Definition Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession NC_009445
Length 7,456,587

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The map label for this gene is 146343216

Identifier: 146343216

GI number: 146343216

Start: 6679304

End: 6680155

Strand: Direct

Name: 146343216

Synonym: BRADO6426

Alternate gene names: NA

Gene position: 6679304-6680155 (Clockwise)

Preceding gene: 146343214

Following gene: 146343217

Centisome position: 89.58

GC content: 67.25

Gene sequence:

>852_bases
GTGGGCCGGGTCAGGATCACCAAATTCGCCGAGCTGGAAACGGTCGGCGGCACCCGCTTCATCCTGCCGCAGTCGGCCCC
CGAGGCCATCCGCGCGCTGCCCTGGATCACACCGGAGTTCGCCACGGCCGAAGGCAAGCTGAAGATGTCGATCCACACTC
TGGTGCTGGAGACCCCGACGCGGCGCATCGTCGTCGACACCGGCCTCGGCAATGACAAGCAGGGCCGCAGCGTGCCGGTG
TGGAACGGCCGCAGCACGCCGTTCCTGGAGACGATGAGCGCGGCCGGCTTTCCGCCCGAGAGCATCGACATGGTGATCTG
CACGCATCTGCACGTCGACCATGTCGGCTGGAATACGCGGCTGGTCGGCGGCCGCTGGATTCCGACCTTCCCCAACGCAC
GCTACGTGTTCGGCCGCACTGAATATGAGCATTGGCGCGATTACAGCGAGGCTGCCGACGAAACCGCGACCTTCGCCGAT
TCCGTGCAGCCGGTCGTCGCGGCCGGCAGGGCCGAGCTGGTTGCGAGCGACGCTGTCATCAGCGACGAGATCACGCTGAT
CCCGACGCCGGGCCACAGCCCCGGTCATGTCAGCCTGCACATCCGCTCCGGTGGTGCGGAGGCACTGCTGAGCGGCGATG
TCGCACATCATCCCTGCCAGATGGCACATCTGGACTGGAGCTCGACCGTCGACTTCAACCCTGCCCAGGCCATCGCCACC
CGGCAGGCGCTGTTCTCGCGTTTCGCCGATACCGGCACGCTGGTGATCGGCGGCCATTTCGATGCCGGTCAGATCCGCCG
CGACGGCGCGGCGTTCCGCTTTCAGGCAGTGCCGGGCAGCGGCAGCGCATAG

Upstream 100 bases:

>100_bases
AACGCGTGTTCCCGCGACTCGATCCATTGCGCTGCGGCGCGCGACGCGGCATCGTCACTTGCAGCGAAGAAACAAGCGGG
GAGCGCGCATGCAGTGGACG

Downstream 100 bases:

>100_bases
CCCGCAAGGTTGAATTTCGTCCCGCCATGCTCCATGACAGGGCTCTGGAATACCAAGGAACTGAGGGAGAGAAACCAGAT
GAAGCTCGTTCGCTACGGCG

Product: putative metallo-beta-lactamase superfamily protein

Products: substituted beta-amino acid

Alternate protein names: Beta-Lactamase Domain Protein; Metallo-Beta-Lactamase Family Protein; Beta-Lactamase; Metallo-Beta-Lactamase Superfamily Protein; Beta-Lactamase-Like; Twin-Arginine Translocation Pathway Signal; Beta-Lactamase-Like Protein; Metallo-Beta-Lactamase; Zn-Dependent Hydrolase; Hydrolase; Methyl Parathion Hydrolase; Metal-Dependent Hydrolase; Beta-Lactamase Family Protein; Metal Dependent Hydrolase; Metallo-Beta-Lactamase Superfamily; Zn-Dependent Beta-Lactamase Domain Hydrolase; Metallo-Beta-Lactamase Protein; Zinc-Dependent Hydroxylase; Signal Peptide; Beta-Lactamase-Like Zn-Dependent Hydrolase Protein; Metallo-Beta-Lactamase Domain-Containing Protein; MetaL-Dependent Hydrolase; GloB Protein; Aldehyde Dehydrogenase; LOW QUALITY PROTEIN Beta-Lactamase; Signal Peptide Protein; Beta-Lactamase Class B; Zn-Dependent Hydrolase Including Glyoxylase-Like Protein

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGRVRITKFAELETVGGTRFILPQSAPEAIRALPWITPEFATAEGKLKMSIHTLVLETPTRRIVVDTGLGNDKQGRSVPV
WNGRSTPFLETMSAAGFPPESIDMVICTHLHVDHVGWNTRLVGGRWIPTFPNARYVFGRTEYEHWRDYSEAADETATFAD
SVQPVVAAGRAELVASDAVISDEITLIPTPGHSPGHVSLHIRSGGAEALLSGDVAHHPCQMAHLDWSSTVDFNPAQAIAT
RQALFSRFADTGTLVIGGHFDAGQIRRDGAAFRFQAVPGSGSA

Sequences:

>Translated_283_residues
MGRVRITKFAELETVGGTRFILPQSAPEAIRALPWITPEFATAEGKLKMSIHTLVLETPTRRIVVDTGLGNDKQGRSVPV
WNGRSTPFLETMSAAGFPPESIDMVICTHLHVDHVGWNTRLVGGRWIPTFPNARYVFGRTEYEHWRDYSEAADETATFAD
SVQPVVAAGRAELVASDAVISDEITLIPTPGHSPGHVSLHIRSGGAEALLSGDVAHHPCQMAHLDWSSTVDFNPAQAIAT
RQALFSRFADTGTLVIGGHFDAGQIRRDGAAFRFQAVPGSGSA
>Mature_282_residues
GRVRITKFAELETVGGTRFILPQSAPEAIRALPWITPEFATAEGKLKMSIHTLVLETPTRRIVVDTGLGNDKQGRSVPVW
NGRSTPFLETMSAAGFPPESIDMVICTHLHVDHVGWNTRLVGGRWIPTFPNARYVFGRTEYEHWRDYSEAADETATFADS
VQPVVAAGRAELVASDAVISDEITLIPTPGHSPGHVSLHIRSGGAEALLSGDVAHHPCQMAHLDWSSTVDFNPAQAIATR
QALFSRFADTGTLVIGGHFDAGQIRRDGAAFRFQAVPGSGSA

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.2.6

Molecular weight: Translated: 30603; Mature: 30472

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRVRITKFAELETVGGTRFILPQSAPEAIRALPWITPEFATAEGKLKMSIHTLVLETPT
CCCEEEEEEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCC
RRIVVDTGLGNDKQGRSVPVWNGRSTPFLETMSAAGFPPESIDMVICTHLHVDHVGWNTR
CEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEE
LVGGRWIPTFPNARYVFGRTEYEHWRDYSEAADETATFADSVQPVVAAGRAELVASDAVI
EECCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHEEECCCEE
SDEITLIPTPGHSPGHVSLHIRSGGAEALLSGDVAHHPCQMAHLDWSSTVDFNPAQAIAT
CCCEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHH
RQALFSRFADTGTLVIGGHFDAGQIRRDGAAFRFQAVPGSGSA
HHHHHHHHCCCCEEEEECCCCCCCEECCCCEEEEEECCCCCCC
>Mature Secondary Structure 
GRVRITKFAELETVGGTRFILPQSAPEAIRALPWITPEFATAEGKLKMSIHTLVLETPT
CCEEEEEEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCC
RRIVVDTGLGNDKQGRSVPVWNGRSTPFLETMSAAGFPPESIDMVICTHLHVDHVGWNTR
CEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEE
LVGGRWIPTFPNARYVFGRTEYEHWRDYSEAADETATFADSVQPVVAAGRAELVASDAVI
EECCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHEEECCCEE
SDEITLIPTPGHSPGHVSLHIRSGGAEALLSGDVAHHPCQMAHLDWSSTVDFNPAQAIAT
CCCEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHH
RQALFSRFADTGTLVIGGHFDAGQIRRDGAAFRFQAVPGSGSA
HHHHHHHHCCCCEEEEECCCCCCCEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-lactam; H2O

Specific reaction: A beta-lactam + H2O = a substituted beta-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA