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The map label for this gene is metY [H]

Identifier: 146343669

GI number: 146343669

Start: 7182105

End: 7183403

Strand: Direct

Name: metY [H]

Synonym: BRADO6912

Alternate gene names: 146343669

Gene position: 7182105-7183403 (Clockwise)

Preceding gene: 146343668

Following gene: 146343670

Centisome position: 96.32

GC content: 65.59

Gene sequence:

>1299_bases
ATGCGCAACGAGACGATTGCAATCCACGCCGGCTACGAGCCCGAGGGCACGACCAAGGCCGTTGCGGTCCCGATCTATCA
GACCGCCGCCTATGCGTTCGACAGTGCCGACCACGGCGCCGCCCTGTTCAATCTCGAAGTCGAAGGCTACCGTTACAGCC
GCATCTCCAATCCCACGACGGCCGTGCTCGAGAAGCGTGTCGCCGAGCTTGAAGGCGGTGTCGGCGCGCTCGCCGTCGGC
ACCGGCCAGGCCGCGCTGCATTTCGCGTTCGTCAATCTCGCCGATGGCGGCGGCAACATCGTCTCGGTGCCGCAGCTTTA
TGGCACGACCCACACGTTGCTCGGCCACATTCTGCCGCGCCAGGGCATCACCGGCCGCTTTGCCGAGAGCGACCAGCCCG
ACGCGATCGAACGCCTGATCGACGCGAACACCAAGGCCGTGTTCGCCGAGACGATCGGCAATCCGGCCGGCAATGTGTGC
GACATCGAGGCGCTGGCAAAGGTCGCGCATCGCAATGGCGTGCCGCTGATCGTGGACAACACCGTCGCAACGCCGATCCT
GCTGCGGCCGTTCGACTACGGCGCCGACATCGCCGTGCACTCGCTGACCAAATTTCTCGGCGGCCACGGCACCACGCTCG
GCGGCATGCTGGTCGACAGCGGCAACTTCCCTTGGAAGGATCACGCCGATCGCTTCCCGGCCTTCAGCCAGCCCGACACC
TCCTATCACGGCATGGTCTACACCGACCATTTCGGCCGCAGCGCCTATATCCAGCGGGCCCGCAGCGTTTATCAGCGCAC
GATGGGCGCGGTGCTGTCGCCGTTCAACGCCTTCCTGCTGCTGCAGGGCATCGAGACGGTGGCGCTGCGCATGGAGCGTC
ACGTCGAGAACGCGCGCAAGGTCGCCGAATTTCTCCACAGGGATTCGCGCGTCGCGTGGGTCAACTACGCGGGCTTTCCC
GACAGTCCGTATTATCCGCTGGTGCAGAAATATCTCGACGGCCGCGCCTCCTCGCTGTTCACGTTCGGGATCAAGGGCGG
CCTGGAAGCCGGCAAGGCGTTCTACGACGCACTCAAGCTGATCACGCGGCTGGTCAATATCGGCGATGCCAAGTCGCTCG
CCTGCCATCCGGCGTCGACGACGCACCGGCAGATGCCGCCGGAGGACCAGCGCAAGGCCGGCGTGCCGCCGGAAGCCATC
CGGCTGTCGATCGGCATCGAGCATATCGACGACATCATCGAAGATCTCGACCAGGCGCTGGCCCTGGCCTGCCCGCGTCA
GCTGGACGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
CGCGAATTATGGACCGCTTTACGCCTACACTCCTACAACATCGGTACTCGCGGATGACGTGCAGACCGCTTCGTCGATCG
AAGCGGCACGGAGATTGCCG

Downstream 100 bases:

>100_bases
AGCGATCAGCCGATGACCCTGCTGTTCGACGGAGATCGCAGGATCAAGAGCCCTGCTCTCGCGCCCGCCGGCGGCGACCG
TCGCGCGCGCGACCCGATCG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRNETIAIHAGYEPEGTTKAVAVPIYQTAAYAFDSADHGAALFNLEVEGYRYSRISNPTTAVLEKRVAELEGGVGALAVG
TGQAALHFAFVNLADGGGNIVSVPQLYGTTHTLLGHILPRQGITGRFAESDQPDAIERLIDANTKAVFAETIGNPAGNVC
DIEALAKVAHRNGVPLIVDNTVATPILLRPFDYGADIAVHSLTKFLGGHGTTLGGMLVDSGNFPWKDHADRFPAFSQPDT
SYHGMVYTDHFGRSAYIQRARSVYQRTMGAVLSPFNAFLLLQGIETVALRMERHVENARKVAEFLHRDSRVAWVNYAGFP
DSPYYPLVQKYLDGRASSLFTFGIKGGLEAGKAFYDALKLITRLVNIGDAKSLACHPASTTHRQMPPEDQRKAGVPPEAI
RLSIGIEHIDDIIEDLDQALALACPRQLDAAE

Sequences:

>Translated_432_residues
MRNETIAIHAGYEPEGTTKAVAVPIYQTAAYAFDSADHGAALFNLEVEGYRYSRISNPTTAVLEKRVAELEGGVGALAVG
TGQAALHFAFVNLADGGGNIVSVPQLYGTTHTLLGHILPRQGITGRFAESDQPDAIERLIDANTKAVFAETIGNPAGNVC
DIEALAKVAHRNGVPLIVDNTVATPILLRPFDYGADIAVHSLTKFLGGHGTTLGGMLVDSGNFPWKDHADRFPAFSQPDT
SYHGMVYTDHFGRSAYIQRARSVYQRTMGAVLSPFNAFLLLQGIETVALRMERHVENARKVAEFLHRDSRVAWVNYAGFP
DSPYYPLVQKYLDGRASSLFTFGIKGGLEAGKAFYDALKLITRLVNIGDAKSLACHPASTTHRQMPPEDQRKAGVPPEAI
RLSIGIEHIDDIIEDLDQALALACPRQLDAAE
>Mature_432_residues
MRNETIAIHAGYEPEGTTKAVAVPIYQTAAYAFDSADHGAALFNLEVEGYRYSRISNPTTAVLEKRVAELEGGVGALAVG
TGQAALHFAFVNLADGGGNIVSVPQLYGTTHTLLGHILPRQGITGRFAESDQPDAIERLIDANTKAVFAETIGNPAGNVC
DIEALAKVAHRNGVPLIVDNTVATPILLRPFDYGADIAVHSLTKFLGGHGTTLGGMLVDSGNFPWKDHADRFPAFSQPDT
SYHGMVYTDHFGRSAYIQRARSVYQRTMGAVLSPFNAFLLLQGIETVALRMERHVENARKVAEFLHRDSRVAWVNYAGFP
DSPYYPLVQKYLDGRASSLFTFGIKGGLEAGKAFYDALKLITRLVNIGDAKSLACHPASTTHRQMPPEDQRKAGVPPEAI
RLSIGIEHIDDIIEDLDQALALACPRQLDAAE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=33.806146572104, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=31.9148936170213, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=29.6208530805687, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790375, Length=417, Percent_Identity=28.0575539568345, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=25.9433962264151, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=30.9178743961353, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=32.7868852459016, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=425, Percent_Identity=33.8823529411765, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=430, Percent_Identity=46.5116279069767, Blast_Score=353, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6319307, Length=381, Percent_Identity=33.5958005249344, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=23.1981981981982, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=339, Percent_Identity=26.5486725663717, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=26.5734265734266, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=32.387706855792, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46766; Mature: 46766

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNETIAIHAGYEPEGTTKAVAVPIYQTAAYAFDSADHGAALFNLEVEGYRYSRISNPTT
CCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEEECCEEEECCCCCHH
AVLEKRVAELEGGVGALAVGTGQAALHFAFVNLADGGGNIVSVPQLYGTTHTLLGHILPR
HHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCCCEEECHHHHCCHHHHHHHHHCC
QGITGRFAESDQPDAIERLIDANTKAVFAETIGNPAGNVCDIEALAKVAHRNGVPLIVDN
CCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHCCCCCCEECHHHHHHHHHCCCCCEEECC
TVATPILLRPFDYGADIAVHSLTKFLGGHGTTLGGMLVDSGNFPWKDHADRFPAFSQPDT
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCCCCCCCCCCC
SYHGMVYTDHFGRSAYIQRARSVYQRTMGAVLSPFNAFLLLQGIETVALRMERHVENARK
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEFLHRDSRVAWVNYAGFPDSPYYPLVQKYLDGRASSLFTFGIKGGLEAGKAFYDALKL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHHHHH
ITRLVNIGDAKSLACHPASTTHRQMPPEDQRKAGVPPEAIRLSIGIEHIDDIIEDLDQAL
HHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCHHHEEEEECHHHHHHHHHHHHHHH
ALACPRQLDAAE
HHHCCCCCCCCC
>Mature Secondary Structure
MRNETIAIHAGYEPEGTTKAVAVPIYQTAAYAFDSADHGAALFNLEVEGYRYSRISNPTT
CCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEEECCEEEECCCCCHH
AVLEKRVAELEGGVGALAVGTGQAALHFAFVNLADGGGNIVSVPQLYGTTHTLLGHILPR
HHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCCCEEECHHHHCCHHHHHHHHHCC
QGITGRFAESDQPDAIERLIDANTKAVFAETIGNPAGNVCDIEALAKVAHRNGVPLIVDN
CCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHCCCCCCEECHHHHHHHHHCCCCCEEECC
TVATPILLRPFDYGADIAVHSLTKFLGGHGTTLGGMLVDSGNFPWKDHADRFPAFSQPDT
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCCCCCCCCCCC
SYHGMVYTDHFGRSAYIQRARSVYQRTMGAVLSPFNAFLLLQGIETVALRMERHVENARK
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEFLHRDSRVAWVNYAGFPDSPYYPLVQKYLDGRASSLFTFGIKGGLEAGKAFYDALKL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHHHHH
ITRLVNIGDAKSLACHPASTTHRQMPPEDQRKAGVPPEAIRLSIGIEHIDDIIEDLDQAL
HHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCHHHEEEEECHHHHHHHHHHHHHHH
ALACPRQLDAAE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]