| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is murF [H]
Identifier: 148379430
GI number: 148379430
Start: 1591774
End: 1593162
Strand: Direct
Name: murF [H]
Synonym: CBO1458
Alternate gene names: 148379430
Gene position: 1591774-1593162 (Clockwise)
Preceding gene: 148379429
Following gene: 148379431
Centisome position: 40.95
GC content: 28.15
Gene sequence:
>1389_bases ATGGAAAATATTAAACTAGAAGAAATAATAAAAGCAGTTAATGGTGAGTTAGTGATAACTGGAGAAAAAGATGAATATAA TTCAGTAAGCACAGATACAAGAAAAATAGAAAAGGGAGATATATTTATAGCCTTAAAAGGAGAAAATTTTAATGGAAATA ACTTTGTAGAAATGGCCATAGAAAAGGGTGCCGCTTTATGCATAGTAAGTGAAATAAATTTTAGCGAAGGAAAAGTGAGC AAATCTTCCTATGTAATAAAGGTAGAGAATACTAATAAGGCTCTTTTAGATTTAGCTAAATACTATAGAAGTAAACTAGA TATAAAAGTGGTTGCTATTACTGGATCTACAGGAAAAACATCTACAAAGGATTTAGTGGCAGCTGTGCTTTCAGAAAAAT ATAAGGTATTTAAAACAGAGGGAAATTTTAATAATGAGATAGGACTTCCTCTTATGATCTTTAAATTAGATAAAAGTTAT GATATAGCAGTTTTAGAAATGGGGATGAATCATTTTAATGAAATACACAATATGGCAGAAGCAGCAAAACCAGATATAGC TATAATAACTAATATAGGTATATCTCATATAGAAAATTTAGGATCTAGGGAAAATATTTTAAAAGCAAAACTAGAAATAA CAGATTTCTTCTGTAAGGATAGTATTTTAATAATAAATGGAGACGATGATCTTCTATCAAATTTTGAAAGTGATAAATTT AAGGTATACAAAATAGGCACAGATAAAAAATTTGATTTTAATGGAGATAATTTAAATTTAGAGGCAGAAAGTATAAAATT TGATATATTAGAACGAGGTAAAACAGCTTATAGATGTTTTAGCGTAAATGTACCTGGAAAGCATAATGTGCTTAATTCTC TTACAGCTATAGCTTGTGGAAGAGTTTTAGATATAAATTACAGGGATATACAAACTGGGATAAAAAATTTAAAAGCTACT TCTATGAGATTAGATATTGTTAAGGAAAAGGGATTTACTATAATAAATGATTGTTATAATGCAAGTCCCGATTCTATGAA AGCAGCTATAGATGTAATGAAAAATATAAAGGGAAAAAGGACTATAGCTTTATTAGGTTCTATGAGGGAACTTGGATCCG AAGGGTATAGAGCTCATAGGGAAGTTTCTGAATATGCAAGAGAAAAGGGAATAGATCTATTATTTTCTATAGGAGAATTT AGTGAAGCATATAGAGAAGGGTTTGAAGAAATAAACAAAGAGAATTATAAAAGCTTTTTAAATAATGAAGAAGCAGCAAA ATACATAAAAAATATAATAAAAGATGGTGATATAATATTAATTAAAGCATCTAGAGCTATGAAGCTAGAAGAAATAGTAA AAGAACTAAGGATAAAACAAGAAAAATAG
Upstream 100 bases:
>100_bases TATCAAATATTAAAGGATAAAACTATTCACTTTGATGAAAGAGAAGTAATAGAAGAACTAATAAAAGAAATATAATTACT CTAAAAATGGGAGTGTTAAC
Downstream 100 bases:
>100_bases GAGGTGACTTGCTCACATAATTAGAGCAATTATTTATGAATAGAATAGTATATGCAGTGCTTTTAGCCTTTTTAATATCA ATATTAGGAGGACCAATATT
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 462; Mature: 462
Protein sequence:
>462_residues MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAIEKGAALCIVSEINFSEGKVS KSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKTSTKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSY DIAVLEMGMNHFNEIHNMAEAAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF KVYKIGTDKKFDFNGDNLNLEAESIKFDILERGKTAYRCFSVNVPGKHNVLNSLTAIACGRVLDINYRDIQTGIKNLKAT SMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKRTIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEF SEAYREGFEEINKENYKSFLNNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK
Sequences:
>Translated_462_residues MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAIEKGAALCIVSEINFSEGKVS KSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKTSTKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSY DIAVLEMGMNHFNEIHNMAEAAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF KVYKIGTDKKFDFNGDNLNLEAESIKFDILERGKTAYRCFSVNVPGKHNVLNSLTAIACGRVLDINYRDIQTGIKNLKAT SMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKRTIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEF SEAYREGFEEINKENYKSFLNNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK >Mature_462_residues MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAIEKGAALCIVSEINFSEGKVS KSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKTSTKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSY DIAVLEMGMNHFNEIHNMAEAAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF KVYKIGTDKKFDFNGDNLNLEAESIKFDILERGKTAYRCFSVNVPGKHNVLNSLTAIACGRVLDINYRDIQTGIKNLKAT SMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKRTIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEF SEAYREGFEEINKENYKSFLNNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=472, Percent_Identity=31.9915254237288, Blast_Score=226, Evalue=2e-60,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 51905; Mature: 51905
Theoretical pI: Translated: 6.14; Mature: 6.14
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAI CCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEECCCEEEEEECCCCCCCCEEEEEE EKGAALCIVSEINFSEGKVSKSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKT CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCC STKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSYDIAVLEMGMNHFNEIHNMAE CHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEHHCHHHHHHHHHHHH AAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF HCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHEEEEECCCEEEEECCHHHHHCCCCCCE KVYKIGTDKKFDFNGDNLNLEAESIKFDILERGKTAYRCFSVNVPGKHNVLNSLTAIACG EEEEECCCCEECCCCCCEEEEHHHEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCC RVLDINYRDIQTGIKNLKATSMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKR CEEECCHHHHHHHHHHHHHHHEEEEEECCCCCEEEEHHCCCCCHHHHHHHHHHHCCCCCE TIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEFSEAYREGFEEINKENYKSFL EHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCHHHHHHHH NNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCC >Mature Secondary Structure MENIKLEEIIKAVNGELVITGEKDEYNSVSTDTRKIEKGDIFIALKGENFNGNNFVEMAI CCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEECCCEEEEEECCCCCCCCEEEEEE EKGAALCIVSEINFSEGKVSKSSYVIKVENTNKALLDLAKYYRSKLDIKVVAITGSTGKT CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCC STKDLVAAVLSEKYKVFKTEGNFNNEIGLPLMIFKLDKSYDIAVLEMGMNHFNEIHNMAE CHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEHHCHHHHHHHHHHHH AAKPDIAIITNIGISHIENLGSRENILKAKLEITDFFCKDSILIINGDDDLLSNFESDKF HCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHEEEEECCCEEEEECCHHHHHCCCCCCE KVYKIGTDKKFDFNGDNLNLEAESIKFDILERGKTAYRCFSVNVPGKHNVLNSLTAIACG EEEEECCCCEECCCCCCEEEEHHHEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCC RVLDINYRDIQTGIKNLKATSMRLDIVKEKGFTIINDCYNASPDSMKAAIDVMKNIKGKR CEEECCHHHHHHHHHHHHHHHEEEEEECCCCCEEEEHHCCCCCHHHHHHHHHHHCCCCCE TIALLGSMRELGSEGYRAHREVSEYAREKGIDLLFSIGEFSEAYREGFEEINKENYKSFL EHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCHHHHHHHH NNEEAAKYIKNIIKDGDIILIKASRAMKLEEIVKELRIKQEK CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]