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The map label for this gene is hcaT [H]

Identifier: 146341952

GI number: 146341952

Start: 5285346

End: 5286533

Strand: Reverse

Name: hcaT [H]

Synonym: BRADO5088

Alternate gene names: 146341952

Gene position: 5286533-5285346 (Counterclockwise)

Preceding gene: 146341955

Following gene: 146341950

Centisome position: 70.9

GC content: 67.59

Gene sequence:

>1188_bases
ATGCCACTGTCCCGACAAATCTCCACCACATCGCCGGTATTGTCGCGTCGATTCGCGGGGAGGTTGGGGCTGTTCTATGG
CGCCGTCTTCGCGCTGTCGGGCACGCATCTCCCGTTTTTTCCCGTGTGGCTACGGGCGATCGGGCTTGATGCCTCATGGA
TCGGCATCATCATCGCGGTTCCAGCCGTCACCCGTTTCACCGTCCTGCCGCTGATCACCGCGCTTGCCGAGCGCCGGCAC
GCTTTGCGCGGCGCGCTGCGGCTGACGACGTTCGCAACTGCGTCAGGCTTTGCTGTTCTCGCCCTCCAACATCAGCCCTG
GGTGGTCTTTCTCGTCTATGTCGGGCTTTGTAGTCTGTGGACGCCGGTCGTGCCGCTGACGGATGCCTACGCGCTGCGCG
GCGTGATGAGCTATGGCCTGAACTACGGCCGCCTGCGGCTCTGGGGATCGGCCGCCTTCATCGTCGGGACCTTGCTGTGC
GGCGGCCTCGCCGACATCGTTCCGGCGCCGCAGCTGGTCTGGATCATCGTCGCGCTGGCGCTCTGTGGCGCCGCGAGCGG
ACTACTGCTGCAGCCGCTGCCGCCGTTGCCGCGCAGCACGGGCCCGGCGCAGGATGGCCGCAGCCTGCTCGGCAGTCCGC
GTTTCCTGTGCATCATCGCAGCGTCGGCGCTGATCCAGGGCAGCCACGCCGCCTACTACACTTTTTCCGCCATCGAATGG
AAGGCGCAGGGTCTGACCGGACTTACCATTGCATGGCTGTGGACCATGGGCGTGCTCGCCGAGATCGTGGTGTTCGCGCT
GTCACCCCGGTTCACGCTCTCGCCGGCCATCCTCGTTGTCATCGGCGCCATCGCCGCGGTGCTGCGCTGGACGCTGACGG
CCCAGGAGCCGCACGCGGCAGGCCTCGCGGCGGTCCAGATCAGTCATGGCTTCACCTTCGGGCTCACGCTTGTCGGCACG
ATGGGGCTGCTGGTCCGCGAGGTGCCGGCGCATCTGACCGCGCGCGGGCAGGGCTATTACTCCGCTGCAAGCGGCGTCGT
CGGCTCGGCGGCCTCGATCCTGTCGGGACCGCTCTACGCGGCCTATGGACCGGGCGTCTACTACGTCATGGCCGTGATGG
CCGGTCTCGGCGGCGCGCTGATGTGGCTTGCGCGTGCCGGGCTGTCGCCTCAGCCCCACAGGGCCTGA

Upstream 100 bases:

>100_bases
GACTTCATCGACCATGACAGACCACGACAGACCCATCGCGCAAATTGCTGCGCGTTGGCATAACGATCACCATGATATTG
ACCGAATCGCAATTGAATTG

Downstream 100 bases:

>100_bases
TCGGGCGGGTAGACCAGGCTGTCCTCGTAGTAGAGCCCGTGCGGGCAGTCCTCGGCGAGCAGCAGCGGCCCGTCGAGGTC
GACATAGCGGGCGAGCGGCG

Product: major facilitator superfamily 3-phenylpropionic acid transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPLSRQISTTSPVLSRRFAGRLGLFYGAVFALSGTHLPFFPVWLRAIGLDASWIGIIIAVPAVTRFTVLPLITALAERRH
ALRGALRLTTFATASGFAVLALQHQPWVVFLVYVGLCSLWTPVVPLTDAYALRGVMSYGLNYGRLRLWGSAAFIVGTLLC
GGLADIVPAPQLVWIIVALALCGAASGLLLQPLPPLPRSTGPAQDGRSLLGSPRFLCIIAASALIQGSHAAYYTFSAIEW
KAQGLTGLTIAWLWTMGVLAEIVVFALSPRFTLSPAILVVIGAIAAVLRWTLTAQEPHAAGLAAVQISHGFTFGLTLVGT
MGLLVREVPAHLTARGQGYYSAASGVVGSAASILSGPLYAAYGPGVYYVMAVMAGLGGALMWLARAGLSPQPHRA

Sequences:

>Translated_395_residues
MPLSRQISTTSPVLSRRFAGRLGLFYGAVFALSGTHLPFFPVWLRAIGLDASWIGIIIAVPAVTRFTVLPLITALAERRH
ALRGALRLTTFATASGFAVLALQHQPWVVFLVYVGLCSLWTPVVPLTDAYALRGVMSYGLNYGRLRLWGSAAFIVGTLLC
GGLADIVPAPQLVWIIVALALCGAASGLLLQPLPPLPRSTGPAQDGRSLLGSPRFLCIIAASALIQGSHAAYYTFSAIEW
KAQGLTGLTIAWLWTMGVLAEIVVFALSPRFTLSPAILVVIGAIAAVLRWTLTAQEPHAAGLAAVQISHGFTFGLTLVGT
MGLLVREVPAHLTARGQGYYSAASGVVGSAASILSGPLYAAYGPGVYYVMAVMAGLGGALMWLARAGLSPQPHRA
>Mature_394_residues
PLSRQISTTSPVLSRRFAGRLGLFYGAVFALSGTHLPFFPVWLRAIGLDASWIGIIIAVPAVTRFTVLPLITALAERRHA
LRGALRLTTFATASGFAVLALQHQPWVVFLVYVGLCSLWTPVVPLTDAYALRGVMSYGLNYGRLRLWGSAAFIVGTLLCG
GLADIVPAPQLVWIIVALALCGAASGLLLQPLPPLPRSTGPAQDGRSLLGSPRFLCIIAASALIQGSHAAYYTFSAIEWK
AQGLTGLTIAWLWTMGVLAEIVVFALSPRFTLSPAILVVIGAIAAVLRWTLTAQEPHAAGLAAVQISHGFTFGLTLVGTM
GLLVREVPAHLTARGQGYYSAASGVVGSAASILSGPLYAAYGPGVYYVMAVMAGLGGALMWLARAGLSPQPHRA

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=366, Percent_Identity=33.6065573770492, Blast_Score=148, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 41552; Mature: 41420

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRQISTTSPVLSRRFAGRLGLFYGAVFALSGTHLPFFPVWLRAIGLDASWIGIIIAV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
PAVTRFTVLPLITALAERRHALRGALRLTTFATASGFAVLALQHQPWVVFLVYVGLCSLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEECCCHHHHHHHHHHHHHH
TPVVPLTDAYALRGVMSYGLNYGRLRLWGSAAFIVGTLLCGGLADIVPAPQLVWIIVALA
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
LCGAASGLLLQPLPPLPRSTGPAQDGRSLLGSPRFLCIIAASALIQGSHAAYYTFSAIEW
HHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEHHHHHHHHHCCCCCEEEEEEEEEE
KAQGLTGLTIAWLWTMGVLAEIVVFALSPRFTLSPAILVVIGAIAAVLRWTLTAQEPHAA
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GLAAVQISHGFTFGLTLVGTMGLLVREVPAHLTARGQGYYSAASGVVGSAASILSGPLYA
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCHH
AYGPGVYYVMAVMAGLGGALMWLARAGLSPQPHRA
HCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLSRQISTTSPVLSRRFAGRLGLFYGAVFALSGTHLPFFPVWLRAIGLDASWIGIIIAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
PAVTRFTVLPLITALAERRHALRGALRLTTFATASGFAVLALQHQPWVVFLVYVGLCSLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEECCCHHHHHHHHHHHHHH
TPVVPLTDAYALRGVMSYGLNYGRLRLWGSAAFIVGTLLCGGLADIVPAPQLVWIIVALA
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
LCGAASGLLLQPLPPLPRSTGPAQDGRSLLGSPRFLCIIAASALIQGSHAAYYTFSAIEW
HHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEHHHHHHHHHCCCCCEEEEEEEEEE
KAQGLTGLTIAWLWTMGVLAEIVVFALSPRFTLSPAILVVIGAIAAVLRWTLTAQEPHAA
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GLAAVQISHGFTFGLTLVGTMGLLVREVPAHLTARGQGYYSAASGVVGSAASILSGPLYA
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCHH
AYGPGVYYVMAVMAGLGGALMWLARAGLSPQPHRA
HCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]