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The map label for this gene is amiC [H]

Identifier: 146340472

GI number: 146340472

Start: 3699344

End: 3700600

Strand: Direct

Name: amiC [H]

Synonym: BRADO3513

Alternate gene names: 146340472

Gene position: 3699344-3700600 (Clockwise)

Preceding gene: 146340470

Following gene: 146340473

Centisome position: 49.61

GC content: 68.42

Gene sequence:

>1257_bases
GTGGGTCCAGGGTTCCCGATCGCCACGGACGCACGACTTGCGGGGGACGGCCGGCAAACCCGCTTCGTTCTCGACCTGGA
CAAGACGGTGCCGTTCCGCGCCTTCCTGCTCAGCGATCCGTATCGGGTGGTGGTGGATCTGCCCCAAGTCAGCTTTCATC
TGCCGACCGGAGCCGGGACGGCTGGCCGCGGCTTGGTGAAGGCGTTCCGCTACGGGCTGGTCATGCCTGGTGGCTCCCGA
ATCGTATTCGACCTTACCGGGCCGGCCCGAATCGCGAATTCGACGGTATTGGATGCCGCGAATGGGCAGCCGCCGCGGCT
TGTCATCGAGCTCGAGGAGGCTGACGAGGCGACCTTCGCCCGCGCCGTCGGTGCCGACAGCCGTCTGCAGGCCAAGCCGG
CTGATTCGAGCGCCGCAACCGCCGCTGCGGGCGCCGCACCGGGCGGCGGCCTGCCGCCGTCGCTCAGGCCGCCGTCGACC
ACCGTCGCCGTGGCACCGCCGTCTGCCGGTCCTGCCGATTCCCGGCCCGTGGTGGTCATCGACCCCGGACATGGTGGTAT
CGACTACGGCACCCAGGGCGGCGAGGTTCCGGAGAAGAACCTCGTGCTCGCCTTCGGCCAGGCGCTGCGTGACCGCCTCG
AACGGTCCGGCAAATATCGCGTCGTCATGACCCGCGACGACGACACCTTCATTCCACTCGGCGACCGCGTCCGCATCGCC
CAGAAGCAGTCGGCGGCCCTGTTCGTGTCGGTCCACGCCGACGCACTGCCGCGCGGCGAGGGCAACGCGCAGGGCGCGAC
GATCTATACGCTCTCCGACAAGGCCTCCGACGTCGAGGCGGAGCGGCTGGCCGAGGCGGAGAACCGCGCCGACCAGATCG
GCGGGGTGAACCTGACCGAGGAGCCTGCCGACGTCGCCGACATCCTGATCGACCTCGCCCGGCGCGAAACGCGCACCTTC
TCCAACCGGTTCGCCCGCCTGCTGATGAACGACATGAAGTCCGCTGTCCAAATGCATAAGCGGCCGCTGAAATCCGCCGG
TTTCAGGGTGCTCAAGGCCCCCGATGTGCCCTCCGTTCTGGTCGAACTCGGCTATGTCTCGAACAAGGGCGACATGGGGA
ACCTGGTCTCCGACGCGTGGCGCTCGCGCACAGCGGACGCGATGGCGAAGGCAATCGATGCCTTTCTCGCGAAACGGGTC
GCCAATGCCGGTGGCGAGCGGCCCGAGAAGCCTGCCGCACCGCGTGCAAAGCCCTAG

Upstream 100 bases:

>100_bases
GCTGGGATGCGTGGCGCTCTGCGTCACGCAAATGTTATGTGCGGCGGTCGTCCCGGCCCGGGCACAGAGCGCTCCGGTCG
GCCAGGTCCCTCCTGCTGGC

Downstream 100 bases:

>100_bases
TTTGGCCACAGCCCGACCGGTATAACTTCGCTGCGAGGCACCCGGAATCAGGTGAGAGATTCCTTCTCGGCGGAGTTGTG
GTAGTCTTTGCCCTCGATAG

Product: putative N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGPGFPIATDARLAGDGRQTRFVLDLDKTVPFRAFLLSDPYRVVVDLPQVSFHLPTGAGTAGRGLVKAFRYGLVMPGGSR
IVFDLTGPARIANSTVLDAANGQPPRLVIELEEADEATFARAVGADSRLQAKPADSSAATAAAGAAPGGGLPPSLRPPST
TVAVAPPSAGPADSRPVVVIDPGHGGIDYGTQGGEVPEKNLVLAFGQALRDRLERSGKYRVVMTRDDDTFIPLGDRVRIA
QKQSAALFVSVHADALPRGEGNAQGATIYTLSDKASDVEAERLAEAENRADQIGGVNLTEEPADVADILIDLARRETRTF
SNRFARLLMNDMKSAVQMHKRPLKSAGFRVLKAPDVPSVLVELGYVSNKGDMGNLVSDAWRSRTADAMAKAIDAFLAKRV
ANAGGERPEKPAAPRAKP

Sequences:

>Translated_418_residues
MGPGFPIATDARLAGDGRQTRFVLDLDKTVPFRAFLLSDPYRVVVDLPQVSFHLPTGAGTAGRGLVKAFRYGLVMPGGSR
IVFDLTGPARIANSTVLDAANGQPPRLVIELEEADEATFARAVGADSRLQAKPADSSAATAAAGAAPGGGLPPSLRPPST
TVAVAPPSAGPADSRPVVVIDPGHGGIDYGTQGGEVPEKNLVLAFGQALRDRLERSGKYRVVMTRDDDTFIPLGDRVRIA
QKQSAALFVSVHADALPRGEGNAQGATIYTLSDKASDVEAERLAEAENRADQIGGVNLTEEPADVADILIDLARRETRTF
SNRFARLLMNDMKSAVQMHKRPLKSAGFRVLKAPDVPSVLVELGYVSNKGDMGNLVSDAWRSRTADAMAKAIDAFLAKRV
ANAGGERPEKPAAPRAKP
>Mature_417_residues
GPGFPIATDARLAGDGRQTRFVLDLDKTVPFRAFLLSDPYRVVVDLPQVSFHLPTGAGTAGRGLVKAFRYGLVMPGGSRI
VFDLTGPARIANSTVLDAANGQPPRLVIELEEADEATFARAVGADSRLQAKPADSSAATAAAGAAPGGGLPPSLRPPSTT
VAVAPPSAGPADSRPVVVIDPGHGGIDYGTQGGEVPEKNLVLAFGQALRDRLERSGKYRVVMTRDDDTFIPLGDRVRIAQ
KQSAALFVSVHADALPRGEGNAQGATIYTLSDKASDVEAERLAEAENRADQIGGVNLTEEPADVADILIDLARRETRTFS
NRFARLLMNDMKSAVQMHKRPLKSAGFRVLKAPDVPSVLVELGYVSNKGDMGNLVSDAWRSRTADAMAKAIDAFLAKRVA
NAGGERPEKPAAPRAKP

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=385, Percent_Identity=32.4675324675325, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788776, Length=239, Percent_Identity=37.6569037656904, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=34.061135371179, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44113; Mature: 43982

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPGFPIATDARLAGDGRQTRFVLDLDKTVPFRAFLLSDPYRVVVDLPQVSFHLPTGAGT
CCCCCCCCCCCEECCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCEEEECCCCCCC
AGRGLVKAFRYGLVMPGGSRIVFDLTGPARIANSTVLDAANGQPPRLVIELEEADEATFA
CHHHHHHHHHHCEECCCCCEEEEEECCCHHHCCCEEEECCCCCCCEEEEEECCCCCHHHH
RAVGADSRLQAKPADSSAATAAAGAAPGGGLPPSLRPPSTTVAVAPPSAGPADSRPVVVI
HHHCCCCCEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEE
DPGHGGIDYGTQGGEVPEKNLVLAFGQALRDRLERSGKYRVVMTRDDDTFIPLGDRVRIA
ECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCEEEE
QKQSAALFVSVHADALPRGEGNAQGATIYTLSDKASDVEAERLAEAENRADQIGGVNLTE
ECCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
EPADVADILIDLARRETRTFSNRFARLLMNDMKSAVQMHKRPLKSAGFRVLKAPDVPSVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHH
VELGYVSNKGDMGNLVSDAWRSRTADAMAKAIDAFLAKRVANAGGERPEKPAAPRAKP
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GPGFPIATDARLAGDGRQTRFVLDLDKTVPFRAFLLSDPYRVVVDLPQVSFHLPTGAGT
CCCCCCCCCCEECCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCEEEECCCCCCC
AGRGLVKAFRYGLVMPGGSRIVFDLTGPARIANSTVLDAANGQPPRLVIELEEADEATFA
CHHHHHHHHHHCEECCCCCEEEEEECCCHHHCCCEEEECCCCCCCEEEEEECCCCCHHHH
RAVGADSRLQAKPADSSAATAAAGAAPGGGLPPSLRPPSTTVAVAPPSAGPADSRPVVVI
HHHCCCCCEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEE
DPGHGGIDYGTQGGEVPEKNLVLAFGQALRDRLERSGKYRVVMTRDDDTFIPLGDRVRIA
ECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCEEEE
QKQSAALFVSVHADALPRGEGNAQGATIYTLSDKASDVEAERLAEAENRADQIGGVNLTE
ECCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
EPADVADILIDLARRETRTFSNRFARLLMNDMKSAVQMHKRPLKSAGFRVLKAPDVPSVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHH
VELGYVSNKGDMGNLVSDAWRSRTADAMAKAIDAFLAKRVANAGGERPEKPAAPRAKP
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]