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The map label for this gene is rfbE [H]

Identifier: 146342042

GI number: 146342042

Start: 5389701

End: 5390450

Strand: Direct

Name: rfbE [H]

Synonym: BRADO5181

Alternate gene names: 146342042

Gene position: 5389701-5390450 (Clockwise)

Preceding gene: 146342041

Following gene: 146342043

Centisome position: 72.28

GC content: 64.0

Gene sequence:

>750_bases
ATGCCTCTGATCGAAGCCAAGCAACTCGGCATTGAATTTCCCGTAGCGCCGCCCAACGCCCGGTCGTTCAGGCACCTGGC
GATCAAGGCCGCGTCCCGCGTCGGCGGACACGTCTCCAGGAACGAGGGCTCGTTCCAGTTCGTGCAGGCGCTGGACGACA
TCAGCTTCACCCTGAAGCAGGGCGACCGGCTCGGGCTTGTCGGCCACAACGGCGCCGGCAAGACCACGCTGATCCGCGTG
CTGGCCGGCATCTACGAACCCACGCGCGGCTCATTGCGGGTGGTGGGCCGCAACGTGCCGATGTTCGACATCGGACTCGG
TATGGACGAGGAGGCGTCCGGATACGAGAACATCCGGACCCGCGGCCTCATCCTGGGCCTCTCTCCCGAGGAGATCGAAG
AGCGCGTACCCGAAATCGTGGAGTTTGCCGAGCTTGGCGATTATCTCGAACTGCCGATCCGGACGTATTCGTCGGGCATG
CTGCTTCGTCTCGTGTTCTCGATCGCAGCCTCCATCCACGGCGACATCATCCTGATGGACGAGTGGATCGCCGTCGGCGA
CGCGCAGTTTCGCAAGAAGACCCATGACCGGCTGCAGGACATCACCATGCGTTCCGGGATCGTGGTGCTGGCCTCTCACG
ATCACGGCCTGCTGCGCGAAACCTGCAATCTCGGGCTCTATCTCGACGGCGGACGCGTGCGCGCCTTCGGTGCGCTGGAG
GACGTTCTCAAAGAGCTGCCCGCGGCTTGA

Upstream 100 bases:

>100_bases
GCTGACGGCCGCCATGATCTACATCTACACGCGCTATCGCTCGCGCGTCGTGTACTGGGCCTGACGTGACAGCCATTCAG
GACCTCGGGACCAGCCTCTC

Downstream 100 bases:

>100_bases
GCTGTCCAATGCGACAGCATGCGGTATAGAGTTCGCTGAGCATGACCCCGACGACCGCTTCACCGACCGTTACACTGGTT
GTCCCGAACTTCAATCACGC

Product: putative O-antigen/LPS export system ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPLIEAKQLGIEFPVAPPNARSFRHLAIKAASRVGGHVSRNEGSFQFVQALDDISFTLKQGDRLGLVGHNGAGKTTLIRV
LAGIYEPTRGSLRVVGRNVPMFDIGLGMDEEASGYENIRTRGLILGLSPEEIEERVPEIVEFAELGDYLELPIRTYSSGM
LLRLVFSIAASIHGDIILMDEWIAVGDAQFRKKTHDRLQDITMRSGIVVLASHDHGLLRETCNLGLYLDGGRVRAFGALE
DVLKELPAA

Sequences:

>Translated_249_residues
MPLIEAKQLGIEFPVAPPNARSFRHLAIKAASRVGGHVSRNEGSFQFVQALDDISFTLKQGDRLGLVGHNGAGKTTLIRV
LAGIYEPTRGSLRVVGRNVPMFDIGLGMDEEASGYENIRTRGLILGLSPEEIEERVPEIVEFAELGDYLELPIRTYSSGM
LLRLVFSIAASIHGDIILMDEWIAVGDAQFRKKTHDRLQDITMRSGIVVLASHDHGLLRETCNLGLYLDGGRVRAFGALE
DVLKELPAA
>Mature_248_residues
PLIEAKQLGIEFPVAPPNARSFRHLAIKAASRVGGHVSRNEGSFQFVQALDDISFTLKQGDRLGLVGHNGAGKTTLIRVL
AGIYEPTRGSLRVVGRNVPMFDIGLGMDEEASGYENIRTRGLILGLSPEEIEERVPEIVEFAELGDYLELPIRTYSSGML
LRLVFSIAASIHGDIILMDEWIAVGDAQFRKKTHDRLQDITMRSGIVVLASHDHGLLRETCNLGLYLDGGRVRAFGALED
VLKELPAA

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=206, Percent_Identity=25.7281553398058, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI27436953, Length=203, Percent_Identity=23.6453201970443, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27477115, Length=185, Percent_Identity=28.6486486486486, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI6005701, Length=200, Percent_Identity=26.5, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI105990541, Length=205, Percent_Identity=27.3170731707317, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI87081709, Length=210, Percent_Identity=25.2380952380952, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48995001, Length=198, Percent_Identity=26.7676767676768, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787547, Length=215, Percent_Identity=26.5116279069767, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789751, Length=203, Percent_Identity=32.512315270936, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081791, Length=183, Percent_Identity=25.6830601092896, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788761, Length=218, Percent_Identity=26.1467889908257, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1786398, Length=202, Percent_Identity=23.7623762376238, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994943, Length=258, Percent_Identity=22.093023255814, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=207, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=209, Percent_Identity=28.7081339712919, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24666092, Length=216, Percent_Identity=25, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI116007184, Length=186, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221500365, Length=186, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.30 [C]

Molecular weight: Translated: 27287; Mature: 27156

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIEAKQLGIEFPVAPPNARSFRHLAIKAASRVGGHVSRNEGSFQFVQALDDISFTLKQ
CCCCCCHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECC
GDRLGLVGHNGAGKTTLIRVLAGIYEPTRGSLRVVGRNVPMFDIGLGMDEEASGYENIRT
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHH
RGLILGLSPEEIEERVPEIVEFAELGDYLELPIRTYSSGMLLRLVFSIAASIHGDIILMD
CEEEEECCHHHHHHHHHHHHHHHHHCCCEECCHHHCCCCHHHHHHHHHHHHHCCCEEEEE
EWIAVGDAQFRKKTHDRLQDITMRSGIVVLASHDHGLLRETCNLGLYLDGGRVRAFGALE
CEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHCCCCEEEECCCEEEHHHHH
DVLKELPAA
HHHHHCCCC
>Mature Secondary Structure 
PLIEAKQLGIEFPVAPPNARSFRHLAIKAASRVGGHVSRNEGSFQFVQALDDISFTLKQ
CCCCCHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECC
GDRLGLVGHNGAGKTTLIRVLAGIYEPTRGSLRVVGRNVPMFDIGLGMDEEASGYENIRT
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHH
RGLILGLSPEEIEERVPEIVEFAELGDYLELPIRTYSSGMLLRLVFSIAASIHGDIILMD
CEEEEECCHHHHHHHHHHHHHHHHHCCCEECCHHHCCCCHHHHHHHHHHHHHCCCEEEEE
EWIAVGDAQFRKKTHDRLQDITMRSGIVVLASHDHGLLRETCNLGLYLDGGRVRAFGALE
CEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHCCCCEEEECCCEEEHHHHH
DVLKELPAA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]