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The map label for this gene is ispH [H]

Identifier: 146343361

GI number: 146343361

Start: 6838230

End: 6839171

Strand: Reverse

Name: ispH [H]

Synonym: BRADO6588

Alternate gene names: 146343361

Gene position: 6839171-6838230 (Counterclockwise)

Preceding gene: 146343363

Following gene: 146343360

Centisome position: 91.72

GC content: 67.3

Gene sequence:

>942_bases
GTGCTCTGCTCGCCGCGCGGCTTCTGTGCCGGCGTGGTCCGCGCCATCGATACCGTGGAACGGGCGCTCGCCATCTATGG
CGCCCCGGTCTATGTCCGGCACGAGATCGTGCACAATAAATACGTCGTCGACAGCCTGAAGACCAAGGGCGCGATCTTCG
TCGAGGAACTGGCCGAGATCCCCGACAATACCAATGCACCGGTGGTGTTCTCGGCCCACGGCGTGCCGAAATCGGTTCCC
GCCGAGGCCCAGGCGCGCAATTTCTTCTCGCTCGACGCGACCTGCCCGCTGGTCACCAAGGTGCACCGCGAGGCCGCCAT
TCACTTCAAGCGCGGCCGCGAGATCCTGCTGATCGGCCATTCCCATCACCCGGAGGTAGTCGGCACCGTCGGCCAGCTGC
CGCCGGGCGCCGTGACCCTGATCGAGACCGCCGAGGATGCCGCGACCTTCACCCCGAAGGATCCGAACAACCTCGCTTTC
GTCACCCAGACCACGCTGTCGATCGACGATACCGCCGACATCGTGGCCGTGCTCAAGGGCCGCTTCCCGAACATCTCCGG
CCCGCACAAGGAAGACATCTGCTACGCCACCACCAACCGCCAGCTGGCGGTGAAGAAGGTGGCCCCGGTGGTCGACGCCC
TGATCGTGGTCGGCGCGCCGAACTCGTCGAACTCGCAGCGCCTGCGCGAGGTCGCCGAGCGCGAGGGCTGCCCGATCGCG
GTGCTGGCGCAGCGCGCCTCGGATATCGACTGGGCGCGCTTCGAGGGCATCAAGAGCCTCGGCATCACCGCCGGCGCCTC
GGCCCCCGAGGTGATCGTCGAGGAGATCATGGGCGCGTTTGCCGAGCGCTACGTGCTGCATGTCGAGACCGTCTCCGCCG
CGGAAGAAAACGAATTCTTCCCGCTGCCGCGCTCGCTGCGCCCGGAAGCGGCCGCCGAGTAG

Upstream 100 bases:

>100_bases
GACCGCCCCCGCTTGCCGGCCCGGGGTGCCCCGGTCGCCGCAACAAGCCCGCACAGATCAGCTCAGACCGCCCATGGCAG
CTAAACCAGACCTCAAAATC

Downstream 100 bases:

>100_bases
GCCGGCGGTTCTTTTCCACCCGCCGGCGGGCCATCGGCCTTGAACGCGACGCCCGCATGCGCGATGAAGGCGCAACGGGC
CTGTCCGGTGACGAAGTTCG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLCSPRGFCAGVVRAIDTVERALAIYGAPVYVRHEIVHNKYVVDSLKTKGAIFVEELAEIPDNTNAPVVFSAHGVPKSVP
AEAQARNFFSLDATCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTVGQLPPGAVTLIETAEDAATFTPKDPNNLAF
VTQTTLSIDDTADIVAVLKGRFPNISGPHKEDICYATTNRQLAVKKVAPVVDALIVVGAPNSSNSQRLREVAEREGCPIA
VLAQRASDIDWARFEGIKSLGITAGASAPEVIVEEIMGAFAERYVLHVETVSAAEENEFFPLPRSLRPEAAAE

Sequences:

>Translated_313_residues
MLCSPRGFCAGVVRAIDTVERALAIYGAPVYVRHEIVHNKYVVDSLKTKGAIFVEELAEIPDNTNAPVVFSAHGVPKSVP
AEAQARNFFSLDATCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTVGQLPPGAVTLIETAEDAATFTPKDPNNLAF
VTQTTLSIDDTADIVAVLKGRFPNISGPHKEDICYATTNRQLAVKKVAPVVDALIVVGAPNSSNSQRLREVAEREGCPIA
VLAQRASDIDWARFEGIKSLGITAGASAPEVIVEEIMGAFAERYVLHVETVSAAEENEFFPLPRSLRPEAAAE
>Mature_313_residues
MLCSPRGFCAGVVRAIDTVERALAIYGAPVYVRHEIVHNKYVVDSLKTKGAIFVEELAEIPDNTNAPVVFSAHGVPKSVP
AEAQARNFFSLDATCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTVGQLPPGAVTLIETAEDAATFTPKDPNNLAF
VTQTTLSIDDTADIVAVLKGRFPNISGPHKEDICYATTNRQLAVKKVAPVVDALIVVGAPNSSNSQRLREVAEREGCPIA
VLAQRASDIDWARFEGIKSLGITAGASAPEVIVEEIMGAFAERYVLHVETVSAAEENEFFPLPRSLRPEAAAE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=51.4563106796116, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 33684; Mature: 33684

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCSPRGFCAGVVRAIDTVERALAIYGAPVYVRHEIVHNKYVVDSLKTKGAIFVEELAEI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHHHHHHCCCCCHHHHHHHHC
PDNTNAPVVFSAHGVPKSVPAEAQARNFFSLDATCPLVTKVHREAAIHFKRGREILLIGH
CCCCCCCEEEEECCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEC
SHHPEVVGTVGQLPPGAVTLIETAEDAATFTPKDPNNLAFVTQTTLSIDDTADIVAVLKG
CCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHC
RFPNISGPHKEDICYATTNRQLAVKKVAPVVDALIVVGAPNSSNSQRLREVAEREGCPIA
CCCCCCCCCCCCEEEEECCCEEHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCEE
VLAQRASDIDWARFEGIKSLGITAGASAPEVIVEEIMGAFAERYVLHVETVSAAEENEFF
EEEHHCCCCCHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC
PLPRSLRPEAAAE
CCCCCCCCCCCCC
>Mature Secondary Structure
MLCSPRGFCAGVVRAIDTVERALAIYGAPVYVRHEIVHNKYVVDSLKTKGAIFVEELAEI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHHHHHHCCCCCHHHHHHHHC
PDNTNAPVVFSAHGVPKSVPAEAQARNFFSLDATCPLVTKVHREAAIHFKRGREILLIGH
CCCCCCCEEEEECCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEC
SHHPEVVGTVGQLPPGAVTLIETAEDAATFTPKDPNNLAFVTQTTLSIDDTADIVAVLKG
CCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHC
RFPNISGPHKEDICYATTNRQLAVKKVAPVVDALIVVGAPNSSNSQRLREVAEREGCPIA
CCCCCCCCCCCCEEEEECCCEEHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCEE
VLAQRASDIDWARFEGIKSLGITAGASAPEVIVEEIMGAFAERYVLHVETVSAAEENEFF
EEEHHCCCCCHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC
PLPRSLRPEAAAE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]