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The map label for this gene is rfbB [H]

Identifier: 146342030

GI number: 146342030

Start: 5378369

End: 5379367

Strand: Direct

Name: rfbB [H]

Synonym: BRADO5168

Alternate gene names: 146342030

Gene position: 5378369-5379367 (Clockwise)

Preceding gene: 146342029

Following gene: 146342031

Centisome position: 72.13

GC content: 65.07

Gene sequence:

>999_bases
ATGGCTGCGAACGAAAAGTACCTGATCCTCGGCTCGAACTCCTTCTCGGGCGCAACCTTCGTCGACTTCCTGGCCGCGGC
CGGTCACGACGTGATCGCGACCAGCCGCTCGGACGAGCCGCATGACGCGTTCCTGCCCTACAAGTGGCAGAAGCGCCCCG
GCAAGGTCCGCTTTAAACGCGTCGACCTCAACCACGATCTGGATGTGCTGAAGGCGCTGCTGGCAAGCGAGCGGCCGACG
CATGTCGTGAACTTCGCGGCGCAGAGCATGGTCGGCGAGAGCTGGCTGTATCCGGACCATTGGATGATGACCAACGTCGT
CTCCGCCGTCCGCCTGCACGACATCCTGCGCAACTATGACGGCCTCGACCGCTACGTCCACGTCACGACCCCGGAAGTCT
ACGGCTCGACCGAGGGCTGGGTGCGCGAGGATGCGCCGTTCAACCCGTCGACGCCCTACGCGGTGTCGCGTGCGGCCGGC
GACATGAGCCTGCGCACCTATTTCGCCAACTACCAATTCCCTGTCGTCTTCACCCGCGCCGCCAATGTCTACGGCCCGGG
CCAGCAGCTCTACCGCATCGTGCCGCGCACCATCGTGGCGGCGATGACCGGGCAGAAGCTGCGCCTCGACGGCGGCGGCA
AGTCGGTGCGCGTGTTCATCCACATGACCGACGTGTCGGATGCGACGGTGAAGATCGGCCGCAGCGGCAAGCCCGGCGAG
ACCTATCACATCTCCGGCTACGAGCTGGTCTCGATCCGGACGCTGGTCGAGATGATCCTCGCACGGCTCGGCAAGAACTT
CGAGGACTGCGTCGAGATCGGACCGGAGCGGCCAGGCAAGGACACCGCCTACACGCTCGACAGCTTCAAGCTGCGCACCG
AGCTCGGCTGGCGCGACACCTTCGCCCTGGAGCAGGGCATCGACGACGTCATCGCCTGGGCGCGGCGCTTCGAGGATGTC
ATGAACAAGCTGCCGACCAAGTACGAGCATAAACCCTGA

Upstream 100 bases:

>100_bases
CGAGGACGCGCAGATGGTGATCGGCCACATGATCATGCAGTGGCTGTACGCCCAGCGTGGCGACATCATCACGACGCGCG
AGGTCTGACGGACAGGGACG

Downstream 100 bases:

>100_bases
TCTATACCTGCGCCTCCCCGGGAGGCGCGACTGGAGAGCACGATGAACGTGGCAGTCCGGAACACCGCGGCCAAGCCCGA
CGTGGCCGCCCTGAAGGCGC

Product: putative dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAANEKYLILGSNSFSGATFVDFLAAAGHDVIATSRSDEPHDAFLPYKWQKRPGKVRFKRVDLNHDLDVLKALLASERPT
HVVNFAAQSMVGESWLYPDHWMMTNVVSAVRLHDILRNYDGLDRYVHVTTPEVYGSTEGWVREDAPFNPSTPYAVSRAAG
DMSLRTYFANYQFPVVFTRAANVYGPGQQLYRIVPRTIVAAMTGQKLRLDGGGKSVRVFIHMTDVSDATVKIGRSGKPGE
TYHISGYELVSIRTLVEMILARLGKNFEDCVEIGPERPGKDTAYTLDSFKLRTELGWRDTFALEQGIDDVIAWARRFEDV
MNKLPTKYEHKP

Sequences:

>Translated_332_residues
MAANEKYLILGSNSFSGATFVDFLAAAGHDVIATSRSDEPHDAFLPYKWQKRPGKVRFKRVDLNHDLDVLKALLASERPT
HVVNFAAQSMVGESWLYPDHWMMTNVVSAVRLHDILRNYDGLDRYVHVTTPEVYGSTEGWVREDAPFNPSTPYAVSRAAG
DMSLRTYFANYQFPVVFTRAANVYGPGQQLYRIVPRTIVAAMTGQKLRLDGGGKSVRVFIHMTDVSDATVKIGRSGKPGE
TYHISGYELVSIRTLVEMILARLGKNFEDCVEIGPERPGKDTAYTLDSFKLRTELGWRDTFALEQGIDDVIAWARRFEDV
MNKLPTKYEHKP
>Mature_331_residues
AANEKYLILGSNSFSGATFVDFLAAAGHDVIATSRSDEPHDAFLPYKWQKRPGKVRFKRVDLNHDLDVLKALLASERPTH
VVNFAAQSMVGESWLYPDHWMMTNVVSAVRLHDILRNYDGLDRYVHVTTPEVYGSTEGWVREDAPFNPSTPYAVSRAAGD
MSLRTYFANYQFPVVFTRAANVYGPGQQLYRIVPRTIVAAMTGQKLRLDGGGKSVRVFIHMTDVSDATVKIGRSGKPGET
YHISGYELVSIRTLVEMILARLGKNFEDCVEIGPERPGKDTAYTLDSFKLRTELGWRDTFALEQGIDDVIAWARRFEDVM
NKLPTKYEHKP

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=257, Percent_Identity=32.295719844358, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI48994969, Length=330, Percent_Identity=27.8787878787879, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788353, Length=282, Percent_Identity=29.0780141843972, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532424, Length=325, Percent_Identity=26.7692307692308, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568069, Length=272, Percent_Identity=30.8823529411765, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37415; Mature: 37284

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANEKYLILGSNSFSGATFVDFLAAAGHDVIATSRSDEPHDAFLPYKWQKRPGKVRFKR
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCCCCCEEEEE
VDLNHDLDVLKALLASERPTHVVNFAAQSMVGESWLYPDHWMMTNVVSAVRLHDILRNYD
ECCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLDRYVHVTTPEVYGSTEGWVREDAPFNPSTPYAVSRAAGDMSLRTYFANYQFPVVFTRA
CCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCEEHHCCCCCEEEEEECCCCCEEEEEEC
ANVYGPGQQLYRIVPRTIVAAMTGQKLRLDGGGKSVRVFIHMTDVSDATVKIGRSGKPGE
CCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCC
TYHISGYELVSIRTLVEMILARLGKNFEDCVEIGPERPGKDTAYTLDSFKLRTELGWRDT
EEEECCEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCEEEEECCCCCHH
FALEQGIDDVIAWARRFEDVMNKLPTKYEHKP
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AANEKYLILGSNSFSGATFVDFLAAAGHDVIATSRSDEPHDAFLPYKWQKRPGKVRFKR
CCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCCCCCEEEEE
VDLNHDLDVLKALLASERPTHVVNFAAQSMVGESWLYPDHWMMTNVVSAVRLHDILRNYD
ECCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLDRYVHVTTPEVYGSTEGWVREDAPFNPSTPYAVSRAAGDMSLRTYFANYQFPVVFTRA
CCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCEEHHCCCCCEEEEEECCCCCEEEEEEC
ANVYGPGQQLYRIVPRTIVAAMTGQKLRLDGGGKSVRVFIHMTDVSDATVKIGRSGKPGE
CCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCC
TYHISGYELVSIRTLVEMILARLGKNFEDCVEIGPERPGKDTAYTLDSFKLRTELGWRDT
EEEECCEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCEEEEECCCCCHH
FALEQGIDDVIAWARRFEDVMNKLPTKYEHKP
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]