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The map label for this gene is amiF [H]

Identifier: 146343862

GI number: 146343862

Start: 7425345

End: 7426358

Strand: Reverse

Name: amiF [H]

Synonym: BRADO7112

Alternate gene names: 146343862

Gene position: 7426358-7425345 (Counterclockwise)

Preceding gene: 146343863

Following gene: 146343861

Centisome position: 99.59

GC content: 62.92

Gene sequence:

>1014_bases
ATGAATGGGCTCGGCGGTCTCAACAAATCCGAACACGGCGTGGTCATCGGCCTCGTGCAACTCCAATTGCCGGTCGTCGT
GACCAAGGAGGATCTCGCGAAGCAGACCGAGAAGATCGTCTGGATGGTCGGCAAGGCCCGGCGCAATCTCGGCACCATGG
ACCTCGTCGTCTTCCCGGAATATTCGCTGCACGGGCTGTCGATGGACACCAATCCGGAGATCATGTGCCGGCTGGATGGG
CCGGAGGTCGCGGCGTTCAAGCAGGCCTGCATCGACAACAAGATCTGGGGTTGCTTCTCCATCATGGAGTACAACCCCGA
CGGCAATCCCTACAATTCCGGCCTGATCATCGACAGCAATGGCGAGATCAAGCTGTACTATCGCAAGCTTCATCCGTGGA
TCCCGGTCGAGCCGTGGGAGCCCGGCGATCTCGGCATTCCCGTCATCGAGGGGCCGCGCGGCGCCAAGATCGCGCTGATC
ATCTGCCATGACGGCATGTTCCCGGAAATGGCGCGGGAATGCGCCTACAAGGGCGCGGAGATCATGATCCGCACGGCGGG
CTACACCGCCCCGATCCGCGACAGCTGGCGCTTCACCAACCAGGCGAATGCATTCCAGAATCTCATGGTGACCGCCAACG
TCTGTATGTGCGGCTCCGACGGCTCGTTCGATTCGATGGGCGAGGGCATGATCGTCAATTTCGACGGCAGCATTCTCGCG
CACGGCACGACCGGCCGGGCCGACGAGATTATCACCGCGGAGGTGCGTCCCGATCTCGTGCGCGAGGCGCGCATCGGCTG
GGGCGTCGAGAACAACATCTATCAGCTCTGGCACCGCGGCTATGTCGCGGTGAAGGGCGGCGCGATGGATTGCCCGTACA
CCTTCATGCACGACATGGTCGCCGGCACCTACCGGCTGCCCTGGGAGGACCAGGTCAAGATCACCGACGGCACCTCCTGC
GGCTTCCCCGCGCCGACGCGCGTGTTCGGAAAGATGGCCAAGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CGCAATCGGCAGCGCGCGCTGAAATATCGGCGCGCGGCGTCCGCCAAATCGTGGTTGTCTTGGTCAAAATTCAGGTGGCA
GCCACACAGGGAGGTCCGGC

Downstream 100 bases:

>100_bases
AGGGACGGCGGGCCCTCCCTGAGCCTGTCGCATGCGGCGATCAGAGGTTTGCGCCCGTCACTCTCCACATCGTCATGACC
GGAACAAGCCCGGGCATGAC

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDG
PEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALI
ICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILA
HGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSC
GFPAPTRVFGKMAKAAE

Sequences:

>Translated_337_residues
MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDG
PEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALI
ICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILA
HGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSC
GFPAPTRVFGKMAKAAE
>Mature_337_residues
MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDG
PEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALI
ICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILA
HGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSC
GFPAPTRVFGKMAKAAE

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 37205; Mature: 37205

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPE
CCCCCCCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHHHEEEEEEHHHCCCCCEEEEEECC
YSLHGLSMDTNPEIMCRLDGPEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSN
CCEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEECC
GEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALIICHDGMFPEMARECAYKGAE
CCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHCCCCE
IMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILA
EEEEECCCCCCCCCCCCCCCHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEEECCCEEE
HGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMV
ECCCCCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHH
AGTYRLPWEDQVKITDGTSCGFPAPTRVFGKMAKAAE
HCEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPE
CCCCCCCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHHHEEEEEEHHHCCCCCEEEEEECC
YSLHGLSMDTNPEIMCRLDGPEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSN
CCEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEECC
GEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALIICHDGMFPEMARECAYKGAE
CCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHCCCCE
IMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILA
EEEEECCCCCCCCCCCCCCCHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEEECCCEEE
HGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMV
ECCCCCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHH
AGTYRLPWEDQVKITDGTSCGFPAPTRVFGKMAKAAE
HCEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]