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The map label for this gene is folD

Identifier: 148379158

GI number: 148379158

Start: 1287187

End: 1288035

Strand: Direct

Name: folD

Synonym: CBO1172

Alternate gene names: 148379158

Gene position: 1287187-1288035 (Clockwise)

Preceding gene: 148379157

Following gene: 148379159

Centisome position: 33.12

GC content: 26.97

Gene sequence:

>849_bases
ATGGCTAAAATATTATATGGAAATGAAGTAGCTTTAAAAATAAAGGAAGATTTAAATTTAAGAATAGATAAATTAAAAGA
AAAAAATATAATACCTAAGTTAGCAATTTTACGTATGGGAAATAAACCAGACGATATAGCCTATGAAAGAAGTATAATAA
AAAGCTGTGAAAAACTAAACATAGAAACTAAGGTAGAAGAATTAAATGAAGATATATTAGAAGAAGATTTCTTGAAGTTA
ATGGAAAGTTTAAATAACGAAAAGGAAATTCATGGTATATTAGTCTTTAGACCTTACCCTAAACATTTAAATGAAAATAT
AATAAACTCTTCTATAGCATTAAATAAAGATGTGGATTGCATGCATCCTTTAAATTTAGAAAGGATATTTGAAGGAGATT
TAAATGGATTTATGCCTTGTACTCCGGAGGCTGTAATAGAAATATTAAAATATTATGATATAGATTTAAAAGGAAAGAAT
ATAGTTATTATAAACAGAAGTATGGTAGTGGGCAAACCATTGAGTATGATGGTCTTATCTCATAATGCTACAGTTACTAT
ATGTCATTCAAGAACTATAGATTTGCCATCTATAACTAAAAAAGCAGATATAGTAGTGACAGCTATAGGAAAAGCTAAAT
TAATAAAAGAAGAATATTTTAATGAAGATTCTATAGTTATGGATGTAAGCATTAATATAGATGAAAATGGAAAACTATGT
GGAGATGTGGATTTTGAAAATGTAAAAGAAAAAGTAGGAGCTATAACTCCAGTTCCAAAAGGAGTAGGAAGTGTTACAAC
TACCTTGTTATTAAAACACATAGTAGATGCAGCAGAAAGAAATAGTTAA

Upstream 100 bases:

>100_bases
ATTCTTTGGAAGAAGAAATCAACACCTTAGTTTCTAAATATTCAACTAAGGCAGATAAAGTTTATAGTTATGTAGAAAAT
TTAATAAGGGGAAATGAATA

Downstream 100 bases:

>100_bases
ATATAAAAATTAAAAATTAAAGCCTGTATCCTATGTTAGTTTATATAGGATACAGGCTTATTTTTCTTATAGTAAATAAT
ATATAAGATTTTATAGATTC

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MAKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKL
MESLNNEKEIHGILVFRPYPKHLNENIINSSIALNKDVDCMHPLNLERIFEGDLNGFMPCTPEAVIEILKYYDIDLKGKN
IVIINRSMVVGKPLSMMVLSHNATVTICHSRTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINIDENGKLC
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS

Sequences:

>Translated_282_residues
MAKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKL
MESLNNEKEIHGILVFRPYPKHLNENIINSSIALNKDVDCMHPLNLERIFEGDLNGFMPCTPEAVIEILKYYDIDLKGKN
IVIINRSMVVGKPLSMMVLSHNATVTICHSRTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINIDENGKLC
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS
>Mature_281_residues
AKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKLM
ESLNNEKEIHGILVFRPYPKHLNENIINSSIALNKDVDCMHPLNLERIFEGDLNGFMPCTPEAVIEILKYYDIDLKGKNI
VIINRSMVVGKPLSMMVLSHNATVTICHSRTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINIDENGKLCG
DVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=36.518771331058, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=36.271186440678, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=34.2372881355932, Blast_Score=145, Evalue=3e-35,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=34.2960288808664, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=32.9896907216495, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=37.9790940766551, Blast_Score=204, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=269, Percent_Identity=37.546468401487, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=21.5277777777778, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=34.2560553633218, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=34.2560553633218, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=34.2560553633218, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=34.2560553633218, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=32.3232323232323, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=32.3232323232323, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CLOB1 (A7FT56)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383533.1
- ProteinModelPortal:   A7FT56
- SMR:   A7FT56
- STRING:   A7FT56
- GeneID:   5398001
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1203
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GFPPCTP
- ProtClustDB:   CLSK941298
- BioCyc:   CBOT441770:CLB_1203-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31696; Mature: 31565

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLN
CCEEEECCEEEEEEECCCCCEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
IETKVEELNEDILEEDFLKLMESLNNEKEIHGILVFRPYPKHLNENIINSSIALNKDVDC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCEEECCCCCC
MHPLNLERIFEGDLNGFMPCTPEAVIEILKYYDIDLKGKNIVIINRSMVVGKPLSMMVLS
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCEEECCCCEEEEEE
HNATVTICHSRTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINIDENGKLC
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLN
CEEEECCEEEEEEECCCCCEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
IETKVEELNEDILEEDFLKLMESLNNEKEIHGILVFRPYPKHLNENIINSSIALNKDVDC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCEEECCCCCC
MHPLNLERIFEGDLNGFMPCTPEAVIEILKYYDIDLKGKNIVIINRSMVVGKPLSMMVLS
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCEEECCCCEEEEEE
HNATVTICHSRTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINIDENGKLC
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA