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The map label for this gene is mhpC [H]

Identifier: 146341249

GI number: 146341249

Start: 4518881

End: 4519885

Strand: Direct

Name: mhpC [H]

Synonym: BRADO4325

Alternate gene names: 146341249

Gene position: 4518881-4519885 (Clockwise)

Preceding gene: 146341248

Following gene: 146341256

Centisome position: 60.6

GC content: 63.68

Gene sequence:

>1005_bases
ATGTCCCGTTTCGCTGTTGCGCTCTGCGCCGCCCTGATCGCTGTCGTCCCCTCCATCGCCTCGGCCGCGGAGGCGGCGCG
CGAGCCCTATGGCATCAATCTCGAGGGCTTTGCCTATCCTTATCCGGTCGGCCTGCTGCCGCTGGTCAATGACGGCGAGC
AGCTTCGGATGGCCTACATGGATGTCGCGCCGGCGCAGCCCAACGGCCGCACCGCGCTGTTGCTGCACGGCCGGAATTTT
CCCGCGAGCTACTGGGCGCCGGTCATCAGGACGCTGACGGAGGCCGGCTTCCGCGTCGTCGTGCCCGACCAGGTCGGTTT
TGGCAAATCATCCAAGCCCGCGGGTGAGCTGCATTTCGACACCCTGGCGCGCAACACCATCGCGCTGCTCGATCACCTCG
GCATCGCCAGGGTCGAGATCGTCGCGCATTCGATGGGCGGAATGCTCGCGGTGCGAATTGGCCGCGCCTATCCCGATCGT
GTCGCGCATCTCGTGCTGACCGCGCCGATCGGGCTCGAGGATTACCGGATGTACGTGCCGCCCATCCCGACCGAAAAGAT
CATCGAGACCGAGGATAGTCTCACCGCCGAGGGCTATCGCAAGCAATTGGAGACCAACTACGCGCTGAAGCTGCCGCCCG
AGCAGGTGACGCCATTCATCGACGCGCGCTTCAACCTCAAGGGCAGTGCCGACTATCCGCGCTGGCTGAAGAGCTTTGTC
GGCTCGTATCAGATGATCTATCGCGAGCCCGTTGTGCACGAGATTCCGCTGCTGACACAGCCGACTTTGTTCATCATGGG
AGCCGACGACCACAACGCTCCGGGACGGCCGAACGCGCCTGAAGTGTTGCGCGCCAAGATGGGGCAGAACGCCGAACTCG
CGAAAGCACTCAGCAGCCAGATGCGCGATGCGCGCACGGAGGTGATTCCCAACGCCGGCCACCTCGTGTTTCTGGATGCG
CCCGAGGCCTACGACGCGCTGCTACTTGAGTTTTTAAGCAAGTAG

Upstream 100 bases:

>100_bases
TGTTTATCGCGGCAGCGCGATACAGGCTTGCCTCGGCAGCGAACGCGGGTCACTGCGCGAGGGTCGACGATGCCGTCGCC
CTCTGCTTCCCGGAGCCTCA

Downstream 100 bases:

>100_bases
CTGTTGGGAACAAACGGCAGGGACGCGGCGGGGCCGCATCCCTGTCCGGCTTCGTGAACTCACCAGTTTCGGAAGCAGCG
CTTGCCGTACGGGCCGGTGT

Product: putative alpha/beta hydrolase prolyl aminopeptidase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSRFAVALCAALIAVVPSIASAAEAAREPYGINLEGFAYPYPVGLLPLVNDGEQLRMAYMDVAPAQPNGRTALLLHGRNF
PASYWAPVIRTLTEAGFRVVVPDQVGFGKSSKPAGELHFDTLARNTIALLDHLGIARVEIVAHSMGGMLAVRIGRAYPDR
VAHLVLTAPIGLEDYRMYVPPIPTEKIIETEDSLTAEGYRKQLETNYALKLPPEQVTPFIDARFNLKGSADYPRWLKSFV
GSYQMIYREPVVHEIPLLTQPTLFIMGADDHNAPGRPNAPEVLRAKMGQNAELAKALSSQMRDARTEVIPNAGHLVFLDA
PEAYDALLLEFLSK

Sequences:

>Translated_334_residues
MSRFAVALCAALIAVVPSIASAAEAAREPYGINLEGFAYPYPVGLLPLVNDGEQLRMAYMDVAPAQPNGRTALLLHGRNF
PASYWAPVIRTLTEAGFRVVVPDQVGFGKSSKPAGELHFDTLARNTIALLDHLGIARVEIVAHSMGGMLAVRIGRAYPDR
VAHLVLTAPIGLEDYRMYVPPIPTEKIIETEDSLTAEGYRKQLETNYALKLPPEQVTPFIDARFNLKGSADYPRWLKSFV
GSYQMIYREPVVHEIPLLTQPTLFIMGADDHNAPGRPNAPEVLRAKMGQNAELAKALSSQMRDARTEVIPNAGHLVFLDA
PEAYDALLLEFLSK
>Mature_333_residues
SRFAVALCAALIAVVPSIASAAEAAREPYGINLEGFAYPYPVGLLPLVNDGEQLRMAYMDVAPAQPNGRTALLLHGRNFP
ASYWAPVIRTLTEAGFRVVVPDQVGFGKSSKPAGELHFDTLARNTIALLDHLGIARVEIVAHSMGGMLAVRIGRAYPDRV
AHLVLTAPIGLEDYRMYVPPIPTEKIIETEDSLTAEGYRKQLETNYALKLPPEQVTPFIDARFNLKGSADYPRWLKSFVG
SYQMIYREPVVHEIPLLTQPTLFIMGADDHNAPGRPNAPEVLRAKMGQNAELAKALSSQMRDARTEVIPNAGHLVFLDAP
EAYDALLLEFLSK

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=299, Percent_Identity=25.752508361204, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 36630; Mature: 36499

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFAVALCAALIAVVPSIASAAEAAREPYGINLEGFAYPYPVGLLPLVNDGEQLRMAYM
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHEEHHH
DVAPAQPNGRTALLLHGRNFPASYWAPVIRTLTEAGFRVVVPDQVGFGKSSKPAGELHFD
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHH
TLARNTIALLDHLGIARVEIVAHSMGGMLAVRIGRAYPDRVAHLVLTAPIGLEDYRMYVP
HHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCHHHHEEEEEECCCCCCCCEEECC
PIPTEKIIETEDSLTAEGYRKQLETNYALKLPPEQVTPFIDARFNLKGSADYPRWLKSFV
CCCHHHHHCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHCEEEECCCCCCHHHHHHHHH
GSYQMIYREPVVHEIPLLTQPTLFIMGADDHNAPGRPNAPEVLRAKMGQNAELAKALSSQ
HHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHH
MRDARTEVIPNAGHLVFLDAPEAYDALLLEFLSK
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SRFAVALCAALIAVVPSIASAAEAAREPYGINLEGFAYPYPVGLLPLVNDGEQLRMAYM
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHEEHHH
DVAPAQPNGRTALLLHGRNFPASYWAPVIRTLTEAGFRVVVPDQVGFGKSSKPAGELHFD
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHH
TLARNTIALLDHLGIARVEIVAHSMGGMLAVRIGRAYPDRVAHLVLTAPIGLEDYRMYVP
HHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCHHHHEEEEEECCCCCCCCEEECC
PIPTEKIIETEDSLTAEGYRKQLETNYALKLPPEQVTPFIDARFNLKGSADYPRWLKSFV
CCCHHHHHCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHCEEEECCCCCCHHHHHHHHH
GSYQMIYREPVVHEIPLLTQPTLFIMGADDHNAPGRPNAPEVLRAKMGQNAELAKALSSQ
HHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHH
MRDARTEVIPNAGHLVFLDAPEAYDALLLEFLSK
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA