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The map label for this gene is rfaD [H]

Identifier: 146342062

GI number: 146342062

Start: 5415227

End: 5416207

Strand: Reverse

Name: rfaD [H]

Synonym: BRADO5202

Alternate gene names: 146342062

Gene position: 5416207-5415227 (Counterclockwise)

Preceding gene: 146342066

Following gene: 146342061

Centisome position: 72.64

GC content: 65.44

Gene sequence:

>981_bases
ATGTTGCTGGTGACCGGCGGCGCCGGGTTTATCGGATCGAATCTGGTCGCGGCGCTGAACGACGCCGGCCGTACCGATGT
CGTCGTCTGCGACACCCTCGGCAGCGAGGGCAAGTGGCGCAATCTGGCCAAACGTCAGCTCGCCGATGTCGTGCCGCCGT
CCGACCTGGACGCCTGGCTGAAGGGCCGTCGTCTCGACGCCGTCCTGCACATGGGCGCGATCTCCGCGACCACCGTGGTC
GACGGCGACCTCGTGATGGAGCGCAATTTCCGCAGTTCCCTGCGTCTGCTCGACTGGTGCACGGCCAACACCACGCCGTT
CATCTACGCCTCCTCGGCTGCGACCTATGGCGACGGCGCGCAGGGCTTCGTGGATGATCCGTCGCTCGCGGCGCTGAAGA
CGCTCCGGCCGATGAACCTCTACGGCTGGAGCAAGCATCTGTTCGACACGGCGATCGCGGCGCGCGCCGAGCGCGGCGAG
CGGCTGCCGCCGCAATACGCTGGCTTGAAGTTCTTCAACGTGTTCGGTCCCAACGAGTACCACAAGGGATCGATGATGAG
CGTATTGACGCGCCGCTTCGACGACATCAAGGCCGGGCACACTTTGCAGCTGTTCAAGTCGCACCGGGACGGCATTGCCG
ACGGTGACCAGCGCCGCGACTTCATCTATGTCGACGACGTCGTGCGCGTGATGCTGTGGCTGCTGGCGACGCCATCCGTC
TCCGGGCTGTTCAACGTCGGCACGGGCCAGGCGCGCAGCTTCAAGGACATGATGCTGGCGGTCTACGCCACGCTTGGCGC
TGTGCCGCGGATCGACTACGTCGACATGCCCGAAGCGATTCGCGGCAGCTACCAGTACTTCACCCAGAGCAATGTCGATC
GCCTGCTGGCCGCCGGCTACAACGGCGGCTTCACGCCGCTCGAGGATGCCGTGGCCGCCTATGTCCGAGACTATCTCGAT
TCCGCCGACCGCTTCCGCTAG

Upstream 100 bases:

>100_bases
GGTAAAGTGGCCGGTCTCGGCCGTCACCAAAGCGCTTACACTTTGTCGCCGCATGCGCTACGCGAGGGCCGGGTTTTCGG
GATCAGGACGGGCTAACGGA

Downstream 100 bases:

>100_bases
ACGAACTGTTCTGGATCACCTACCGAGACTGCCGCTGATGTTCGACTTTGACGATCTCTCGCAAGCGATCGCCCGCCAGA
CGGTGCTGTGCGTCGGCGAC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLLVTGGAGFIGSNLVAALNDAGRTDVVVCDTLGSEGKWRNLAKRQLADVVPPSDLDAWLKGRRLDAVLHMGAISATTVV
DGDLVMERNFRSSLRLLDWCTANTTPFIYASSAATYGDGAQGFVDDPSLAALKTLRPMNLYGWSKHLFDTAIAARAERGE
RLPPQYAGLKFFNVFGPNEYHKGSMMSVLTRRFDDIKAGHTLQLFKSHRDGIADGDQRRDFIYVDDVVRVMLWLLATPSV
SGLFNVGTGQARSFKDMMLAVYATLGAVPRIDYVDMPEAIRGSYQYFTQSNVDRLLAAGYNGGFTPLEDAVAAYVRDYLD
SADRFR

Sequences:

>Translated_326_residues
MLLVTGGAGFIGSNLVAALNDAGRTDVVVCDTLGSEGKWRNLAKRQLADVVPPSDLDAWLKGRRLDAVLHMGAISATTVV
DGDLVMERNFRSSLRLLDWCTANTTPFIYASSAATYGDGAQGFVDDPSLAALKTLRPMNLYGWSKHLFDTAIAARAERGE
RLPPQYAGLKFFNVFGPNEYHKGSMMSVLTRRFDDIKAGHTLQLFKSHRDGIADGDQRRDFIYVDDVVRVMLWLLATPSV
SGLFNVGTGQARSFKDMMLAVYATLGAVPRIDYVDMPEAIRGSYQYFTQSNVDRLLAAGYNGGFTPLEDAVAAYVRDYLD
SADRFR
>Mature_326_residues
MLLVTGGAGFIGSNLVAALNDAGRTDVVVCDTLGSEGKWRNLAKRQLADVVPPSDLDAWLKGRRLDAVLHMGAISATTVV
DGDLVMERNFRSSLRLLDWCTANTTPFIYASSAATYGDGAQGFVDDPSLAALKTLRPMNLYGWSKHLFDTAIAARAERGE
RLPPQYAGLKFFNVFGPNEYHKGSMMSVLTRRFDDIKAGHTLQLFKSHRDGIADGDQRRDFIYVDDVVRVMLWLLATPSV
SGLFNVGTGQARSFKDMMLAVYATLGAVPRIDYVDMPEAIRGSYQYFTQSNVDRLLAAGYNGGFTPLEDAVAAYVRDYLD
SADRFR

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=325, Percent_Identity=39.6923076923077, Blast_Score=217, Evalue=9e-58,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 35862; Mature: 35862

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVTGGAGFIGSNLVAALNDAGRTDVVVCDTLGSEGKWRNLAKRQLADVVPPSDLDAWL
CEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
KGRRLDAVLHMGAISATTVVDGDLVMERNFRSSLRLLDWCTANTTPFIYASSAATYGDGA
CCCHHHHHHHHCCCCEEEEECCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
QGFVDDPSLAALKTLRPMNLYGWSKHLFDTAIAARAERGERLPPQYAGLKFFNVFGPNEY
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCC
HKGSMMSVLTRRFDDIKAGHTLQLFKSHRDGIADGDQRRDFIYVDDVVRVMLWLLATPSV
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCC
SGLFNVGTGQARSFKDMMLAVYATLGAVPRIDYVDMPEAIRGSYQYFTQSNVDRLLAAGY
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHCCHHHHHHCCC
NGGFTPLEDAVAAYVRDYLDSADRFR
CCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLVTGGAGFIGSNLVAALNDAGRTDVVVCDTLGSEGKWRNLAKRQLADVVPPSDLDAWL
CEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
KGRRLDAVLHMGAISATTVVDGDLVMERNFRSSLRLLDWCTANTTPFIYASSAATYGDGA
CCCHHHHHHHHCCCCEEEEECCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
QGFVDDPSLAALKTLRPMNLYGWSKHLFDTAIAARAERGERLPPQYAGLKFFNVFGPNEY
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCC
HKGSMMSVLTRRFDDIKAGHTLQLFKSHRDGIADGDQRRDFIYVDDVVRVMLWLLATPSV
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCC
SGLFNVGTGQARSFKDMMLAVYATLGAVPRIDYVDMPEAIRGSYQYFTQSNVDRLLAAGY
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHCCHHHHHHCCC
NGGFTPLEDAVAAYVRDYLDSADRFR
CCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA