BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is braF [H]

Identifier: 146342117

GI number: 146342117

Start: 5472928

End: 5473737

Strand: Direct

Name: braF [H]

Synonym: BRADO5263

Alternate gene names: 146342117

Gene position: 5472928-5473737 (Clockwise)

Preceding gene: 146342113

Following gene: 146342118

Centisome position: 73.4

GC content: 65.31

Gene sequence:

>810_bases
TTGGAAGCGCGCGGAGTCTCGCTGCGTTTCGGCGGGGTGAAAGCCCTGACCGACGTCAGCTTCGGCGTGCGCGAGGGCGA
GCTGTTCTCGATCATCGGCCCGAACGGCGCCGGCAAGACCTCGATCGTGAACTGCATCTCCGGCCGCTATAAGCCGACGG
AAGGCCGCCTGTTCCATCGCGGGCGCGACATCACCGGCCTGCCGCCGAACGCGCGCGCCAATATCGGCATCGGCCGCACC
TTCCAGAACCTCGCGCTGTTCCACCACATGAGCGTGCTCGACAACATCATGGTCGGGCGGCATCATTTGTTGAAGAACAA
CTTCCTCACGGGATCGCTGTACTGGCTCACCGGCGCGCGGCGCGAGGAGCTGAAGCACCGCGAGAAGGTCGAGCAGATCA
TCGACTTCCTCGACCTGCAGTCGGTGCGCAAGGCGCCGGCCGGCACCCTCTCCTACGGCCTGCGCAAGCGCGTCGAGCTC
GCCCGCGCCATGGCGCTGGAGCCGCATCTCATCCTCCTCGACGAGCCGATGGCCGGCATGAACTTCGAGGAGAAGGAGGA
TATGGCCCGCTACATCGTCGATCTCAACGAGGAATACGGCATGACGGTCGTGATGATCGAGCACGACATGGGCGTCGTCA
TGGACATCTCCCACCGCGTGATGGTGCTCGATTTCGGCCGCAAGATCGCCGAGGGCGATCCCGCCGCCGTGCTCGCCGAT
CCGCACGTCAAGCGCGCCTATCTCGGCGAGGAAGACGAACTGCTCGTCGATCCCGACGAGGCGCCGTCCATCGCGGAGGA
CGCGGCATGA

Upstream 100 bases:

>100_bases
GATTGCGGCCAAAGCGACGCGCTGAACGTCGCGAGACACGATGACCGAGAGAGCGCGCCAAGCGCCGAAGACCGTTCGGG
AGGACGACTTGGACTATACG

Downstream 100 bases:

>100_bases
TGGACTATGCTCGCCGCGTGACGCAGGCCGACACCTATCCAAAGCTGCTGCGCCTGAACGCCAAAGAGCACGGCCAGGAG
ATTGCGCTGCGCGAGAAGGA

Product: putative high-affinity branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MEARGVSLRFGGVKALTDVSFGVREGELFSIIGPNGAGKTSIVNCISGRYKPTEGRLFHRGRDITGLPPNARANIGIGRT
FQNLALFHHMSVLDNIMVGRHHLLKNNFLTGSLYWLTGARREELKHREKVEQIIDFLDLQSVRKAPAGTLSYGLRKRVEL
ARAMALEPHLILLDEPMAGMNFEEKEDMARYIVDLNEEYGMTVVMIEHDMGVVMDISHRVMVLDFGRKIAEGDPAAVLAD
PHVKRAYLGEEDELLVDPDEAPSIAEDAA

Sequences:

>Translated_269_residues
MEARGVSLRFGGVKALTDVSFGVREGELFSIIGPNGAGKTSIVNCISGRYKPTEGRLFHRGRDITGLPPNARANIGIGRT
FQNLALFHHMSVLDNIMVGRHHLLKNNFLTGSLYWLTGARREELKHREKVEQIIDFLDLQSVRKAPAGTLSYGLRKRVEL
ARAMALEPHLILLDEPMAGMNFEEKEDMARYIVDLNEEYGMTVVMIEHDMGVVMDISHRVMVLDFGRKIAEGDPAAVLAD
PHVKRAYLGEEDELLVDPDEAPSIAEDAA
>Mature_269_residues
MEARGVSLRFGGVKALTDVSFGVREGELFSIIGPNGAGKTSIVNCISGRYKPTEGRLFHRGRDITGLPPNARANIGIGRT
FQNLALFHHMSVLDNIMVGRHHLLKNNFLTGSLYWLTGARREELKHREKVEQIIDFLDLQSVRKAPAGTLSYGLRKRVEL
ARAMALEPHLILLDEPMAGMNFEEKEDMARYIVDLNEEYGMTVVMIEHDMGVVMDISHRVMVLDFGRKIAEGDPAAVLAD
PHVKRAYLGEEDELLVDPDEAPSIAEDAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI45446740, Length=221, Percent_Identity=29.8642533936652, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI47078218, Length=221, Percent_Identity=29.8642533936652, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27881501, Length=267, Percent_Identity=24.7191011235955, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI30795238, Length=267, Percent_Identity=24.7191011235955, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI105990541, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI31657092, Length=230, Percent_Identity=26.5217391304348, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI153792144, Length=173, Percent_Identity=30.0578034682081, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI116734710, Length=198, Percent_Identity=26.2626262626263, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI42490749, Length=243, Percent_Identity=27.1604938271605, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI156105685, Length=217, Percent_Identity=27.1889400921659, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI42741659, Length=237, Percent_Identity=25.7383966244726, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI27262624, Length=175, Percent_Identity=26.8571428571429, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI27262626, Length=175, Percent_Identity=26.8571428571429, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI171184400, Length=241, Percent_Identity=24.896265560166, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI21536376, Length=226, Percent_Identity=25.2212389380531, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI46592964, Length=232, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592956, Length=232, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592915, Length=220, Percent_Identity=25.4545454545455, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592898, Length=220, Percent_Identity=25.4545454545455, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592978, Length=232, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI46592971, Length=232, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI150417984, Length=220, Percent_Identity=25.4545454545455, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=37.6, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI87081791, Length=244, Percent_Identity=31.1475409836066, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789593, Length=251, Percent_Identity=32.6693227091633, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788761, Length=256, Percent_Identity=28.90625, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787712, Length=227, Percent_Identity=31.7180616740088, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI87081709, Length=241, Percent_Identity=29.8755186721992, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787029, Length=246, Percent_Identity=28.4552845528455, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790467, Length=234, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789032, Length=244, Percent_Identity=31.1475409836066, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790190, Length=244, Percent_Identity=28.6885245901639, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=26.1603375527426, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788210, Length=228, Percent_Identity=31.140350877193, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786398, Length=236, Percent_Identity=27.1186440677966, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48995001, Length=229, Percent_Identity=30.5676855895196, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788472, Length=240, Percent_Identity=28.75, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786872, Length=245, Percent_Identity=28.1632653061224, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788225, Length=255, Percent_Identity=28.2352941176471, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082267, Length=231, Percent_Identity=28.1385281385281, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786563, Length=247, Percent_Identity=28.3400809716599, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082268, Length=245, Percent_Identity=27.7551020408163, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786253, Length=230, Percent_Identity=29.5652173913043, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1786319, Length=229, Percent_Identity=29.2576419213974, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87081782, Length=256, Percent_Identity=26.953125, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1789672, Length=253, Percent_Identity=24.901185770751, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789991, Length=242, Percent_Identity=26.8595041322314, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790525, Length=247, Percent_Identity=27.5303643724696, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789962, Length=233, Percent_Identity=28.3261802575107, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787089, Length=254, Percent_Identity=27.5590551181102, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788450, Length=242, Percent_Identity=25.6198347107438, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI48994997, Length=229, Percent_Identity=27.0742358078603, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=26.9709543568465, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786345, Length=235, Percent_Identity=25.531914893617, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789873, Length=221, Percent_Identity=29.4117647058824, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788644, Length=249, Percent_Identity=30.9236947791165, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI48994943, Length=283, Percent_Identity=25.0883392226148, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789586, Length=246, Percent_Identity=29.6747967479675, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790739, Length=262, Percent_Identity=26.3358778625954, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787500, Length=234, Percent_Identity=27.3504273504274, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788053, Length=227, Percent_Identity=26.8722466960352, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787758, Length=236, Percent_Identity=25.4237288135593, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789891, Length=213, Percent_Identity=26.7605633802817, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786803, Length=244, Percent_Identity=24.5901639344262, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081834, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693107, Length=242, Percent_Identity=26.4462809917355, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787792, Length=268, Percent_Identity=25, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788506, Length=236, Percent_Identity=27.5423728813559, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788897, Length=223, Percent_Identity=24.6636771300448, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787164, Length=212, Percent_Identity=25, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994883, Length=243, Percent_Identity=25.5144032921811, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786981, Length=211, Percent_Identity=28.436018957346, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786703, Length=198, Percent_Identity=25.2525252525253, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789963, Length=254, Percent_Identity=22.8346456692913, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787041, Length=241, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787548, Length=257, Percent_Identity=25.6809338521401, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787105, Length=200, Percent_Identity=25, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787972, Length=182, Percent_Identity=29.1208791208791, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI115534520, Length=238, Percent_Identity=25.2100840336134, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=221, Percent_Identity=25.3393665158371, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=221, Percent_Identity=25.3393665158371, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=221, Percent_Identity=25.3393665158371, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=220, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212646699, Length=232, Percent_Identity=22.8448275862069, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17541710, Length=231, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24666092, Length=227, Percent_Identity=26.8722466960352, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221512771, Length=225, Percent_Identity=29.7777777777778, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648314, Length=232, Percent_Identity=25.8620689655172, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650853, Length=230, Percent_Identity=24.3478260869565, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650855, Length=230, Percent_Identity=24.7826086956522, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362751, Length=230, Percent_Identity=24.3478260869565, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643674, Length=234, Percent_Identity=24.7863247863248, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571209, Length=233, Percent_Identity=24.4635193133047, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24582014, Length=249, Percent_Identity=24.8995983935743, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582012, Length=249, Percent_Identity=24.8995983935743, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29918; Mature: 29918

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARGVSLRFGGVKALTDVSFGVREGELFSIIGPNGAGKTSIVNCISGRYKPTEGRLFHR
CCCCCEEEEECCCCEEHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEC
GRDITGLPPNARANIGIGRTFQNLALFHHMSVLDNIMVGRHHLLKNNFLTGSLYWLTGAR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
REELKHREKVEQIIDFLDLQSVRKAPAGTLSYGLRKRVELARAMALEPHLILLDEPMAGM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
NFEEKEDMARYIVDLNEEYGMTVVMIEHDMGVVMDISHRVMVLDFGRKIAEGDPAAVLAD
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCEEEEEECCCHHCCCCCCEEEEC
PHVKRAYLGEEDELLVDPDEAPSIAEDAA
CCHHHHHCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MEARGVSLRFGGVKALTDVSFGVREGELFSIIGPNGAGKTSIVNCISGRYKPTEGRLFHR
CCCCCEEEEECCCCEEHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEC
GRDITGLPPNARANIGIGRTFQNLALFHHMSVLDNIMVGRHHLLKNNFLTGSLYWLTGAR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
REELKHREKVEQIIDFLDLQSVRKAPAGTLSYGLRKRVELARAMALEPHLILLDEPMAGM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
NFEEKEDMARYIVDLNEEYGMTVVMIEHDMGVVMDISHRVMVLDFGRKIAEGDPAAVLAD
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCEEEEEECCCHHCCCCCCEEEEC
PHVKRAYLGEEDELLVDPDEAPSIAEDAA
CCHHHHHCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]