| Definition | Vibrio cholerae O395 chromosome 2, complete sequence. |
|---|---|
| Accession | NC_009457 |
| Length | 3,024,069 |
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The map label for this gene is murF [H]
Identifier: 147673448
GI number: 147673448
Start: 2127886
End: 2129247
Strand: Reverse
Name: murF [H]
Synonym: VC0395_A1983
Alternate gene names: 147673448
Gene position: 2129247-2127886 (Counterclockwise)
Preceding gene: 147674384
Following gene: 147674771
Centisome position: 70.41
GC content: 50.73
Gene sequence:
>1362_bases ATGATTCGCGTAATGCTTTCTCAGCTCAGCTCAGTATTGAATGCTCGTTTAATTGGTGAGGATCGCGCGGTCAGTGCCGT ATCGACGGACAGTCGTGCTATTCCCGCCGAAGCGCTGTTTGTTGCCTTGGTCGGTGAACGTTTTGATGCGCACGATTTTG CCTCTCAAGCGGTAACTGGTGGCGCAGTGGCGCTGTTGGTTGAGCGTGAGCTCGATGCAGAGTGTTCACAACTGGTTGTT GCCGATACCAAACAGGCCTTAGGTCAGCTTGGCTCTTGGGTGCATGCGCAGTGTCAAACGCCTACGGTCGCGATCACCGG AAGTTGTGGCAAAACCACGGTCAAAGAGATGGTGGCCAGCATTTTAAGCCAGAAAGGCCAAGTGCTTTACACGCAAGGAA ACTTCAATAACGACATCGGCGTTCCGCTGACTCTGCTGCGCTCAACGACCGAAGATGATTATGCCGTGATTGAATTAGGG GCGAATCATGTAGGTGAAATTGCCTACACCACCGCCTTGGTTAAGCCGGATGTTGCCCTAGTCAACAACGTAGCGGCAGC GCACTTAGAAGGTTTTGGCTCGATGGATGGCGTTAAACGCGCTAAAGGTGAAATCTATCAAGGGTTACAGGCGGGTGCCG TGGCGATTGTTAATTTAGACAGCAACGGTGGTGACTACTGGAAAGCGGTACTGGCGGACAAAACCGTGAAAACATTTTCC GCTACCGATCACAATGCTGACTTTTATCCACGTGATGTGCGGTTAAATGCGCTCGGTATAGCTAGCTTTACTTTGGTTAC CCCGGTTGGTGAAGTGGCTGTTGAGTTGGGCATCATGGGCCAACACAACGTGGCCAATGCACTGGCTGCTGCGGCCTTAT CACTGGAAATGGGCGCGAGCTTGGCGGAAATTGGTCAAGGTTTAGCGCATTTATCCAAAGTGAAAGGTCGAGTGGATATG GCACCGTTGCGTGACGATATCACGCTTATTGATGACAGCTATAACGCTAGCGTGCCAGCCATGAAAGCGGCGGTGGAGCT GTTAGGCAGCTTTTCCGCTGTACGCTGGTTAATTTTGGGCAATATGGCGGAGTTGGGTGATGAAAGTCTTGCACTTCATC AGCAAGTCGGTGAACATGCTGCACCATTCGGTTTTGAGTTTGTCCTGACGTATGGCAAGGACGCAAAAGTGATCAGCGAC CTTTGCCAAGGGCATCACTTTGCTACCCATCAGGACATGATGACATTTATTGAGCAGCACATAGAACAACAACAGGGTCG TACTCAAGTATTGTTGGTGAAAGGTGCCAACAGTGCAGGGATGAGTAAAATTGCCGCTGCTCTCAAGGAGAAATTTGCAT GA
Upstream 100 bases:
>100_bases TGCTCGCGGGTAAAGGACATGAAGATTATCAGGTGCTGGCCAACGAAACGATTCACTACTCGGATCGTGAAACCGCCCAA CAGTTACTGGAGTTACAACC
Downstream 100 bases:
>100_bases TTATTTGGCTTGCTGAACTGCTCCAGCCATATTTTTCGTTTTTCCGTCTGTTCGAATATCTCTCATTTCGCGCCATTGTC AGCATTCTGACTGCGCTTGG
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 453; Mature: 453
Protein sequence:
>453_residues MIRVMLSQLSSVLNARLIGEDRAVSAVSTDSRAIPAEALFVALVGERFDAHDFASQAVTGGAVALLVERELDAECSQLVV ADTKQALGQLGSWVHAQCQTPTVAITGSCGKTTVKEMVASILSQKGQVLYTQGNFNNDIGVPLTLLRSTTEDDYAVIELG ANHVGEIAYTTALVKPDVALVNNVAAAHLEGFGSMDGVKRAKGEIYQGLQAGAVAIVNLDSNGGDYWKAVLADKTVKTFS ATDHNADFYPRDVRLNALGIASFTLVTPVGEVAVELGIMGQHNVANALAAAALSLEMGASLAEIGQGLAHLSKVKGRVDM APLRDDITLIDDSYNASVPAMKAAVELLGSFSAVRWLILGNMAELGDESLALHQQVGEHAAPFGFEFVLTYGKDAKVISD LCQGHHFATHQDMMTFIEQHIEQQQGRTQVLLVKGANSAGMSKIAAALKEKFA
Sequences:
>Translated_453_residues MIRVMLSQLSSVLNARLIGEDRAVSAVSTDSRAIPAEALFVALVGERFDAHDFASQAVTGGAVALLVERELDAECSQLVV ADTKQALGQLGSWVHAQCQTPTVAITGSCGKTTVKEMVASILSQKGQVLYTQGNFNNDIGVPLTLLRSTTEDDYAVIELG ANHVGEIAYTTALVKPDVALVNNVAAAHLEGFGSMDGVKRAKGEIYQGLQAGAVAIVNLDSNGGDYWKAVLADKTVKTFS ATDHNADFYPRDVRLNALGIASFTLVTPVGEVAVELGIMGQHNVANALAAAALSLEMGASLAEIGQGLAHLSKVKGRVDM APLRDDITLIDDSYNASVPAMKAAVELLGSFSAVRWLILGNMAELGDESLALHQQVGEHAAPFGFEFVLTYGKDAKVISD LCQGHHFATHQDMMTFIEQHIEQQQGRTQVLLVKGANSAGMSKIAAALKEKFA >Mature_453_residues MIRVMLSQLSSVLNARLIGEDRAVSAVSTDSRAIPAEALFVALVGERFDAHDFASQAVTGGAVALLVERELDAECSQLVV ADTKQALGQLGSWVHAQCQTPTVAITGSCGKTTVKEMVASILSQKGQVLYTQGNFNNDIGVPLTLLRSTTEDDYAVIELG ANHVGEIAYTTALVKPDVALVNNVAAAHLEGFGSMDGVKRAKGEIYQGLQAGAVAIVNLDSNGGDYWKAVLADKTVKTFS ATDHNADFYPRDVRLNALGIASFTLVTPVGEVAVELGIMGQHNVANALAAAALSLEMGASLAEIGQGLAHLSKVKGRVDM APLRDDITLIDDSYNASVPAMKAAVELLGSFSAVRWLILGNMAELGDESLALHQQVGEHAAPFGFEFVLTYGKDAKVISD LCQGHHFATHQDMMTFIEQHIEQQQGRTQVLLVKGANSAGMSKIAAALKEKFA
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=52.4336283185841, Blast_Score=396, Evalue=1e-111,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 47915; Mature: 47915
Theoretical pI: Translated: 5.02; Mature: 5.02
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIRVMLSQLSSVLNARLIGEDRAVSAVSTDSRAIPAEALFVALVGERFDAHDFASQAVTG CHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCC GAVALLVERELDAECSQLVVADTKQALGQLGSWVHAQCQTPTVAITGSCGKTTVKEMVAS CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHEEECCCCEEEEECCCCHHHHHHHHHH ILSQKGQVLYTQGNFNNDIGVPLTLLRSTTEDDYAVIELGANHVGEIAYTTALVKPDVAL HHCCCCCEEEEECCCCCCCCCCHHEECCCCCCCEEEEEECCCHHCHHHHHHHHCCCCHHH VNNVAAAHLEGFGSMDGVKRAKGEIYQGLQAGAVAIVNLDSNGGDYWKAVLADKTVKTFS HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCHHHEEE ATDHNADFYPRDVRLNALGIASFTLVTPVGEVAVELGIMGQHNVANALAAAALSLEMGAS CCCCCCCCCCCCEEEEEEEEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC LAEIGQGLAHLSKVKGRVDMAPLRDDITLIDDSYNASVPAMKAAVELLGSFSAVRWLILG HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC NMAELGDESLALHQQVGEHAAPFGFEFVLTYGKDAKVISDLCQGHHFATHQDMMTFIEQH CHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHH IEQQQGRTQVLLVKGANSAGMSKIAAALKEKFA HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCC >Mature Secondary Structure MIRVMLSQLSSVLNARLIGEDRAVSAVSTDSRAIPAEALFVALVGERFDAHDFASQAVTG CHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCC GAVALLVERELDAECSQLVVADTKQALGQLGSWVHAQCQTPTVAITGSCGKTTVKEMVAS CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHEEECCCCEEEEECCCCHHHHHHHHHH ILSQKGQVLYTQGNFNNDIGVPLTLLRSTTEDDYAVIELGANHVGEIAYTTALVKPDVAL HHCCCCCEEEEECCCCCCCCCCHHEECCCCCCCEEEEEECCCHHCHHHHHHHHCCCCHHH VNNVAAAHLEGFGSMDGVKRAKGEIYQGLQAGAVAIVNLDSNGGDYWKAVLADKTVKTFS HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCHHHEEE ATDHNADFYPRDVRLNALGIASFTLVTPVGEVAVELGIMGQHNVANALAAAALSLEMGAS CCCCCCCCCCCCEEEEEEEEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC LAEIGQGLAHLSKVKGRVDMAPLRDDITLIDDSYNASVPAMKAAVELLGSFSAVRWLILG HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC NMAELGDESLALHQQVGEHAAPFGFEFVLTYGKDAKVISDLCQGHHFATHQDMMTFIEQH CHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHH IEQQQGRTQVLLVKGANSAGMSKIAAALKEKFA HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]