHEECCCCCEEEEECCCCCHHHHH
LGSIGACLLEGIPLKTIKEALEDMPSVPGRCEIVTKNYNLGYDVIVDYAHTPDGLENILN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
TAREFTKGRLISVYGCGGDRDRTKRPIMGKVGSNLSDIAIITSDNPRTEDPKLIIKDVLE
HHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
GIERDNYIVVEGRRDAIRKAMEIAKENDVIVVAGKGHEDYQILKDKTIHFDEREVIEELI
HCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHH
KEI
HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA