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The map label for this gene is purU [H]

Identifier: 146342112

GI number: 146342112

Start: 5467654

End: 5468517

Strand: Reverse

Name: purU [H]

Synonym: BRADO5257

Alternate gene names: 146342112

Gene position: 5468517-5467654 (Counterclockwise)

Preceding gene: 146342114

Following gene: 146342110

Centisome position: 73.34

GC content: 63.19

Gene sequence:

>864_bases
ATGTCCGACCACCAATATGTTCTGACCCTGTCCTGCCCGGATCGTCCTGGGATCGTCTCAGCCGTCTCTACCTTCCTTGC
CCATAACGGCCAGAACATCCTCGATGCCCAGCAGTTCGGCGACTTCGAGACCGGTCACTTTTTCATGCGCGTGGTGTTCA
CGGCTGCCGATCTCGCGGTCAACCTGCCGGCGCTGCAGACCGGCTTCGCGGCGATCGCCGAGCGCTTTGCGATGGATTGG
CAGATGCGCGACCGCGCCGGCCAACGTCGTGTGATGCTCTTGGTCTCGAAGTTCGACCATTGCCTCGTCGACATCCTCTA
TCGCTGGCGCACCCGCGACCTGTCGATGATCCCGACTGCGATCGTCTCCAACCACCCGCGGGAGACCTATGCCGGGCTGG
ATTTGGGCGAGATCCCGTTTCACCACATGCCGGTCACCAAGGAGACCAAGCGCGACCAGGAGCAGGCGATCCTCAAGCTG
GTCGACGACACCAAGACCGATCTCGTGGTGCTGGCGCGCTACATGCAGATCCTGTCGGACGAGATGTCGGCCAGCCTGTC
CGGCCGCTGCATCAACATCCACCACTCGTTCCTGCCGGGCTTCAAGGGCGCCAAACCCTACCACCAGGCCTATGAGCGCG
GCGTCAAGCTGATCGGCGCCACCGCCCATTACGTCACCCGCGACCTCGACGAGGGCCCGATCATCGACCAGGACGTCGAG
CGGATCAGCCATCGCGACACGCCCGAGGATCTCGTGCGCAAGGGCCGCGACATCGAGCGCCGCGTGCTGGCGCGTGCGAT
TCGTTATCACCTCGAGGATCGCGTCATCCTGAACGGCCGCAAGACCGTCGTGTTCATGGATTGA

Upstream 100 bases:

>100_bases
CCGAGCGTTGGCGCAAGGGCGCGGAGGGTGATAGAGCAGGGCATGGAACGGCCGTCAGGCCCGCCGCCAGATCACTTTTC
CCATTCGGTCCCTCATCGCC

Downstream 100 bases:

>100_bases
TTGTTCAGCGAGTGGGGGCAGCCGCCTGCGCATTGGCGTCGGACTGCGTCTCCTTGTCGCGTTCGGCGGCCGGCAGCTGC
CTCTGCCGCTCGCGCTCTTG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSDHQYVLTLSCPDRPGIVSAVSTFLAHNGQNILDAQQFGDFETGHFFMRVVFTAADLAVNLPALQTGFAAIAERFAMDW
QMRDRAGQRRVMLLVSKFDHCLVDILYRWRTRDLSMIPTAIVSNHPRETYAGLDLGEIPFHHMPVTKETKRDQEQAILKL
VDDTKTDLVVLARYMQILSDEMSASLSGRCINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTRDLDEGPIIDQDVE
RISHRDTPEDLVRKGRDIERRVLARAIRYHLEDRVILNGRKTVVFMD

Sequences:

>Translated_287_residues
MSDHQYVLTLSCPDRPGIVSAVSTFLAHNGQNILDAQQFGDFETGHFFMRVVFTAADLAVNLPALQTGFAAIAERFAMDW
QMRDRAGQRRVMLLVSKFDHCLVDILYRWRTRDLSMIPTAIVSNHPRETYAGLDLGEIPFHHMPVTKETKRDQEQAILKL
VDDTKTDLVVLARYMQILSDEMSASLSGRCINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTRDLDEGPIIDQDVE
RISHRDTPEDLVRKGRDIERRVLARAIRYHLEDRVILNGRKTVVFMD
>Mature_286_residues
SDHQYVLTLSCPDRPGIVSAVSTFLAHNGQNILDAQQFGDFETGHFFMRVVFTAADLAVNLPALQTGFAAIAERFAMDWQ
MRDRAGQRRVMLLVSKFDHCLVDILYRWRTRDLSMIPTAIVSNHPRETYAGLDLGEIPFHHMPVTKETKRDQEQAILKLV
DDTKTDLVVLARYMQILSDEMSASLSGRCINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTRDLDEGPIIDQDVER
ISHRDTPEDLVRKGRDIERRVLARAIRYHLEDRVILNGRKTVVFMD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=30.8457711442786, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=30.8457711442786, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=30.8457711442786, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=40.3508771929825, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788846, Length=128, Percent_Identity=29.6875, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=169, Percent_Identity=34.9112426035503, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32773; Mature: 32642

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHQYVLTLSCPDRPGIVSAVSTFLAHNGQNILDAQQFGDFETGHFFMRVVFTAADLAV
CCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHC
NLPALQTGFAAIAERFAMDWQMRDRAGQRRVMLLVSKFDHCLVDILYRWRTRDLSMIPTA
CCCHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IVSNHPRETYAGLDLGEIPFHHMPVTKETKRDQEQAILKLVDDTKTDLVVLARYMQILSD
HHCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EMSASLSGRCINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTRDLDEGPIIDQDVE
HHHHCCCCCEEEEEHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
RISHRDTPEDLVRKGRDIERRVLARAIRYHLEDRVILNGRKTVVFMD
HHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEC
>Mature Secondary Structure 
SDHQYVLTLSCPDRPGIVSAVSTFLAHNGQNILDAQQFGDFETGHFFMRVVFTAADLAV
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHC
NLPALQTGFAAIAERFAMDWQMRDRAGQRRVMLLVSKFDHCLVDILYRWRTRDLSMIPTA
CCCHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IVSNHPRETYAGLDLGEIPFHHMPVTKETKRDQEQAILKLVDDTKTDLVVLARYMQILSD
HHCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EMSASLSGRCINIHHSFLPGFKGAKPYHQAYERGVKLIGATAHYVTRDLDEGPIIDQDVE
HHHHCCCCCEEEEEHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
RISHRDTPEDLVRKGRDIERRVLARAIRYHLEDRVILNGRKTVVFMD
HHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]