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The map label for this gene is murE [H]

Identifier: 147674384

GI number: 147674384

Start: 2129244

End: 2130782

Strand: Reverse

Name: murE [H]

Synonym: VC0395_A1984

Alternate gene names: 147674384

Gene position: 2130782-2129244 (Counterclockwise)

Preceding gene: 147674985

Following gene: 147673448

Centisome position: 70.46

GC content: 52.76

Gene sequence:

>1539_bases
TTGCTCCTGATGAAAACCGCTTCCAAAACAAATAATAGTGTGAGACGGAACATGAACACGCATTCAGCGATCTCCGAACT
GATCGCGCCATGGCTAGAGCTGACCGACCCTAAGTTGGCCGCGCTGGTCATTACTCATCTTGAACTGGATAGCCGCCTGA
TTAAATCGGGTGATACCTTTGTGGCTATTCAAGGCCATGCAGTGGATGGTCGCCAATTTATCGATAAAGCCATTGCGCAA
GGTGCCAATCTTGTGTTGGCAGAAGCCGATGCTCAGCATCTCAATGGGTGGGTTGAGTATCGTGCTGGTGTCCCCGTCAT
CTATTTGGCTGAACTTGGTCAACATCTGTCAGAGTTGGCTGGGCGTCTGTATGGCGGCCACCACAATCAACTGATTGGCG
TGACGGGCACGAACGGTAAAACCACCATCACCCAGCTGATTGCTCAATGGCTCGAATTGCTCGGTCATAAAGCTGCCGTG
ATGGGCACCACGGGGAACGGTTTTTTAAATGCGCTTGAACCTGCGGCCAACACCACAGGTAACGCACTGCAAATTCAGGC
CACACTGCGCGATTTAGCCGAGCGCGGCGCGCAATATACGGCGCTGGAAACCTCGTCTCATGGTTTAGTGCAAGGGCGCG
TGAAAAAGCTCCATTTTGTGGCCGGTGTCTTTTCCAATTTGAGCCGTGACCATTTGGATTATCACGGCACTATGGAGGCT
TACGCCGCCGCGAAATTCTCTCTATTTAGTGAGCATGCCTGCCAAAACGCGATTATTAATGTCGATGATGCGGTCGGCGC
GCAATGGGTCAAACAATTGCCGCAGGCGATCGGGGTTTCCTTAGTGACGAAACCAAACACAGCGCAAGCCATCTGGGCGC
GCGAAGTGGCATACGCTGAAAGCGGTATTACGCTGAACTTTGAGAGTTCGTGGGGAGAAGGCGAATTGCACGCACCACTG
ATTGGTGAGTTCAATGCCTGTAACTTGTTATTGGCATTGGCAACCTTGCTCAGCTTGGGTTTTGAGAAAAGTGCACTGCT
CGCCACTGCGCCAAAGCTGCGTCCAGTCTTAGGACGGATGGAGCTATTCCAACGTGAGCAAAAAGCCAAAATGGTGGTCG
ATTATGCGCACACGCCGGACGCTTTAGAAAAAGCACTGCGCGCGTTACGTGTACATTGTGCGGGTCAGTTGTGGGCTATT
GTGGGGTGTGGCGGCGATCGTGATCGCGGTAAACGTCCGATGATGGCTGCGATTGCTGAGCAGTTTGCCGATCGCGTGAT
CTTGACCGATGACAACCCACGCAGTGAGTCGCCGCAAGCGATTGTAGCGGATATGGTTGCAGGACTGACTTACCCCGAGC
GCGCTCATATCGAACACCACCGTTTCCAAGCCGCGAGTTATGCGCTTCAGCATGCAGGTGCGCAAGACATCATCCTGCTC
GCGGGTAAAGGACATGAAGATTATCAGGTGCTGGCCAACGAAACGATTCACTACTCGGATCGTGAAACCGCCCAACAGTT
ACTGGAGTTACAACCATGA

Upstream 100 bases:

>100_bases
GTCATGGTCAATGAGCCGCAAGGCGACTCTTACTATGGCGGCGCAGTGGCCGGTCCGGTTTTCTCGGAAATCATGAAGGG
CGCATTACAAATCCTCAACA

Downstream 100 bases:

>100_bases
TTCGCGTAATGCTTTCTCAGCTCAGCTCAGTATTGAATGCTCGTTTAATTGGTGAGGATCGCGCGGTCAGTGCCGTATCG
ACGGACAGTCGTGCTATTCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLLMKTASKTNNSVRRNMNTHSAISELIAPWLELTDPKLAALVITHLELDSRLIKSGDTFVAIQGHAVDGRQFIDKAIAQ
GANLVLAEADAQHLNGWVEYRAGVPVIYLAELGQHLSELAGRLYGGHHNQLIGVTGTNGKTTITQLIAQWLELLGHKAAV
MGTTGNGFLNALEPAANTTGNALQIQATLRDLAERGAQYTALETSSHGLVQGRVKKLHFVAGVFSNLSRDHLDYHGTMEA
YAAAKFSLFSEHACQNAIINVDDAVGAQWVKQLPQAIGVSLVTKPNTAQAIWAREVAYAESGITLNFESSWGEGELHAPL
IGEFNACNLLLALATLLSLGFEKSALLATAPKLRPVLGRMELFQREQKAKMVVDYAHTPDALEKALRALRVHCAGQLWAI
VGCGGDRDRGKRPMMAAIAEQFADRVILTDDNPRSESPQAIVADMVAGLTYPERAHIEHHRFQAASYALQHAGAQDIILL
AGKGHEDYQVLANETIHYSDRETAQQLLELQP

Sequences:

>Translated_512_residues
MLLMKTASKTNNSVRRNMNTHSAISELIAPWLELTDPKLAALVITHLELDSRLIKSGDTFVAIQGHAVDGRQFIDKAIAQ
GANLVLAEADAQHLNGWVEYRAGVPVIYLAELGQHLSELAGRLYGGHHNQLIGVTGTNGKTTITQLIAQWLELLGHKAAV
MGTTGNGFLNALEPAANTTGNALQIQATLRDLAERGAQYTALETSSHGLVQGRVKKLHFVAGVFSNLSRDHLDYHGTMEA
YAAAKFSLFSEHACQNAIINVDDAVGAQWVKQLPQAIGVSLVTKPNTAQAIWAREVAYAESGITLNFESSWGEGELHAPL
IGEFNACNLLLALATLLSLGFEKSALLATAPKLRPVLGRMELFQREQKAKMVVDYAHTPDALEKALRALRVHCAGQLWAI
VGCGGDRDRGKRPMMAAIAEQFADRVILTDDNPRSESPQAIVADMVAGLTYPERAHIEHHRFQAASYALQHAGAQDIILL
AGKGHEDYQVLANETIHYSDRETAQQLLELQP
>Mature_512_residues
MLLMKTASKTNNSVRRNMNTHSAISELIAPWLELTDPKLAALVITHLELDSRLIKSGDTFVAIQGHAVDGRQFIDKAIAQ
GANLVLAEADAQHLNGWVEYRAGVPVIYLAELGQHLSELAGRLYGGHHNQLIGVTGTNGKTTITQLIAQWLELLGHKAAV
MGTTGNGFLNALEPAANTTGNALQIQATLRDLAERGAQYTALETSSHGLVQGRVKKLHFVAGVFSNLSRDHLDYHGTMEA
YAAAKFSLFSEHACQNAIINVDDAVGAQWVKQLPQAIGVSLVTKPNTAQAIWAREVAYAESGITLNFESSWGEGELHAPL
IGEFNACNLLLALATLLSLGFEKSALLATAPKLRPVLGRMELFQREQKAKMVVDYAHTPDALEKALRALRVHCAGQLWAI
VGCGGDRDRGKRPMMAAIAEQFADRVILTDDNPRSESPQAIVADMVAGLTYPERAHIEHHRFQAASYALQHAGAQDIILL
AGKGHEDYQVLANETIHYSDRETAQQLLELQP

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=490, Percent_Identity=53.0612244897959, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55616; Mature: 55616

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLMKTASKTNNSVRRNMNTHSAISELIAPWLELTDPKLAALVITHLELDSRLIKSGDTF
CEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEE
VAIQGHAVDGRQFIDKAIAQGANLVLAEADAQHLNGWVEYRAGVPVIYLAELGQHLSELA
EEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCHHHHCCCCCEEEHHHHHHHHHHHH
GRLYGGHHNQLIGVTGTNGKTTITQLIAQWLELLGHKAAVMGTTGNGFLNALEPAANTTG
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCC
NALQIQATLRDLAERGAQYTALETSSHGLVQGRVKKLHFVAGVFSNLSRDHLDYHGTMEA
CEEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHH
YAAAKFSLFSEHACQNAIINVDDAVGAQWVKQLPQAIGVSLVTKPNTAQAIWAREVAYAE
HHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHC
SGITLNFESSWGEGELHAPLIGEFNACNLLLALATLLSLGFEKSALLATAPKLRPVLGRM
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ELFQREQKAKMVVDYAHTPDALEKALRALRVHCAGQLWAIVGCGGDRDRGKRPMMAAIAE
HHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHH
QFADRVILTDDNPRSESPQAIVADMVAGLTYPERAHIEHHRFQAASYALQHAGAQDIILL
HHHCEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGKGHEDYQVLANETIHYSDRETAQQLLELQP
ECCCCCCHHHHHCCEEECCCHHHHHHHHCCCC
>Mature Secondary Structure
MLLMKTASKTNNSVRRNMNTHSAISELIAPWLELTDPKLAALVITHLELDSRLIKSGDTF
CEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEE
VAIQGHAVDGRQFIDKAIAQGANLVLAEADAQHLNGWVEYRAGVPVIYLAELGQHLSELA
EEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCHHHHCCCCCEEEHHHHHHHHHHHH
GRLYGGHHNQLIGVTGTNGKTTITQLIAQWLELLGHKAAVMGTTGNGFLNALEPAANTTG
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCC
NALQIQATLRDLAERGAQYTALETSSHGLVQGRVKKLHFVAGVFSNLSRDHLDYHGTMEA
CEEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHH
YAAAKFSLFSEHACQNAIINVDDAVGAQWVKQLPQAIGVSLVTKPNTAQAIWAREVAYAE
HHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHC
SGITLNFESSWGEGELHAPLIGEFNACNLLLALATLLSLGFEKSALLATAPKLRPVLGRM
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ELFQREQKAKMVVDYAHTPDALEKALRALRVHCAGQLWAIVGCGGDRDRGKRPMMAAIAE
HHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHH
QFADRVILTDDNPRSESPQAIVADMVAGLTYPERAHIEHHRFQAASYALQHAGAQDIILL
HHHCEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGKGHEDYQVLANETIHYSDRETAQQLLELQP
ECCCCCCHHHHHCCEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]