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The map label for this gene is rfaE [H]

Identifier: 146342061

GI number: 146342061

Start: 5413717

End: 5415189

Strand: Reverse

Name: rfaE [H]

Synonym: BRADO5201

Alternate gene names: 146342061

Gene position: 5415189-5413717 (Counterclockwise)

Preceding gene: 146342062

Following gene: 146342060

Centisome position: 72.62

GC content: 67.55

Gene sequence:

>1473_bases
ATGTTCGACTTTGACGATCTCTCGCAAGCGATCGCCCGCCAGACGGTGCTGTGCGTCGGCGACCTCATGCTGGACGAGTT
CGTTTACGGCGAGGTGTCGCGGATCTCGCCGGAGGCACCCGCGCCCGTCATCGCGGTCCAGCGCAGCGAAACCAATATCG
GCGGCGCCGGCAATGTCGCGCGCAACATCGCAGCGCTCGGCGCGCGCGGCATCTTCGTCGGCCTGGTCGGGCAGGACGCA
GCGGGCGACCAGCTCGAGGCCGAGCTTGCGAAGGTTGATGGCATCGAGAGTGTCCTGGTGCGCGACCCGTCGCGGCCGAC
GACGCGCAAGGTGCGCTTCGTCTCGGAGCATTTCTCGACCCACATGCTGCGTGCTGACTGGGAGCGCGCCGCGCCTGCGT
CCGAGGACATCGAGCAGAAGCTGATCGCCGCCATCCTGCCGCTGATCGCGCGGGCCGACATCGTGCTGCTGTCGGACTAC
GCCAAGGGCGTGCTGACCGCGCGCGTGCTGCGCAATGTGATCGACGCGACGCGCAGGGCCGGCAAGCGCGTGATCGTCGA
TCCCAAGAGCGCGAATCTCGCGATCTATCGCGGTGCCAGCGTGCTGACGCCGAACCGCAAGGAATTCTCGGAAGCGACCC
GCAGCCGCGCCGAGACCGAGCAGGAGATCGCCGCGGCCGCGCAGGATGCGATCTATCTGGCCGATTGCGAGGCGATCCTG
GTGACGCAGAGCGAGCGCGGCATGACCCTGGTGCCGCGCCAGGGCGATCCGATCCACGTGCCGGCCTATCCGGTGAAGGT
GCGCGACGTCTCCGGCGCCGGCGATACAGTCGTCGCCGTGCTGGCGGCAGCGCTCGCCGCCGGCGCAGCCTGGGAAGACG
CGCTGCGGATGGCGAGCGCGGCAGCGGCCGTAGCTGTCAGCAAGCAGGGGACGGCAAGCGTGACATCGGCGGAGCTGCGC
CGCAAGATTCTGCCGCATGCCTACCGTGCCGCCGAAGAAAAGATCATCGAGAACGATGGCGAACTCGCAACGCGTGTCGC
CGTTTGGCGAAGCGAAGGTCTGCGCGTCGGCTTCACCAATGGCTGCTTCGACATCCTGCATCCGGGCCATGTGAAGGTAC
TGACGGCGGCGCGCGCCGCCTGCGACCGGCTCGTCGTTGGACTGAACAGCGACACTTCGGTCAAGCGATTGAAGGGTGAG
AGCCGTCCGGTGCAGGACGAGCGCGCGCGGGCCGAAGTGCTGGCCGCATTGGAAGCGGTGGATCTTGTTGCGATCTTTAC
GGAGGAGACGCCGCTGCGCCTGATCGAGCTGGTGCGACCGAGCGTCCTGGTCAAGGGCGGCGACTACACGCGAGAGCAGG
TGGTGGGCCACGAGGTGGTCGAGGCCGCCGGCGGCGAGGTGCTGTTGATCGACATCCTGAAGGGACACAGCACGACAGCG
CTGGTCGATCGTGCCCGGAGCGATCAGCGATGA

Upstream 100 bases:

>100_bases
CTCGAGGATGCCGTGGCCGCCTATGTCCGAGACTATCTCGATTCCGCCGACCGCTTCCGCTAGACGAACTGTTCTGGATC
ACCTACCGAGACTGCCGCTG

Downstream 100 bases:

>100_bases
GCGCGCTTGCAGGTCATCCCGGCCCGGCGCTGCTGGCCCGCCGCTGGCGCGATCCGGCGGCGTGGCGGACCACGGTGGAC
GTCGTCGCCGTTCTGCTGGC

Product: bifunctional protein RfaE (involved in ADP-L-glycero-D-manno-heptose synthesis) kinase/sugar nucleotide transferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MFDFDDLSQAIARQTVLCVGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNIGGAGNVARNIAALGARGIFVGLVGQDA
AGDQLEAELAKVDGIESVLVRDPSRPTTRKVRFVSEHFSTHMLRADWERAAPASEDIEQKLIAAILPLIARADIVLLSDY
AKGVLTARVLRNVIDATRRAGKRVIVDPKSANLAIYRGASVLTPNRKEFSEATRSRAETEQEIAAAAQDAIYLADCEAIL
VTQSERGMTLVPRQGDPIHVPAYPVKVRDVSGAGDTVVAVLAAALAAGAAWEDALRMASAAAAVAVSKQGTASVTSAELR
RKILPHAYRAAEEKIIENDGELATRVAVWRSEGLRVGFTNGCFDILHPGHVKVLTAARAACDRLVVGLNSDTSVKRLKGE
SRPVQDERARAEVLAALEAVDLVAIFTEETPLRLIELVRPSVLVKGGDYTREQVVGHEVVEAAGGEVLLIDILKGHSTTA
LVDRARSDQR

Sequences:

>Translated_490_residues
MFDFDDLSQAIARQTVLCVGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNIGGAGNVARNIAALGARGIFVGLVGQDA
AGDQLEAELAKVDGIESVLVRDPSRPTTRKVRFVSEHFSTHMLRADWERAAPASEDIEQKLIAAILPLIARADIVLLSDY
AKGVLTARVLRNVIDATRRAGKRVIVDPKSANLAIYRGASVLTPNRKEFSEATRSRAETEQEIAAAAQDAIYLADCEAIL
VTQSERGMTLVPRQGDPIHVPAYPVKVRDVSGAGDTVVAVLAAALAAGAAWEDALRMASAAAAVAVSKQGTASVTSAELR
RKILPHAYRAAEEKIIENDGELATRVAVWRSEGLRVGFTNGCFDILHPGHVKVLTAARAACDRLVVGLNSDTSVKRLKGE
SRPVQDERARAEVLAALEAVDLVAIFTEETPLRLIELVRPSVLVKGGDYTREQVVGHEVVEAAGGEVLLIDILKGHSTTA
LVDRARSDQR
>Mature_490_residues
MFDFDDLSQAIARQTVLCVGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNIGGAGNVARNIAALGARGIFVGLVGQDA
AGDQLEAELAKVDGIESVLVRDPSRPTTRKVRFVSEHFSTHMLRADWERAAPASEDIEQKLIAAILPLIARADIVLLSDY
AKGVLTARVLRNVIDATRRAGKRVIVDPKSANLAIYRGASVLTPNRKEFSEATRSRAETEQEIAAAAQDAIYLADCEAIL
VTQSERGMTLVPRQGDPIHVPAYPVKVRDVSGAGDTVVAVLAAALAAGAAWEDALRMASAAAAVAVSKQGTASVTSAELR
RKILPHAYRAAEEKIIENDGELATRVAVWRSEGLRVGFTNGCFDILHPGHVKVLTAARAACDRLVVGLNSDTSVKRLKGE
SRPVQDERARAEVLAALEAVDLVAIFTEETPLRLIELVRPSVLVKGGDYTREQVVGHEVVEAAGGEVLLIDILKGHSTTA
LVDRARSDQR

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=484, Percent_Identity=44.2148760330579, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI1788017, Length=263, Percent_Identity=29.6577946768061, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 52471; Mature: 52471

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFDDLSQAIARQTVLCVGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNIGGAGNVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCCCCCCHHH
RNIAALGARGIFVGLVGQDAAGDQLEAELAKVDGIESVLVRDPSRPTTRKVRFVSEHFST
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHEECCCCCCCHHHHHHHHHHHHH
HMLRADWERAAPASEDIEQKLIAAILPLIARADIVLLSDYAKGVLTARVLRNVIDATRRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GKRVIVDPKSANLAIYRGASVLTPNRKEFSEATRSRAETEQEIAAAAQDAIYLADCEAIL
CCEEEECCCCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEE
VTQSERGMTLVPRQGDPIHVPAYPVKVRDVSGAGDTVVAVLAAALAAGAAWEDALRMASA
EEECCCCCEEECCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
AAAVAVSKQGTASVTSAELRRKILPHAYRAAEEKIIENDGELATRVAVWRSEGLRVGFTN
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECC
GCFDILHPGHVKVLTAARAACDRLVVGLNSDTSVKRLKGESRPVQDERARAEVLAALEAV
CCHHHCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
DLVAIFTEETPLRLIELVRPSVLVKGGDYTREQVVGHEVVEAAGGEVLLIDILKGHSTTA
HEEEEECCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
LVDRARSDQR
HHHHHHCCCC
>Mature Secondary Structure
MFDFDDLSQAIARQTVLCVGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNIGGAGNVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCCCCCCHHH
RNIAALGARGIFVGLVGQDAAGDQLEAELAKVDGIESVLVRDPSRPTTRKVRFVSEHFST
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHEECCCCCCCHHHHHHHHHHHHH
HMLRADWERAAPASEDIEQKLIAAILPLIARADIVLLSDYAKGVLTARVLRNVIDATRRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GKRVIVDPKSANLAIYRGASVLTPNRKEFSEATRSRAETEQEIAAAAQDAIYLADCEAIL
CCEEEECCCCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEE
VTQSERGMTLVPRQGDPIHVPAYPVKVRDVSGAGDTVVAVLAAALAAGAAWEDALRMASA
EEECCCCCEEECCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
AAAVAVSKQGTASVTSAELRRKILPHAYRAAEEKIIENDGELATRVAVWRSEGLRVGFTN
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECC
GCFDILHPGHVKVLTAARAACDRLVVGLNSDTSVKRLKGESRPVQDERARAEVLAALEAV
CCHHHCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
DLVAIFTEETPLRLIELVRPSVLVKGGDYTREQVVGHEVVEAAGGEVLLIDILKGHSTTA
HEEEEECCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
LVDRARSDQR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA