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The map label for this gene is trpE [H]

Identifier: 148359279

GI number: 148359279

Start: 2014569

End: 2016716

Strand: Reverse

Name: trpE [H]

Synonym: LPC_1175

Alternate gene names: 148359279

Gene position: 2016716-2014569 (Counterclockwise)

Preceding gene: 148359287

Following gene: 148359278

Centisome position: 56.39

GC content: 38.22

Gene sequence:

>2148_bases
ATGTTACAACAATATAAAACTCTCGGTGGTGTTCATGTTGAGTTTTCTCAACAAGAGTTGGATTATCAACAAGGCATAGA
TTTTTTGCTTGAGAGTCTTGATTCTCAACGTGGAGCAGTATTTGCTTCGAGCTTTGAATATCCGGGACGCTATACTTGTT
GGGACATGGGTTTTTATAATCCCCCATTAGTTATGATCTGTCAAAATAATACCATATGTTTCGAGGCATTAAATCAACGG
GGCGAGATATTATTATCTTTTCTTTATCCGTTGTTAAAAACAACAGATCTCTGGGAGGTAATTAGCCAAACGACCTCATT
TTGTCAGTTAAAAATTAAATCATCAGACAAAGTATTTAGTGAGGAGGAGCGGAGCCATCAACCGTCAGTATTCAGTGCAA
TTCGCCTCTTATTGAATTTTTTTAAAACAGAAGAAGATTCATACTTGGGTTTATATGGTGCTTTTGGGTTTGACCTGATT
TATCAATTTGAACAGTTGGAGCGGTGTAAACCAAGAAATGAAACACAGAGAGATATGGTTCTCTATCTACCTGATGAAAT
TTATATAGTGAATCATCGTAAGGAAGAAGCCTTTGTTAGACGCTATGATTTTCAATATCAGGGGCGATCAACTCAATCTT
TATCCAGAGAAGGGAAGTATTCACCATATCAACCTATCCATAAACCGGAAAAAAACTGTGATCACGAGCCAGGTGAATAT
GCCGAAGTGGTCAAAAAAGCCAAAGAAAAATTTTCCTGTGGTGATTTATTTGAAGTGGTTCCTAGCCAAACTTTCTATAC
TCACTTTGCTGAAAAGCCGTCAATATTATTTCGCCAAATGCGAGAAATAAATCCATCGCCATATGGATTTTTTGTTAATT
TGGGTGACGAAGAATATCTGGTAGGTGCTTCACCTGAAATGTATGTTCGTGTCCAAGGTAAAAGAGTAGAAACTTGCCCT
ATCTCAGGCACTATCAAACGGGGAGCAGATGCTATAGAGGATGCTCACAATATTCAAACACTTCTAGATTCAGAGAAAGA
AGAATCAGAACTAACCATGTGCACCGATGTGGATAGAAATGATAAGTCAAGAATATGCGAAGCAGGGAGTGTGAAAGTCA
TTGGGCGTCGCCAAATCGAAATGTATTCCCGATTGATTCATACAGTTGATCATGTTGAGGGTATTTTAAGAGATGGATTT
GATGCAGTAGATGCTTTTTTAACTCATATGTGGGTTGTTACTGTAACTGGTGCGCCTAAAATTTGGGCTATGAATTTCAT
TGAACAACATGAGAAATCCCCACGTAAGTGGTATGCAGGGGCTGTAGGATGGTTTGGTTTTGATGGAAATTTAAATACTG
GATTGGTCTTAAGAACAGTGCGAATAGAAAAGGGAACTGCAGAAATTAGAGTAGGCGCAACGTTACTTTATGACTCTATC
CCTGAAGCTGAGGAAGAGGAGACGAGATTAAAAGCTTCTGCTTTTTTAGACATTTTACAAAAACCATGGCAAAAGGCTAA
AAAAAAGACAGAAGATGTTCCCCTGGTAGGCAAAAATAAAAAAGTTTTACTTATCGATCATCAAGATTCCTTTGTTCATA
CTTTGGCAAACTATTTAAGACAAACGGGAGCTGCGGTTACAACAGTTCGCTATGACAAGGCGATTGGCTATTTACAAAAT
AATCATTTTGATTTGATCGTATTATCGCCTGGCCCCGGAAAACCGGTTGATTTTAAGCTTTCAGAAACAATCGATGTGAT
ACTCTCAAAGAACATACCAATATTCGGTGTTTGTCTGGGGCTGCAAGGAATTGTTGAATATTTTGGAGGGGTTTTGGATG
TTCTTGAATACCCTATGCATGGAAAATCTTCAGTAATCAGGGTATTAGATAACAAGGGGTTGTTCTCCGGTTTAGGTGAT
TGTTTTAAAGCGGGAAGATACCATTCACTCTATGCCAGAGCAGAGGCTTTACCCAATGAATTATTAGTTACCGCTGTAAG
TGAAGATGATGTGATTATGGCAGTTTCTCATGAGTATTTACCTGTGCATGCGGTTCAATTTCATCCAGAGACAATTTTGT
CTTTATCGGATCAAGTGGGTTTAAGAATAATCATCAATTTGATGAACATGGTGAAGGACAATGGGTAA

Upstream 100 bases:

>100_bases
AAAAAAGCAATGATATTGAAATGTTACAAGATTCTTGCAGAACAGTGGTTTTACAGTAGAATGAATATTTGTACGAATTA
AATGATAGAGAATGGTATTC

Downstream 100 bases:

>100_bases
AAAAATACTGATTTTATATTTTGTATCAGTTCTATTAGGGATATTAACAGGCGCAATTGCTTCATTATTCCAACTTACTA
TTCAACAGATGGATCATCTG

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYNPPLVMICQNNTICFEALNQR
GEILLSFLYPLLKTTDLWEVISQTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEPGEY
AEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCP
ISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGILRDGF
DAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLYDSI
PEAEEEETRLKASAFLDILQKPWQKAKKKTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQN
NHFDLIVLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSSVIRVLDNKGLFSGLGD
CFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHEYLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG

Sequences:

>Translated_715_residues
MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYNPPLVMICQNNTICFEALNQR
GEILLSFLYPLLKTTDLWEVISQTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEPGEY
AEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCP
ISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGILRDGF
DAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLYDSI
PEAEEEETRLKASAFLDILQKPWQKAKKKTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQN
NHFDLIVLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSSVIRVLDNKGLFSGLGD
CFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHEYLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG
>Mature_715_residues
MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYNPPLVMICQNNTICFEALNQR
GEILLSFLYPLLKTTDLWEVISQTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEPGEY
AEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCP
ISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGILRDGF
DAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLYDSI
PEAEEEETRLKASAFLDILQKPWQKAKKKTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQN
NHFDLIVLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSSVIRVLDNKGLFSGLGD
CFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHEYLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=421, Percent_Identity=32.3040380047506, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788114, Length=352, Percent_Identity=27.5568181818182, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789760, Length=191, Percent_Identity=37.696335078534, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787517, Length=177, Percent_Identity=36.1581920903955, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786809, Length=304, Percent_Identity=26.6447368421053, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI87082077, Length=288, Percent_Identity=23.2638888888889, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=277, Percent_Identity=36.101083032491, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322638, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324361, Length=125, Percent_Identity=32.8, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 81371; Mature: 81371

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYN
CCCCHHHCCCEEEEECHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCC
PPLVMICQNNTICFEALNQRGEILLSFLYPLLKTTDLWEVISQTTSFCQLKIKSSDKVFS
CCEEEEECCCEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCC
EEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLIYQFEQLERCKPRNETQRDMV
HHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEPGEY
EEECCEEEEEECCHHHHHHHHHCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHH
AEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYL
HHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCEE
VGASPEMYVRVQGKRVETCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRN
ECCCCCEEEEEECCEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
DKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGILRDGFDAVDAFLTHMWVVTVTGAPK
CHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCC
IWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLYDSI
HHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCCCEEEEEEEEECCCCEEEECEEEEECCC
PEAEEEETRLKASAFLDILQKPWQKAKKKTEDVPLVGKNKKVLLIDHQDSFVHTLANYLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCHHHHHHHHHHH
QTGAAVTTVRYDKAIGYLQNNHFDLIVLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLG
HCCCEEEEEEHHHHHHHEECCCEEEEEECCCCCCCEEEEHHHHHHHHHCCCCCEEEHHHH
LQGIVEYFGGVLDVLEYPMHGKSSVIRVLDNKGLFSGLGDCFKAGRYHSLYARAEALPNE
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCE
LLVTAVSEDDVIMAVSHEYLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG
EEEEEECCCCEEEEEECCCCEEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASSFEYPGRYTCWDMGFYN
CCCCHHHCCCEEEEECHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCC
PPLVMICQNNTICFEALNQRGEILLSFLYPLLKTTDLWEVISQTTSFCQLKIKSSDKVFS
CCEEEEECCCEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCC
EEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLIYQFEQLERCKPRNETQRDMV
HHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREGKYSPYQPIHKPEKNCDHEPGEY
EEECCEEEEEECCHHHHHHHHHCCEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHH
AEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSILFRQMREINPSPYGFFVNLGDEEYL
HHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCEE
VGASPEMYVRVQGKRVETCPISGTIKRGADAIEDAHNIQTLLDSEKEESELTMCTDVDRN
ECCCCCEEEEEECCEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
DKSRICEAGSVKVIGRRQIEMYSRLIHTVDHVEGILRDGFDAVDAFLTHMWVVTVTGAPK
CHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCC
IWAMNFIEQHEKSPRKWYAGAVGWFGFDGNLNTGLVLRTVRIEKGTAEIRVGATLLYDSI
HHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCCCEEEEEEEEECCCCEEEECEEEEECCC
PEAEEEETRLKASAFLDILQKPWQKAKKKTEDVPLVGKNKKVLLIDHQDSFVHTLANYLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCHHHHHHHHHHH
QTGAAVTTVRYDKAIGYLQNNHFDLIVLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLG
HCCCEEEEEEHHHHHHHEECCCEEEEEECCCCCCCEEEEHHHHHHHHHCCCCCEEEHHHH
LQGIVEYFGGVLDVLEYPMHGKSSVIRVLDNKGLFSGLGDCFKAGRYHSLYARAEALPNE
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCE
LLVTAVSEDDVIMAVSHEYLPVHAVQFHPETILSLSDQVGLRIIINLMNMVKDNG
EEEEEECCCCEEEEEECCCCEEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]