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The map label for this gene is ytcB [H]

Identifier: 146342028

GI number: 146342028

Start: 5376813

End: 5377751

Strand: Direct

Name: ytcB [H]

Synonym: BRADO5166

Alternate gene names: 146342028

Gene position: 5376813-5377751 (Clockwise)

Preceding gene: 146342027

Following gene: 146342029

Centisome position: 72.11

GC content: 60.38

Gene sequence:

>939_bases
ATGAAGATATTCGTGACCGGAGCGTGCGGCTACAAGGGCAGCGTGCTCGTGCCGAAGCTGTTGAAGGCCGGCCACAAGGT
CGTCGCGTTCGACATCATGTGGTTCGGCAACTTCCTCGAGCCGCATCCGGATCTCACCGTCGTCCAGGGCGACGTGCGCA
ATCCCGACGCGATCGACCTGACCGGCGTGGATGCCATCATCCATCTCTCCAGCGTCGCCAATGATCCGTGCGGCGATCTC
GATCCGAAGCTGACCTGGGAGATTTCCTGTCTCGCCACGATGCAGCTCGCCGACCGCGCCTATCGCCACGGCATCAAGCG
CTTCATCTACGCCTCCTCCGGCAGCGTCTATGGCGTGAAGGAAGAGGAGCAGGTCACCGAGGATCTCGAGCTGCTGCCGA
TCTCGGAGTACAACAAGACCAAGATGTGCGGCGAGCGCATCGTGCTGTCCTACAAGGACGACATGGTGGTGCAGATCGTA
CGCCCGGCCACCGTGTGCGGCTACTCTCCGCGCCAGCGCCTCGATGTGTCGGTGAACATGCTGACGATGCAGGCGCTGAT
GAACGGCCGCATCACCGTGTTCGGCGGCGACCAGACCCGGCCGAACATTCATATCGACGACATCACCGATCTCTATCTCT
TCCTGCTCGATCATCCCGAGCACACGGGCGTCTACAATGCGGGCTTCGAGAACATCTCGATCCTCGACATTGCCAATATG
GTCACCAAGCACGTGCCGGCCGAGGTTATCGTGACGCCCTCGAATGATCCGCGCAGCTATCGCGTCAACTCCGACCGCCT
GCTCGCGACCGGCTTCCGTCCGAAGAAGACGGTGGAGGACGCGATCAAGGAAATCATCGGCATGTACTCGCAAGGCGCCC
TCAAGAACGAGGACCGCTGGTACAATCTGAAATGGATGGAGAAGGAGGTCGTGAAATGA

Upstream 100 bases:

>100_bases
CTTGACGGGCGGCTGTGGCTGCCTCCCCCACCAATTTGCATAAAGGTGCGCGCGACGCGCCAGCCGGCGAGACGACAGGC
GAGCCACGGGGATTGCGCAG

Downstream 100 bases:

>100_bases
ACGTTCTCGCCTTCAAGACTCCCGGCAGCCTGTTCGCCGACTACACCAGCCGCTTCAGCACCGTGCTGAAGGATTTCGAC
TGGGCCCCCGTCGAGAAGCT

Product: putative sugar-nucleotide epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKIFVTGACGYKGSVLVPKLLKAGHKVVAFDIMWFGNFLEPHPDLTVVQGDVRNPDAIDLTGVDAIIHLSSVANDPCGDL
DPKLTWEISCLATMQLADRAYRHGIKRFIYASSGSVYGVKEEEQVTEDLELLPISEYNKTKMCGERIVLSYKDDMVVQIV
RPATVCGYSPRQRLDVSVNMLTMQALMNGRITVFGGDQTRPNIHIDDITDLYLFLLDHPEHTGVYNAGFENISILDIANM
VTKHVPAEVIVTPSNDPRSYRVNSDRLLATGFRPKKTVEDAIKEIIGMYSQGALKNEDRWYNLKWMEKEVVK

Sequences:

>Translated_312_residues
MKIFVTGACGYKGSVLVPKLLKAGHKVVAFDIMWFGNFLEPHPDLTVVQGDVRNPDAIDLTGVDAIIHLSSVANDPCGDL
DPKLTWEISCLATMQLADRAYRHGIKRFIYASSGSVYGVKEEEQVTEDLELLPISEYNKTKMCGERIVLSYKDDMVVQIV
RPATVCGYSPRQRLDVSVNMLTMQALMNGRITVFGGDQTRPNIHIDDITDLYLFLLDHPEHTGVYNAGFENISILDIANM
VTKHVPAEVIVTPSNDPRSYRVNSDRLLATGFRPKKTVEDAIKEIIGMYSQGALKNEDRWYNLKWMEKEVVK
>Mature_312_residues
MKIFVTGACGYKGSVLVPKLLKAGHKVVAFDIMWFGNFLEPHPDLTVVQGDVRNPDAIDLTGVDAIIHLSSVANDPCGDL
DPKLTWEISCLATMQLADRAYRHGIKRFIYASSGSVYGVKEEEQVTEDLELLPISEYNKTKMCGERIVLSYKDDMVVQIV
RPATVCGYSPRQRLDVSVNMLTMQALMNGRITVFGGDQTRPNIHIDDITDLYLFLLDHPEHTGVYNAGFENISILDIANM
VTKHVPAEVIVTPSNDPRSYRVNSDRLLATGFRPKKTVEDAIKEIIGMYSQGALKNEDRWYNLKWMEKEVVK

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=308, Percent_Identity=24.6753246753247, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=22.5225225225225, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786974, Length=281, Percent_Identity=24.9110320284698, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=278, Percent_Identity=26.978417266187, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35025; Mature: 35025

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFVTGACGYKGSVLVPKLLKAGHKVVAFDIMWFGNFLEPHPDLTVVQGDVRNPDAIDL
CEEEEEECCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEE
TGVDAIIHLSSVANDPCGDLDPKLTWEISCLATMQLADRAYRHGIKRFIYASSGSVYGVK
ECHHHHEEHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHEEEEECCCCEEECC
EEEQVTEDLELLPISEYNKTKMCGERIVLSYKDDMVVQIVRPATVCGYSPRQRLDVSVNM
CHHHHHCCCEEEECCCCCCHHHCCCEEEEEECCCEEEEEECCHHHCCCCCCCEEEEEHHH
LTMQALMNGRITVFGGDQTRPNIHIDDITDLYLFLLDHPEHTGVYNAGFENISILDIANM
HHHHHHHCCCEEEEECCCCCCCEEECCHHEEEEEEECCCCCCCEEECCCCCEEEHHHHHH
VTKHVPAEVIVTPSNDPRSYRVNSDRLLATGFRPKKTVEDAIKEIIGMYSQGALKNEDRW
HHHCCCCEEEEECCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCE
YNLKWMEKEVVK
EEEEEHHHHHCC
>Mature Secondary Structure
MKIFVTGACGYKGSVLVPKLLKAGHKVVAFDIMWFGNFLEPHPDLTVVQGDVRNPDAIDL
CEEEEEECCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEE
TGVDAIIHLSSVANDPCGDLDPKLTWEISCLATMQLADRAYRHGIKRFIYASSGSVYGVK
ECHHHHEEHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHEEEEECCCCEEECC
EEEQVTEDLELLPISEYNKTKMCGERIVLSYKDDMVVQIVRPATVCGYSPRQRLDVSVNM
CHHHHHCCCEEEECCCCCCHHHCCCEEEEEECCCEEEEEECCHHHCCCCCCCEEEEEHHH
LTMQALMNGRITVFGGDQTRPNIHIDDITDLYLFLLDHPEHTGVYNAGFENISILDIANM
HHHHHHHCCCEEEEECCCCCCCEEECCHHEEEEEEECCCCCCCEEECCCCCEEEHHHHHH
VTKHVPAEVIVTPSNDPRSYRVNSDRLLATGFRPKKTVEDAIKEIIGMYSQGALKNEDRW
HHHCCCCEEEEECCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCE
YNLKWMEKEVVK
EEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]