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The map label for this gene is fcl [H]

Identifier: 146342114

GI number: 146342114

Start: 5469762

End: 5470658

Strand: Reverse

Name: fcl [H]

Synonym: BRADO5259

Alternate gene names: 146342114

Gene position: 5470658-5469762 (Counterclockwise)

Preceding gene: 146342115

Following gene: 146342112

Centisome position: 73.37

GC content: 66.44

Gene sequence:

>897_bases
ATGGTCGGCAGCGCGCTGGTGCGGCGCTTGGCAAGCGAGGGCGTCGAACTGCAGACCGTGTCGCGCGGCGAGGTCGATCT
GCGCGACCAGGCTGCAGTGTTCTCCTGGTTCGCGCGCGCGAAGCCCCAGGTCGTCTTCCTCGCGGCCGCCAAGGTCGGCG
GCATCGTCGCCAACAACACGCTCCGTGCCGAGTTCATCTACGACAACATCGCCATCGCCACCAACGTCATCCACGCCGCG
CATGTGAACGGCGCCGAGAAGCTGATGTTTCTCGGCTCGTCCTGCATCTATCCCAAGCTCGCTCCGCAGCCGCTGCGCGA
GGATGCGATGCTTACAGGGCCGCTGGAGTCGACCAACGAGCCCTATGCGATCGCCAAGATCGCCGGCATCAAGATGGTCG
AGGCCTATCGCAGCCAGTATGACGCCGACTTCATCAACGTCATGCCGACCAACCTCTACGGCCCCGGCGACAACTATCAC
CCCGAATACAGCCACGTCGTCGCGGCGCTGATCCGCCGCTTCCATGAGGCGAAGCTTGCGGACGCGCCAGAGGTCGTGGT
CTGGGGCACCGGCACGCCGCGGCGCGAATTCCTCTATGTCGACGACATGGCGGACGCCTGCGTGCACCTGATGAAGACCT
ACTCGGATCACGAGCTGGTCAACATCGGCACCGGCGAGGACATCACCATTGCCGACTTTGCCCGCGTGGTGGCCGCAACG
GTCGGCTATCAGGGGCGCCTCAGCTTCGATCCCTCGCGCCCCGACGGCACGCCGCGCAAGCTGCTCGACGTCTCGCGGCT
CGCCAAGCTCGGCTGGCGCGCGACGACGTCGCTGGAGGATGGCATCGCGCGCGCCTATCAGGCGTTCCTGCGCGAGACCG
GGCAGGCCGCCGCGTAA

Upstream 100 bases:

>100_bases
ATGGTGGCGAGCGATCTCAGCACGGCGCGGCAGGAGACGACCCGTGGCCACGCCTTTTGATCTGCATGGCAAGCGGGTCT
TCGTCGCCGGCTATCGCGGC

Downstream 100 bases:

>100_bases
CTCTCCGCCGTTGTCCCGGGTCAAGCCCGGGACGACACCGTTAGTGAGGCGCCATTCGCTGTGCTTTCACAGCAAGCGCA
GTAGTCATGGCGAGATCAGC

Product: GDP-L-fucose synthase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MVGSALVRRLASEGVELQTVSRGEVDLRDQAAVFSWFARAKPQVVFLAAAKVGGIVANNTLRAEFIYDNIAIATNVIHAA
HVNGAEKLMFLGSSCIYPKLAPQPLREDAMLTGPLESTNEPYAIAKIAGIKMVEAYRSQYDADFINVMPTNLYGPGDNYH
PEYSHVVAALIRRFHEAKLADAPEVVVWGTGTPRREFLYVDDMADACVHLMKTYSDHELVNIGTGEDITIADFARVVAAT
VGYQGRLSFDPSRPDGTPRKLLDVSRLAKLGWRATTSLEDGIARAYQAFLRETGQAAA

Sequences:

>Translated_298_residues
MVGSALVRRLASEGVELQTVSRGEVDLRDQAAVFSWFARAKPQVVFLAAAKVGGIVANNTLRAEFIYDNIAIATNVIHAA
HVNGAEKLMFLGSSCIYPKLAPQPLREDAMLTGPLESTNEPYAIAKIAGIKMVEAYRSQYDADFINVMPTNLYGPGDNYH
PEYSHVVAALIRRFHEAKLADAPEVVVWGTGTPRREFLYVDDMADACVHLMKTYSDHELVNIGTGEDITIADFARVVAAT
VGYQGRLSFDPSRPDGTPRKLLDVSRLAKLGWRATTSLEDGIARAYQAFLRETGQAAA
>Mature_298_residues
MVGSALVRRLASEGVELQTVSRGEVDLRDQAAVFSWFARAKPQVVFLAAAKVGGIVANNTLRAEFIYDNIAIATNVIHAA
HVNGAEKLMFLGSSCIYPKLAPQPLREDAMLTGPLESTNEPYAIAKIAGIKMVEAYRSQYDADFINVMPTNLYGPGDNYH
PEYSHVVAALIRRFHEAKLADAPEVVVWGTGTPRREFLYVDDMADACVHLMKTYSDHELVNIGTGEDITIADFARVVAAT
VGYQGRLSFDPSRPDGTPRKLLDVSRLAKLGWRATTSLEDGIARAYQAFLRETGQAAA

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=273, Percent_Identity=32.2344322344322, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI7657641, Length=272, Percent_Identity=24.2647058823529, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=302, Percent_Identity=58.6092715231788, Blast_Score=355, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17554428, Length=274, Percent_Identity=28.4671532846715, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532424, Length=281, Percent_Identity=25.9786476868327, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=284, Percent_Identity=25, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922778, Length=308, Percent_Identity=31.8181818181818, Blast_Score=154, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 32560; Mature: 32560

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGSALVRRLASEGVELQTVSRGEVDLRDQAAVFSWFARAKPQVVFLAAAKVGGIVANNT
CCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHCCEEECCE
LRAEFIYDNIAIATNVIHAAHVNGAEKLMFLGSSCIYPKLAPQPLREDAMLTGPLESTNE
EEEEEEECCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
PYAIAKIAGIKMVEAYRSQYDADFINVMPTNLYGPGDNYHPEYSHVVAALIRRFHEAKLA
CEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DAPEVVVWGTGTPRREFLYVDDMADACVHLMKTYSDHELVNIGTGEDITIADFARVVAAT
CCCCEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHH
VGYQGRLSFDPSRPDGTPRKLLDVSRLAKLGWRATTSLEDGIARAYQAFLRETGQAAA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVGSALVRRLASEGVELQTVSRGEVDLRDQAAVFSWFARAKPQVVFLAAAKVGGIVANNT
CCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHCCEEECCE
LRAEFIYDNIAIATNVIHAAHVNGAEKLMFLGSSCIYPKLAPQPLREDAMLTGPLESTNE
EEEEEEECCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
PYAIAKIAGIKMVEAYRSQYDADFINVMPTNLYGPGDNYHPEYSHVVAALIRRFHEAKLA
CEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DAPEVVVWGTGTPRREFLYVDDMADACVHLMKTYSDHELVNIGTGEDITIADFARVVAAT
CCCCEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHH
VGYQGRLSFDPSRPDGTPRKLLDVSRLAKLGWRATTSLEDGIARAYQAFLRETGQAAA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]