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The map label for this gene is fadE25 [H]

Identifier: 146343861

GI number: 146343861

Start: 7423897

End: 7425093

Strand: Reverse

Name: fadE25 [H]

Synonym: BRADO7111

Alternate gene names: 146343861

Gene position: 7425093-7423897 (Counterclockwise)

Preceding gene: 146343862

Following gene: 146343860

Centisome position: 99.58

GC content: 70.26

Gene sequence:

>1197_bases
ATGAGTGCATCGGCGAAGGTGATTCCCCGCGACTGGCTGGACTGGCCGTTTTTCGAACCGCGGCACCGCGACCTGTGCAA
GCGGCTTGATCGTTTCGTCGGCTCGGGCGTGCTCGACGACGTCGATCACGGCGATGTCGACGCGGCGTGTCGCGGACTCG
TGCGCGCGCTCGGCGCCGCGGGATTGCTCGACAGCGCTGTCGTGGCGCCGGATGCGGATGCTGGCGCGATCGATTCCCGT
GCGATCTGCCTGTCGCGCGAAACGCTGGCTTACGCGGACGGCCTCGCCGATTTCGCCTTCGCCATGCAGGGGCTCGGCTC
CGGCGCGATCGCGCTCGGCGGCTCCGAGGAGCTGCGCGCAGGCGTGCTGCCGAAGGTGCGATCGGGCGAATGGCTGGCGG
CCTTCGCGCTGTCGGAGAAGGAGGCCGGCTCCGACGTCGCCGCGATGGCCTGCAGCGCCCGGCGCGACGGCGACGACTAC
GTGCTCGACGGCGAGAAGACCTGGATCTCCAATGGCGGTATCGCCGACGTCTATACGCTGTTCGCCCGCACCGGCGAGGC
ACCGGGCGCGCGCGGCATCTCCGCCTTCGTCGTGTATCCCGATGATTCCGGCTTCTCGGTCGCCGAGCGCATCGAGGTGA
TCGCGCCGCATCCGCTGGCGACCTTGCGCCTCGAGAACTGCCGCATTCCGGTGAGCCGGCGGCTCGGCGCGCCCGGTGGC
GGCTTCAAGCTCGCCATGCAGACGCTCGACATCTTCCGCGCCTCTGTCGCCGCCGCCGCGCTCGGCTTCGGCCGCCGCGC
CCTCGACGAGGCGCAGGCGCATGCGCAGAGCCGCCAAATGTTCGGCGGCGCCTTGGCCGACCTGCAGATGACGCAGGCCG
CCTTTGGCGAGATGGCCACCGACACCGACGCCGCGGCGCTCCTGACCTACCGGGCCGCCTGGCGCCGCGACGTCCAGAAG
CTGCCGACCACGCGTGAGGCCGCCATGGCCAAGCTCACTGCCACCGAGACCGCGCAACGCGTCATCGACCGTGCGCTGCA
GATGTTCGGCGGCCGCGGCGTCCGCAAGGGTGAGATCGTGGAAGGCCTTTACCGCGAGATCCGCGCGCTGCGCATCTACG
AGGGCGCGACCGAAGTGCAGAAGCTGATCGTCGCGCGCGAATTCCTGAAGTCGCGTGCCGGCGTGGTACGCGACTGA

Upstream 100 bases:

>100_bases
GCGACGGTCTTTGCACCTGCCCGGAGCCCGACGAAAGCTTGACAGGGCGATCTGCCGCCAATTATTTTATGTTTAAAATA
ATTTGCGGACGGGTCCTGTC

Downstream 100 bases:

>100_bases
TCATTGCGCCACGCGGCACCCGTCGAGCTTCCGCGGCCGGCGGCATCCCCACCGGCGCGTTCATGCGCAGATCAGTCCAG
CGGCGGTTCGCGAGCCGCAG

Product: putative acyl-CoA dehydrogenase

Products: 2-butenoyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSASAKVIPRDWLDWPFFEPRHRDLCKRLDRFVGSGVLDDVDHGDVDAACRGLVRALGAAGLLDSAVVAPDADAGAIDSR
AICLSRETLAYADGLADFAFAMQGLGSGAIALGGSEELRAGVLPKVRSGEWLAAFALSEKEAGSDVAAMACSARRDGDDY
VLDGEKTWISNGGIADVYTLFARTGEAPGARGISAFVVYPDDSGFSVAERIEVIAPHPLATLRLENCRIPVSRRLGAPGG
GFKLAMQTLDIFRASVAAAALGFGRRALDEAQAHAQSRQMFGGALADLQMTQAAFGEMATDTDAAALLTYRAAWRRDVQK
LPTTREAAMAKLTATETAQRVIDRALQMFGGRGVRKGEIVEGLYREIRALRIYEGATEVQKLIVAREFLKSRAGVVRD

Sequences:

>Translated_398_residues
MSASAKVIPRDWLDWPFFEPRHRDLCKRLDRFVGSGVLDDVDHGDVDAACRGLVRALGAAGLLDSAVVAPDADAGAIDSR
AICLSRETLAYADGLADFAFAMQGLGSGAIALGGSEELRAGVLPKVRSGEWLAAFALSEKEAGSDVAAMACSARRDGDDY
VLDGEKTWISNGGIADVYTLFARTGEAPGARGISAFVVYPDDSGFSVAERIEVIAPHPLATLRLENCRIPVSRRLGAPGG
GFKLAMQTLDIFRASVAAAALGFGRRALDEAQAHAQSRQMFGGALADLQMTQAAFGEMATDTDAAALLTYRAAWRRDVQK
LPTTREAAMAKLTATETAQRVIDRALQMFGGRGVRKGEIVEGLYREIRALRIYEGATEVQKLIVAREFLKSRAGVVRD
>Mature_397_residues
SASAKVIPRDWLDWPFFEPRHRDLCKRLDRFVGSGVLDDVDHGDVDAACRGLVRALGAAGLLDSAVVAPDADAGAIDSRA
ICLSRETLAYADGLADFAFAMQGLGSGAIALGGSEELRAGVLPKVRSGEWLAAFALSEKEAGSDVAAMACSARRDGDDYV
LDGEKTWISNGGIADVYTLFARTGEAPGARGISAFVVYPDDSGFSVAERIEVIAPHPLATLRLENCRIPVSRRLGAPGGG
FKLAMQTLDIFRASVAAAALGFGRRALDEAQAHAQSRQMFGGALADLQMTQAAFGEMATDTDAAALLTYRAAWRRDVQKL
PTTREAAMAKLTATETAQRVIDRALQMFGGRGVRKGEIVEGLYREIRALRIYEGATEVQKLIVAREFLKSRAGVVRD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=344, Percent_Identity=38.953488372093, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI4557231, Length=344, Percent_Identity=33.1395348837209, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI187960098, Length=344, Percent_Identity=33.1395348837209, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI7656849, Length=279, Percent_Identity=33.6917562724014, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI21361497, Length=295, Percent_Identity=34.5762711864407, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI226958412, Length=356, Percent_Identity=30.0561797752809, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI226958414, Length=357, Percent_Identity=30.2521008403361, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI4501859, Length=340, Percent_Identity=32.0588235294118, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI4557235, Length=294, Percent_Identity=34.6938775510204, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI76496475, Length=294, Percent_Identity=34.6938775510204, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4501857, Length=380, Percent_Identity=27.8947368421053, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI48976061, Length=290, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI211971008, Length=290, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503943, Length=292, Percent_Identity=27.7397260273973, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI7669494, Length=280, Percent_Identity=27.5, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081958, Length=286, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786223, Length=287, Percent_Identity=27.1777003484321, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082384, Length=297, Percent_Identity=27.9461279461279, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17533517, Length=279, Percent_Identity=35.1254480286738, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17570075, Length=345, Percent_Identity=32.1739130434783, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17569725, Length=343, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17534899, Length=347, Percent_Identity=30.835734870317, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17508101, Length=275, Percent_Identity=36, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538396, Length=356, Percent_Identity=31.4606741573034, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71985184, Length=358, Percent_Identity=31.8435754189944, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17506239, Length=275, Percent_Identity=33.8181818181818, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71990804, Length=224, Percent_Identity=35.7142857142857, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71985192, Length=305, Percent_Identity=30.4918032786885, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17551932, Length=285, Percent_Identity=30.5263157894737, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17505929, Length=289, Percent_Identity=30.4498269896194, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32563615, Length=179, Percent_Identity=32.9608938547486, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71982178, Length=290, Percent_Identity=27.9310344827586, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17531909, Length=240, Percent_Identity=27.9166666666667, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562446, Length=357, Percent_Identity=27.1708683473389, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534353, Length=340, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI86563383, Length=322, Percent_Identity=27.0186335403727, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI86563381, Length=322, Percent_Identity=27.0186335403727, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24646207, Length=372, Percent_Identity=35.752688172043, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI21356377, Length=344, Percent_Identity=37.2093023255814, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24660351, Length=339, Percent_Identity=32.7433628318584, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281363737, Length=294, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24666513, Length=282, Percent_Identity=35.1063829787234, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21355753, Length=354, Percent_Identity=29.0960451977401, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19920834, Length=289, Percent_Identity=28.0276816608997, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.2

Molecular weight: Translated: 42510; Mature: 42379

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAKVIPRDWLDWPFFEPRHRDLCKRLDRFVGSGVLDDVDHGDVDAACRGLVRALGAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
GLLDSAVVAPDADAGAIDSRAICLSRETLAYADGLADFAFAMQGLGSGAIALGGSEELRA
HHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
GVLPKVRSGEWLAAFALSEKEAGSDVAAMACSARRDGDDYVLDGEKTWISNGGIADVYTL
CCCCCCCCCCEEEEEEECCHHCCCHHHHHHHHHCCCCCCEEECCCEEEECCCCHHHHHHH
FARTGEAPGARGISAFVVYPDDSGFSVAERIEVIAPHPLATLRLENCRIPVSRRLGAPGG
HHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCHHEEEECCCCCCHHHHCCCCCC
GFKLAMQTLDIFRASVAAAALGFGRRALDEAQAHAQSRQMFGGALADLQMTQAAFGEMAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTDAAALLTYRAAWRRDVQKLPTTREAAMAKLTATETAQRVIDRALQMFGGRGVRKGEIV
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EGLYREIRALRIYEGATEVQKLIVAREFLKSRAGVVRD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SASAKVIPRDWLDWPFFEPRHRDLCKRLDRFVGSGVLDDVDHGDVDAACRGLVRALGAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
GLLDSAVVAPDADAGAIDSRAICLSRETLAYADGLADFAFAMQGLGSGAIALGGSEELRA
HHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
GVLPKVRSGEWLAAFALSEKEAGSDVAAMACSARRDGDDYVLDGEKTWISNGGIADVYTL
CCCCCCCCCCEEEEEEECCHHCCCHHHHHHHHHCCCCCCEEECCCEEEECCCCHHHHHHH
FARTGEAPGARGISAFVVYPDDSGFSVAERIEVIAPHPLATLRLENCRIPVSRRLGAPGG
HHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCHHEEEECCCCCCHHHHCCCCCC
GFKLAMQTLDIFRASVAAAALGFGRRALDEAQAHAQSRQMFGGALADLQMTQAAFGEMAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTDAAALLTYRAAWRRDVQKLPTTREAAMAKLTATETAQRVIDRALQMFGGRGVRKGEIV
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EGLYREIRALRIYEGATEVQKLIVAREFLKSRAGVVRD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: butanoyl-CoA; acceptor

Specific reaction: butanoyl-CoA + acceptor = 2-butenoyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]