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The map label for this gene is dotB [H]

Identifier: 148358589

GI number: 148358589

Start: 3199296

End: 3200405

Strand: Direct

Name: dotB [H]

Synonym: LPC_0461

Alternate gene names: 148358589

Gene position: 3199296-3200405 (Clockwise)

Preceding gene: 148358590

Following gene: 148358588

Centisome position: 89.45

GC content: 42.88

Gene sequence:

>1110_bases
ATGCCCGATGAGCCAACTCGTTTCACTCCAGTATTCATGGACAGAATGCTGGAGCATGCAGAAAGCCTGAATGCTTCAGA
TATAACAATCCAAACTGGCGAACCCATTTTTGCAGAGGTTTATGGAAGACTCCTTAAAATTACAAATCGACGTCTTTCCA
ATACAGAATTGGGCGACCTTATTAATTCCATTTATGGCCCGAATGCCACGACACAATTACTTTCCGGTAAAGATATAGAT
ACTCATTATGAGTTCCGACCCAACCGTGGTGTACGCTATCGATATAGGGTTAACGCGACTGCCTGCCTGGTTGAAGGTCA
TGATGCCATTCAAATCACATTAAGAACTATTCCAACCACGCCACCCAAACTCAGTACCATGAACCTCCCTGATAACATTA
TTGAAGCGATTGCTCCACAGGAAGGAATCGTGTTTATTACAGGCGCTACCGGTTCGGGCAAATCAACATTGTTAGCTTCC
ATTATTCGAGAACTTATTGAAACATCAGACTCAAACCGTAAAGTATTAACTTACGAATCCCCGATAGAATTCGTCTATGA
TGAAATTGAAACAATTTCCGCTGTAGTAAGCCAATCAGAGATTCCAAGACACTTACCCAACTTTGCTGATGGAGTAAGAA
ACGCATTAAGACGAAAGCCAAGATTAATTATGGTGGGTGAATGTCGTGATGCAGAAACCATCAGTGCCGCTCTTGAAGCC
GCTTTAACAGGGCACCCTGTCTACACAACCCTGCATACTTCTGGTGTTGCAGAAACAATGCGACGTCTGGTCACATCTTT
TAGCGGGGAAGAGCGTTTGGGAAGAACCATCGATATTCTTGAAACAATTAGGCTTTGCATTTGGCAAAAACTGGTCCCAA
CCGTTGATGAAAGACGTGTAGCCCTAAGGGAGTATCTGGTATTCGATGAAGAAGTGAGAGATATTCTATTGGAGGGAGAC
CCTAACGAAGTGACTTCAGCCACTCGTAAGTTAGTGAGGCAAAAAGGGCAGTTAATGACTTGGGACGCAAAAATGAAATT
TGAACAGGGGATTATCAGTGAGCGAGTTTATAAATTAATCATTGCTGGGGCCAAAGAATATCAACAATAA

Upstream 100 bases:

>100_bases
TGGAAAAACTGGCAAATCAGGCTAAAATTGTTATAACAAACAAATCCTGGCAACCTATTATAGCACCAGTAAGTTAATGT
CTATGGATAATATTAATTTA

Downstream 100 bases:

>100_bases
TCAAAATTAATCTGTTTGCACTTGCCATAGAAAAATCATTGATATACTACCGCATATAAAACAATCAAGCTCAAGGATTC
GATTCATGATGTTTCACAGG

Product: organelle trafficking lipoprotein defect DotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDID
THYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLAS
IIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAETISAALEA
ALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGD
PNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ

Sequences:

>Translated_369_residues
MPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDID
THYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLAS
IIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAETISAALEA
ALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGD
PNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ
>Mature_368_residues
PDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDT
HYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASI
IRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAETISAALEAA
LTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDP
NEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=216, Percent_Identity=38.4259259259259, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789723, Length=150, Percent_Identity=39.3333333333333, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786296, Length=133, Percent_Identity=39.0977443609023, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41537; Mature: 41406

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDL
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHH
INSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTT
HHHHCCCCCHHHHHCCCCCCCCEEECCCCCCEEEEEECCEEEEEECCCEEEEEEEECCCC
PPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASIIRELIETSDSNRKVLTYES
CCCCEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
PIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAETISAALEA
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
ALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRV
HHCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHH
ALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
IAGAKEYQQ
HHCHHHHCC
>Mature Secondary Structure 
PDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDL
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHH
INSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTT
HHHHCCCCCHHHHHCCCCCCCCEEECCCCCCEEEEEECCEEEEEECCCEEEEEEEECCCC
PPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASIIRELIETSDSNRKVLTYES
CCCCEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
PIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAETISAALEA
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
ALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRV
HHCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHH
ALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
IAGAKEYQQ
HHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]