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The map label for this gene is yhcR [H]

Identifier: 148358588

GI number: 148358588

Start: 3200491

End: 3202218

Strand: Direct

Name: yhcR [H]

Synonym: LPC_0460

Alternate gene names: 148358588

Gene position: 3200491-3202218 (Clockwise)

Preceding gene: 148358589

Following gene: 148358585

Centisome position: 89.49

GC content: 38.6

Gene sequence:

>1728_bases
ATGATGTTTCACAGGAAAAGCCTATATCATTATTTTAACTTATTTATTTTGTTAATTTTAGGCTCTTCTCTCAATTGGGC
TTCCGTCAATCAGCACAGCACAGCTTCGCCAACCCTCAACATTAAAATTTTAGGCATTAATGATTTTCATGGTCAAATAA
GCAGTGGGAGAGCCGTAAAAAATGAACCTGTTGGCGGGGCAGCTGTCCTGGCCGCTTATCTTAAAGAAGCTCAATTGGGA
ATGGAAGATCGATCAATAATTACCATCATGGGTGATTTGGTTGGAGCCTCCCCCCCTTCTTCAGGACTGTTGAATGATGA
ACCCAGTATTTTGTTTATTAATGGCTTGGGAAATAATCAATGCAAGCCCGAATCAAGAATGGATCCTCTTTGCAACATCG
TTGCCACAGTAGGAAACCATGAATTTGACAAAGGGCAAAAGGCCATGTTTGATTTAATTTATGGTACCAATAATCCACCG
ACCGATTCATGGATCTCATTACCCAATTATCCGGGCGCATCTTATCCCTATGTTTCTGCGAATATCGTTGACGCCAAGTC
AGAACAACCTCTCTTCCCACCCTACATAATTAAACGGATACATAATACTCCCATAGCATTTATTGGCGCTGTTTTAAAAG
ATGCGGCGGACTCCATGTTTCCAGCAAACGCAGAAGGAGTTAAATTTTTAGATGAAGCAGACAGTATTAATCATTACATC
CCGGAAATTAAAGCGAAAGGAGTCAATGTTATTATTGTCCTGATTCATGAAGGTGGAAATCAAATTCCTTATGAGGGAGA
CACCCAAACTACCTCAGCAGTAGAAGGTCATATTAAAGAAATAGTTTATTCCCTTGGTGATGAAATCGACGTTGTTATGG
GTGGTCACACCCATCAATTTCTAAATGCTTTCCTTCCAAATCGCAATGGGCATAAGATCCTGGTCACACAAGCGAATAGT
TACAGTGCTGCTTTTGCTGAAGTTACCCTCCAAATCGATACCAAAAATCATACGGTAAAAAACAAATCAGCCAAAATTAT
TACAACTTATGCTAATCGATGGCCTGGAACAAATCCGGATACACAAGCCTTGGAAATTGTTAAATTAGCAGAAGATAAAA
TTGGGCCAATAATAAATAGCCATGTAGGCACTGCAGAATATGATCTGCTAAGAAAACAAAATAAAGATGGTGAATCGAGT
TTAGGGAATCTGATCGCAGATGCTTATAGAACAGTAATGAATACCGATCTGGGTATCACTAATCCCAATGGAATTCGTGA
TGATTTAAAAGCAGGGGAAGTTAACTGGGGAAATATCTATTCTATTTTACCCTTTTCCAACAGCATAGTCACAATTACCT
TAACTGGAAATGATATTTATGACTTGTTAGAGCAACAATGGATGGGCTCTTATATCAATATGTTACAAATTTCCGGGTTT
GCCTACACCTATGATTCACATCAACCTCTGGGACATAAAATAATTGAAATTAGGCATCAAAATAAACCATTGCTTAGAGA
AAAGATGTATACCATAGCCACTTCAGATTTTCTTGCCAGCGGGAATGGAGTTTTTTCAGTTATGAAAAGAGGAAAAATAA
TCCATGTTGGTCAAAGTGATCATGACACGGTAATCGCTTATATTAAAAGTTTACCTCAACCCTTTCACGCGTCCATTGAG
GCAAGGATAAAAGAATCAGAGACTGGATTAAAAACAGGAGTTGGATAG

Upstream 100 bases:

>100_bases
GAATATCAACAATAATCAAAATTAATCTGTTTGCACTTGCCATAGAAAAATCATTGATATACTACCGCATATAAAACAAT
CAAGCTCAAGGATTCGATTC

Downstream 100 bases:

>100_bases
ACTAAATTCGTTTTACCGTCAGCCCCTGTTTTTATTATTTATTAATTAAGTTTTGCTATTATTTTATGCATTCTAATCCA
GCACATAGTAGAATTTGTTT

Product: 5'-nucleotidase

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MMFHRKSLYHYFNLFILLILGSSLNWASVNQHSTASPTLNIKILGINDFHGQISSGRAVKNEPVGGAAVLAAYLKEAQLG
MEDRSIITIMGDLVGASPPSSGLLNDEPSILFINGLGNNQCKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPP
TDSWISLPNYPGASYPYVSANIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADSMFPANAEGVKFLDEADSINHYI
PEIKAKGVNVIIVLIHEGGNQIPYEGDTQTTSAVEGHIKEIVYSLGDEIDVVMGGHTHQFLNAFLPNRNGHKILVTQANS
YSAAFAEVTLQIDTKNHTVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAEYDLLRKQNKDGESS
LGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNSIVTITLTGNDIYDLLEQQWMGSYINMLQISGF
AYTYDSHQPLGHKIIEIRHQNKPLLREKMYTIATSDFLASGNGVFSVMKRGKIIHVGQSDHDTVIAYIKSLPQPFHASIE
ARIKESETGLKTGVG

Sequences:

>Translated_575_residues
MMFHRKSLYHYFNLFILLILGSSLNWASVNQHSTASPTLNIKILGINDFHGQISSGRAVKNEPVGGAAVLAAYLKEAQLG
MEDRSIITIMGDLVGASPPSSGLLNDEPSILFINGLGNNQCKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPP
TDSWISLPNYPGASYPYVSANIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADSMFPANAEGVKFLDEADSINHYI
PEIKAKGVNVIIVLIHEGGNQIPYEGDTQTTSAVEGHIKEIVYSLGDEIDVVMGGHTHQFLNAFLPNRNGHKILVTQANS
YSAAFAEVTLQIDTKNHTVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAEYDLLRKQNKDGESS
LGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNSIVTITLTGNDIYDLLEQQWMGSYINMLQISGF
AYTYDSHQPLGHKIIEIRHQNKPLLREKMYTIATSDFLASGNGVFSVMKRGKIIHVGQSDHDTVIAYIKSLPQPFHASIE
ARIKESETGLKTGVG
>Mature_575_residues
MMFHRKSLYHYFNLFILLILGSSLNWASVNQHSTASPTLNIKILGINDFHGQISSGRAVKNEPVGGAAVLAAYLKEAQLG
MEDRSIITIMGDLVGASPPSSGLLNDEPSILFINGLGNNQCKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPP
TDSWISLPNYPGASYPYVSANIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADSMFPANAEGVKFLDEADSINHYI
PEIKAKGVNVIIVLIHEGGNQIPYEGDTQTTSAVEGHIKEIVYSLGDEIDVVMGGHTHQFLNAFLPNRNGHKILVTQANS
YSAAFAEVTLQIDTKNHTVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAEYDLLRKQNKDGESS
LGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNSIVTITLTGNDIYDLLEQQWMGSYINMLQISGF
AYTYDSHQPLGHKIIEIRHQNKPLLREKMYTIATSDFLASGNGVFSVMKRGKIIHVGQSDHDTVIAYIKSLPQPFHASIE
ARIKESETGLKTGVG

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=481, Percent_Identity=22.4532224532225, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790658, Length=612, Percent_Identity=22.2222222222222, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641187, Length=476, Percent_Identity=26.6806722689076, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI221329836, Length=476, Percent_Identity=24.7899159663866, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI28573524, Length=459, Percent_Identity=23.5294117647059, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922444, Length=493, Percent_Identity=21.501014198783, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922446, Length=477, Percent_Identity=22.6415094339623, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654424, Length=477, Percent_Identity=22.6415094339623, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 63018; Mature: 63018

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFHRKSLYHYFNLFILLILGSSLNWASVNQHSTASPTLNIKILGINDFHGQISSGRAVK
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCC
NEPVGGAAVLAAYLKEAQLGMEDRSIITIMGDLVGASPPSSGLLNDEPSILFINGLGNNQ
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
CKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPPTDSWISLPNYPGASYPYVSA
CCCHHHCCHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCCEEECCCCCCCCCCEEEE
NIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADSMFPANAEGVKFLDEADSINHYI
EEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
PEIKAKGVNVIIVLIHEGGNQIPYEGDTQTTSAVEGHIKEIVYSLGDEIDVVMGGHTHQF
CCHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
LNAFLPNRNGHKILVTQANSYSAAFAEVTLQIDTKNHTVKNKSAKIITTYANRWPGTNPD
HHHHCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCEECCCCCEEEEEECCCCCCCCCC
TQALEIVKLAEDKIGPIINSHVGTAEYDLLRKQNKDGESSLGNLIADAYRTVMNTDLGIT
HHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NPNGIRDDLKAGEVNWGNIYSILPFSNSIVTITLTGNDIYDLLEQQWMGSYINMLQISGF
CCCCCCHHHCCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHEECCE
AYTYDSHQPLGHKIIEIRHQNKPLLREKMYTIATSDFLASGNGVFSVMKRGKIIHVGQSD
EEEECCCCCCCCEEEEEECCCCCHHHHHHHHHEEHHHHCCCCCHHHHHHCCCEEEECCCC
HDTVIAYIKSLPQPFHASIEARIKESETGLKTGVG
CHHHHHHHHHCCCCCCCCHHHEEHHCCCCHHCCCC
>Mature Secondary Structure
MMFHRKSLYHYFNLFILLILGSSLNWASVNQHSTASPTLNIKILGINDFHGQISSGRAVK
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCC
NEPVGGAAVLAAYLKEAQLGMEDRSIITIMGDLVGASPPSSGLLNDEPSILFINGLGNNQ
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
CKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPPTDSWISLPNYPGASYPYVSA
CCCHHHCCHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCCEEECCCCCCCCCCEEEE
NIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADSMFPANAEGVKFLDEADSINHYI
EEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
PEIKAKGVNVIIVLIHEGGNQIPYEGDTQTTSAVEGHIKEIVYSLGDEIDVVMGGHTHQF
CCHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
LNAFLPNRNGHKILVTQANSYSAAFAEVTLQIDTKNHTVKNKSAKIITTYANRWPGTNPD
HHHHCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCEECCCCCEEEEEECCCCCCCCCC
TQALEIVKLAEDKIGPIINSHVGTAEYDLLRKQNKDGESSLGNLIADAYRTVMNTDLGIT
HHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NPNGIRDDLKAGEVNWGNIYSILPFSNSIVTITLTGNDIYDLLEQQWMGSYINMLQISGF
CCCCCCHHHCCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHEECCE
AYTYDSHQPLGHKIIEIRHQNKPLLREKMYTIATSDFLASGNGVFSVMKRGKIIHVGQSD
EEEECCCCCCCCEEEEEECCCCCHHHHHHHHHEEHHHHCCCCCHHHHHHCCCEEEECCCC
HDTVIAYIKSLPQPFHASIEARIKESETGLKTGVG
CHHHHHHHHHCCCCCCCCHHHEEHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]