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The map label for this gene is gmd [H]

Identifier: 146342115

GI number: 146342115

Start: 5470699

End: 5471754

Strand: Reverse

Name: gmd [H]

Synonym: BRADO5260

Alternate gene names: 146342115

Gene position: 5471754-5470699 (Counterclockwise)

Preceding gene: 146342116

Following gene: 146342114

Centisome position: 73.38

GC content: 63.73

Gene sequence:

>1056_bases
GTGGCTCTGATCACCGGTGTGACGGGACAGGACGGCGCCTATCTCGCCGAATATCTGCTCGGCCTCGGCTATACCGTGCA
CGGCGTCAAGCGACGTTCGTCGTCCTTCAATACCGCGCGCGTCGATCATCTCTACCGCGACCCGCACGAAGACAACGTGC
CGTTCCTGCTGCATTACGGCGACATGACCGATTCCACCAACCTGATCCGGCTGGTGCAGCAGATCCGCCCCGACGAGATC
TACAATCTCGCCGCCCAGAGCCATGTCGGGGTCAGCTTCGAGAGCCCGGAATACACCGCCAATGCCGACGGGCTCGGCGT
GCTCAGGCTGTTGGAAGCCATTCGCATTCTCGGCATGGAGAAGCAGACGCGATTCTACCAGGCCTCGACCTCGGAGCTGT
ATGGCCTGGTCCAGGAGATCCCGCAGCGCGAGACCACGCCGTTCTATCCGCGCTCGCCCTATGGCTGCGCCAAGCTCTAC
GGCTACTGGATTACGGTCAACTATCGCGAAGCCTACGGCATGTTCGCCTGTAACGGCATCCTGTTCAACCATGAGAGCCC
GATCCGCGGTGAGACCTTCGTCACGCGGAAGATCACCCGGGGCGTCGCCCGGATCGAGGCCGGCCTGGAGGAGACGATCT
ATCTCGGCAATCTCGAGGCCAAGCGCGACTGGGGCCACGCCCGCGACTATGTCGAAGGCATGCATCGCATTCTCCAGGCC
GATGCGCCGGACGATTACGTGCTGGCCACCGGCGAGACCCATTCGGTGCGCGAGCTGGTCGAGCTCGCCTTTGCCGAGGT
CGATCGCCGCATCTCATGGCGCGGCGAGGGCGTCGAGGAGACCGGCATCGATTGCAGATCCGGCAAGACAGTGGTGCGCA
TCGACCCGACCTATTTCCGGCCGACAGAGGTCGATCTGCTGGTCGGCGACTGCAGCAAGGCGCGCGACAAGCTGGGCTGG
ACGCCGAAGACGAGCTTCGCGCAACTGGTGAAGGAGATGGTGGCGAGCGATCTCAGCACGGCGCGGCAGGAGACGACCCG
TGGCCACGCCTTTTGA

Upstream 100 bases:

>100_bases
CGCTCGTCCCGGCTATGAACTGGTCACAGGACCCCTCGGCTCTGACGGCCGGGGGCAGTGTGGTGGAGACGGTATGACGG
AGCAGAATTTCAGCGGCCGC

Downstream 100 bases:

>100_bases
TCTGCATGGCAAGCGGGTCTTCGTCGCCGGCTATCGCGGCATGGTCGGCAGCGCGCTGGTGCGGCGCTTGGCAAGCGAGG
GCGTCGAACTGCAGACCGTG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTARVDHLYRDPHEDNVPFLLHYGDMTDSTNLIRLVQQIRPDEI
YNLAAQSHVGVSFESPEYTANADGLGVLRLLEAIRILGMEKQTRFYQASTSELYGLVQEIPQRETTPFYPRSPYGCAKLY
GYWITVNYREAYGMFACNGILFNHESPIRGETFVTRKITRGVARIEAGLEETIYLGNLEAKRDWGHARDYVEGMHRILQA
DAPDDYVLATGETHSVRELVELAFAEVDRRISWRGEGVEETGIDCRSGKTVVRIDPTYFRPTEVDLLVGDCSKARDKLGW
TPKTSFAQLVKEMVASDLSTARQETTRGHAF

Sequences:

>Translated_351_residues
MALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTARVDHLYRDPHEDNVPFLLHYGDMTDSTNLIRLVQQIRPDEI
YNLAAQSHVGVSFESPEYTANADGLGVLRLLEAIRILGMEKQTRFYQASTSELYGLVQEIPQRETTPFYPRSPYGCAKLY
GYWITVNYREAYGMFACNGILFNHESPIRGETFVTRKITRGVARIEAGLEETIYLGNLEAKRDWGHARDYVEGMHRILQA
DAPDDYVLATGETHSVRELVELAFAEVDRRISWRGEGVEETGIDCRSGKTVVRIDPTYFRPTEVDLLVGDCSKARDKLGW
TPKTSFAQLVKEMVASDLSTARQETTRGHAF
>Mature_350_residues
ALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTARVDHLYRDPHEDNVPFLLHYGDMTDSTNLIRLVQQIRPDEIY
NLAAQSHVGVSFESPEYTANADGLGVLRLLEAIRILGMEKQTRFYQASTSELYGLVQEIPQRETTPFYPRSPYGCAKLYG
YWITVNYREAYGMFACNGILFNHESPIRGETFVTRKITRGVARIEAGLEETIYLGNLEAKRDWGHARDYVEGMHRILQAD
APDDYVLATGETHSVRELVELAFAEVDRRISWRGEGVEETGIDCRSGKTVVRIDPTYFRPTEVDLLVGDCSKARDKLGWT
PKTSFAQLVKEMVASDLSTARQETTRGHAF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=60.5797101449275, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1788366, Length=355, Percent_Identity=62.2535211267606, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=26.5129682997118, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=308, Percent_Identity=24.3506493506494, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=64.7058823529412, Blast_Score=465, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=64.7058823529412, Blast_Score=465, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=64.7058823529412, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=64.7058823529412, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=64.7058823529412, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=64.8093841642229, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24158427, Length=344, Percent_Identity=62.7906976744186, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39579; Mature: 39448

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTARVDHLYRDPHEDNVPFLLHYG
CEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEC
DMTDSTNLIRLVQQIRPDEIYNLAAQSHVGVSFESPEYTANADGLGVLRLLEAIRILGME
CCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCC
KQTRFYQASTSELYGLVQEIPQRETTPFYPRSPYGCAKLYGYWITVNYREAYGMFACNGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEEEEEEHHHCCEEEECEE
LFNHESPIRGETFVTRKITRGVARIEAGLEETIYLGNLEAKRDWGHARDYVEGMHRILQA
EECCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHCCCHHHHHHHHHHHHHHC
DAPDDYVLATGETHSVRELVELAFAEVDRRISWRGEGVEETGIDCRSGKTVVRIDPTYFR
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCEEEEECCCEEC
PTEVDLLVGDCSKARDKLGWTPKTSFAQLVKEMVASDLSTARQETTRGHAF
CCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTARVDHLYRDPHEDNVPFLLHYG
EEEECCCCCCHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEC
DMTDSTNLIRLVQQIRPDEIYNLAAQSHVGVSFESPEYTANADGLGVLRLLEAIRILGME
CCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCC
KQTRFYQASTSELYGLVQEIPQRETTPFYPRSPYGCAKLYGYWITVNYREAYGMFACNGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEEEEEEHHHCCEEEECEE
LFNHESPIRGETFVTRKITRGVARIEAGLEETIYLGNLEAKRDWGHARDYVEGMHRILQA
EECCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHCCCHHHHHHHHHHHHHHC
DAPDDYVLATGETHSVRELVELAFAEVDRRISWRGEGVEETGIDCRSGKTVVRIDPTYFR
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCEEEEECCCEEC
PTEVDLLVGDCSKARDKLGWTPKTSFAQLVKEMVASDLSTARQETTRGHAF
CCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]