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The map label for this gene is dctA

Identifier: 146340470

GI number: 146340470

Start: 3693807

End: 3695144

Strand: Direct

Name: dctA

Synonym: BRADO3510

Alternate gene names: 146340470

Gene position: 3693807-3695144 (Clockwise)

Preceding gene: 146340469

Following gene: 146340472

Centisome position: 49.54

GC content: 63.38

Gene sequence:

>1338_bases
ATGGCGGCAGCAACAGACATCGGAACGCGAGACGTTCCGTCGCAGACGGGCAAAAAGCCCTGGTACAGCATTCTCTATGT
GCAGGTCCTGATCGCGATCCTGATCGGTATCGTGGTCGGCTGGCTGTTCCCGAACCTGGCCACCAACGACTGGATCAAGG
CGCTCGGCGACGGCTTCATCAAGCTGATCAAGATGGTGATCGCGCCGATCATCTTCTGCACCGTGGTGTCGGGCATCGCG
CACATCCAGAATGCCAGCAAGGTCGGCCGGGTCGGCGTCAAGGCCCTGGTCTATTTCGAGATCGTCTCGACCTTCGCGCT
GCTGCTCGGCCTCATCGTCGGCAACCTCGCTCCGATCGGTCACGGGCTCGCCGCCAAGCCGGATGCAGCGGCGGTTGCCA
ACTACGTCAAGCAGGCCGAGGCGCAGAAGTCGGTCGACTTCGTCCTCAACATCATCCCGGACAGCGTGGTCGGCGCGCTG
GCGCGCGGCGACATCCTGCAGGTGCTGCTGTTCGCGATCCTGTTCGGCTTCGCTTTGATGGCGCTCGGCGAGCGCGGTCA
CCGGCTGCGTGACATCATCGATGACACGGCGCATGCGGTGTTCGGCGTCATCGCCATCGTCATGAAGGCCGCACCAGTCG
GAGCCTTCGGCGCCATGGCCTTTACGATCGGCAAGTACGGTCCGGCAGCGCTCGGCAACCTGATCGGCCTGATCGCGCTG
TTCTACATCACCGCCGGCCTGTTCGTCGTGATCGTGCTCGGACTGATCGCACGCTTCATCGGCTTCAGCATCTTCAAGTT
CGTCGCTTACATCAAGGACGAGCTCTTGATCGTGCTCGGCACCAGCTCCTCCGAGAGCGCGCTGCCGCAGCTGATGGAGA
AGCTGGAGCGGCTCGGCTGCTCCAAGCCGGTGGTCGGCCTGGTCGTGCCGACCGGCTATTCGTTCAATCTGGACGGCACC
AACATCTACATGACCTTGGCGACGCTGTTCATCTCGCAGGCGCTCGGCGTCGATCTCTCCTTCAGCCAGCAGGTCACCAT
CCTGATCGTCGCCATGCTGACCTCGAAAGGCGCCAGCGGCGTCACCGGCGCCGGCTTCATTACGCTGGCCGCGACCCTGT
CGGTGGTGAACCCGGCTCTGGTGCCGGGCATGGCGATCGTGTTCTCGATCGACAAGTTCATGAGCGAGGTGCGCGCGCTC
ACCAACATCACCGGCAACGGCATCGCCGCCGTGTTCGTGTCGTGGTGGGAGGGCGAGCTCGATCACGACCTGCTGCAACG
TCGGCTCAACCAGCAGATCGATCCGTCCGATGTCGAGACCGCCGTGACGACCGGCTGA

Upstream 100 bases:

>100_bases
ATCCTTCGTCGTCATTGACTCGGCCGCGACAAACTGTCTGAGGTGCGCCGCGCACGGGCTGGGACAAGCGACGCCCGATA
GACGAAGGTGGATGAGGCCG

Downstream 100 bases:

>100_bases
GGGCTCCCTGCGCCGCAGAACTCGCCAACGAAAAACGCCCAGCCGCTTCGGCCGGGCGTTTTGCATTGAAGCGCTCGAAC
GCTGTGACGGCCTACTGCCC

Product: C4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAAATDIGTRDVPSQTGKKPWYSILYVQVLIAILIGIVVGWLFPNLATNDWIKALGDGFIKLIKMVIAPIIFCTVVSGIA
HIQNASKVGRVGVKALVYFEIVSTFALLLGLIVGNLAPIGHGLAAKPDAAAVANYVKQAEAQKSVDFVLNIIPDSVVGAL
ARGDILQVLLFAILFGFALMALGERGHRLRDIIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLIAL
FYITAGLFVVIVLGLIARFIGFSIFKFVAYIKDELLIVLGTSSSESALPQLMEKLERLGCSKPVVGLVVPTGYSFNLDGT
NIYMTLATLFISQALGVDLSFSQQVTILIVAMLTSKGASGVTGAGFITLAATLSVVNPALVPGMAIVFSIDKFMSEVRAL
TNITGNGIAAVFVSWWEGELDHDLLQRRLNQQIDPSDVETAVTTG

Sequences:

>Translated_445_residues
MAAATDIGTRDVPSQTGKKPWYSILYVQVLIAILIGIVVGWLFPNLATNDWIKALGDGFIKLIKMVIAPIIFCTVVSGIA
HIQNASKVGRVGVKALVYFEIVSTFALLLGLIVGNLAPIGHGLAAKPDAAAVANYVKQAEAQKSVDFVLNIIPDSVVGAL
ARGDILQVLLFAILFGFALMALGERGHRLRDIIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLIAL
FYITAGLFVVIVLGLIARFIGFSIFKFVAYIKDELLIVLGTSSSESALPQLMEKLERLGCSKPVVGLVVPTGYSFNLDGT
NIYMTLATLFISQALGVDLSFSQQVTILIVAMLTSKGASGVTGAGFITLAATLSVVNPALVPGMAIVFSIDKFMSEVRAL
TNITGNGIAAVFVSWWEGELDHDLLQRRLNQQIDPSDVETAVTTG
>Mature_444_residues
AAATDIGTRDVPSQTGKKPWYSILYVQVLIAILIGIVVGWLFPNLATNDWIKALGDGFIKLIKMVIAPIIFCTVVSGIAH
IQNASKVGRVGVKALVYFEIVSTFALLLGLIVGNLAPIGHGLAAKPDAAAVANYVKQAEAQKSVDFVLNIIPDSVVGALA
RGDILQVLLFAILFGFALMALGERGHRLRDIIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLIALF
YITAGLFVVIVLGLIARFIGFSIFKFVAYIKDELLIVLGTSSSESALPQLMEKLERLGCSKPVVGLVVPTGYSFNLDGTN
IYMTLATLFISQALGVDLSFSQQVTILIVAMLTSKGASGVTGAGFITLAATLSVVNPALVPGMAIVFSIDKFMSEVRALT
NITGNGIAAVFVSWWEGELDHDLLQRRLNQQIDPSDVETAVTTG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=304, Percent_Identity=31.9078947368421, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=31.7518248175182, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI5032093, Length=421, Percent_Identity=26.603325415677, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI40254478, Length=454, Percent_Identity=24.2290748898678, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI223468564, Length=288, Percent_Identity=28.8194444444444, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI66773030, Length=437, Percent_Identity=24.9427917620137, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI223468566, Length=288, Percent_Identity=28.8194444444444, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI21314632, Length=402, Percent_Identity=24.6268656716418, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI262359914, Length=264, Percent_Identity=30.6818181818182, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194239697, Length=477, Percent_Identity=23.4800838574423, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789947, Length=418, Percent_Identity=56.9377990430622, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790514, Length=410, Percent_Identity=35.609756097561, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1788024, Length=411, Percent_Identity=21.8978102189781, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789473, Length=318, Percent_Identity=22.9559748427673, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17537407, Length=489, Percent_Identity=24.5398773006135, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71996953, Length=444, Percent_Identity=23.4234234234234, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=471, Percent_Identity=24.416135881104, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983106, Length=471, Percent_Identity=24.416135881104, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=467, Percent_Identity=24.1970021413276, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193206654, Length=447, Percent_Identity=24.1610738255034, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=442, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583025, Length=452, Percent_Identity=22.787610619469, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137668, Length=452, Percent_Identity=22.787610619469, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24583023, Length=452, Percent_Identity=22.787610619469, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281360483, Length=468, Percent_Identity=23.9316239316239, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17137666, Length=468, Percent_Identity=23.9316239316239, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281360481, Length=468, Percent_Identity=23.9316239316239, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA2_BRASO (A4YTR6)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001205518.1
- ProteinModelPortal:   A4YTR6
- SMR:   A4YTR6
- STRING:   A4YTR6
- GeneID:   5120739
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO3510
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   GNILCIL
- ProtClustDB:   CLSK818651
- BioCyc:   BSP376:BRADO3510-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 47025; Mature: 46894

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b020ab0)-; HASH(0x1c50f328)-; HASH(0x1c50bdb4)-; HASH(0x1c0d24d0)-; HASH(0x1c50f364)-; HASH(0x1c27e1c4)-; HASH(0x1c50f334)-; HASH(0x1c50bfa0)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAATDIGTRDVPSQTGKKPWYSILYVQVLIAILIGIVVGWLFPNLATNDWIKALGDGFI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KLIKMVIAPIIFCTVVSGIAHIQNASKVGRVGVKALVYFEIVSTFALLLGLIVGNLAPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HGLAAKPDAAAVANYVKQAEAQKSVDFVLNIIPDSVVGALARGDILQVLLFAILFGFALM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGERGHRLRDIIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLIAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
FYITAGLFVVIVLGLIARFIGFSIFKFVAYIKDELLIVLGTSSSESALPQLMEKLERLGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCC
SKPVVGLVVPTGYSFNLDGTNIYMTLATLFISQALGVDLSFSQQVTILIVAMLTSKGASG
CCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
VTGAGFITLAATLSVVNPALVPGMAIVFSIDKFMSEVRALTNITGNGIAAVFVSWWEGEL
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
DHDLLQRRLNQQIDPSDVETAVTTG
CHHHHHHHHCCCCCCCHHHHHHCCH
>Mature Secondary Structure 
AAATDIGTRDVPSQTGKKPWYSILYVQVLIAILIGIVVGWLFPNLATNDWIKALGDGFI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KLIKMVIAPIIFCTVVSGIAHIQNASKVGRVGVKALVYFEIVSTFALLLGLIVGNLAPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HGLAAKPDAAAVANYVKQAEAQKSVDFVLNIIPDSVVGALARGDILQVLLFAILFGFALM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGERGHRLRDIIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLIAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
FYITAGLFVVIVLGLIARFIGFSIFKFVAYIKDELLIVLGTSSSESALPQLMEKLERLGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCC
SKPVVGLVVPTGYSFNLDGTNIYMTLATLFISQALGVDLSFSQQVTILIVAMLTSKGASG
CCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
VTGAGFITLAATLSVVNPALVPGMAIVFSIDKFMSEVRALTNITGNGIAAVFVSWWEGEL
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
DHDLLQRRLNQQIDPSDVETAVTTG
CHHHHHHHHCCCCCCCHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA