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The map label for this gene is ybjI [H]

Identifier: 148379159

GI number: 148379159

Start: 1288355

End: 1289149

Strand: Direct

Name: ybjI [H]

Synonym: CBO1173

Alternate gene names: 148379159

Gene position: 1288355-1289149 (Clockwise)

Preceding gene: 148379158

Following gene: 148379161

Centisome position: 33.15

GC content: 25.03

Gene sequence:

>795_bases
ATGATAAAATTTATAGCTACAGATTTAGATGGAACACTAGTAAATAGTGAAGGTAAAATATATAATAAGGTGTTTAATTT
AATAAACGATTTACATAAAAATGGGGTAAAGTTTGCAGCAGCTAGTGGAAGATTTTATTCTCAATTAAATGAAAATTTCA
ATAGTGTAAAGGAAGATATGATACTTATAGCTCATAATGGAGCTCTTATAAAATATAGTAAAAATGGACAGACTCTTTAT
GCTAATTATATAGATAAAGAATATATAAAATCAGTAGAAAAATTAAAAAGAAATTTTGGAGAAGAATTAATTTTAGCGGG
AGAAAATGAAGCTTTTGTTGTAAATCCTTCTCAAAGTATTAAAGAAGAATTTAATTTTTATAATGTACCTTATATAGAAT
ACAAATCCTTTGATGAAGTGGATAAGCCTGTTCAAAAGATAAGTTATTATGTTAAAGATGGTATTAAAGCATCTATGATC
GATTATTTAAAAGAAAATTTAAATAAGAATCTTCAATTTGTTGCTTCAGGGGATAAATGGATAGATATGATGAATAAAGA
AGTAAGTAAAGGACATGCCATAAAAATACTTCAGAAAAAATTTAATATAGAAAAAGATAATACTATGGTTTTTGGAGATT
ATTATAATGATATAACCATGTTTAAGCAAGCTTATTATAGTTATGCTATGGAAAATGCTCCAGAAGATGTAAAAGAAAAG
GCCAATTTTATAGCTGGTAACAATAACGAAAATGCAGTTTATAAAACTATAAGTAAACATATGGGATTTATTTAA

Upstream 100 bases:

>100_bases
GTTGTGATATAATAGGAAAGTAGAAAAAACATATTTTTTAAATATAGAAATCATATGGGATTTTCAAATATAAAATTACC
ATAAAACATAGGAGGAAAAA

Downstream 100 bases:

>100_bases
TATAAAAACTGTTTTAAAATATACTTTTCCCATAGGAAATTTTACTGATAAATTTTAATAACTATAAATAAACACTATCA
AAATCTATTAGAATTGATAG

Product: HAD hydrolase, IIB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDMILIAHNGALIKYSKNGQTLY
ANYIDKEYIKSVEKLKRNFGEELILAGENEAFVVNPSQSIKEEFNFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKASMI
DYLKENLNKNLQFVASGDKWIDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFKQAYYSYAMENAPEDVKEK
ANFIAGNNNENAVYKTISKHMGFI

Sequences:

>Translated_264_residues
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDMILIAHNGALIKYSKNGQTLY
ANYIDKEYIKSVEKLKRNFGEELILAGENEAFVVNPSQSIKEEFNFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKASMI
DYLKENLNKNLQFVASGDKWIDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFKQAYYSYAMENAPEDVKEK
ANFIAGNNNENAVYKTISKHMGFI
>Mature_264_residues
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDMILIAHNGALIKYSKNGQTLY
ANYIDKEYIKSVEKLKRNFGEELILAGENEAFVVNPSQSIKEEFNFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKASMI
DYLKENLNKNLQFVASGDKWIDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFKQAYYSYAMENAPEDVKEK
ANFIAGNNNENAVYKTISKHMGFI

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=264, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787043, Length=264, Percent_Identity=29.5454545454545, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI2367265, Length=268, Percent_Identity=26.4925373134328, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30420; Mature: 30420

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDM
CCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCE
ILIAHNGALIKYSKNGQTLYANYIDKEYIKSVEKLKRNFGEELILAGENEAFVVNPSQSI
EEEEECCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHH
KEEFNFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKASMIDYLKENLNKNLQFVASGDKW
HHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHH
IDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFKQAYYSYAMENAPEDVKEK
HHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHH
ANFIAGNNNENAVYKTISKHMGFI
HHEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKFIATDLDGTLVNSEGKIYNKVFNLINDLHKNGVKFAAASGRFYSQLNENFNSVKEDM
CCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCE
ILIAHNGALIKYSKNGQTLYANYIDKEYIKSVEKLKRNFGEELILAGENEAFVVNPSQSI
EEEEECCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHH
KEEFNFYNVPYIEYKSFDEVDKPVQKISYYVKDGIKASMIDYLKENLNKNLQFVASGDKW
HHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHH
IDMMNKEVSKGHAIKILQKKFNIEKDNTMVFGDYYNDITMFKQAYYSYAMENAPEDVKEK
HHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHH
ANFIAGNNNENAVYKTISKHMGFI
HHEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]