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The map label for this gene is hpcH [H]

Identifier: 146339606

GI number: 146339606

Start: 2782286

End: 2783065

Strand: Direct

Name: hpcH [H]

Synonym: BRADO2598

Alternate gene names: 146339606

Gene position: 2782286-2783065 (Clockwise)

Preceding gene: 146339605

Following gene: 146339607

Centisome position: 37.31

GC content: 62.69

Gene sequence:

>780_bases
GTGGCGAACAACAAGGTCAAGCAGCTCTGGGCCGAAGGCAAGACGGTGGTGAATGCCTGGCTCGCGATTCCCTCGGGGTT
CTCCGCCGAGGTCGTGGCGCAGTGCGGCTTCGACAGCGTCACTGTGGACATGCAGCACGGCGTGCAGGACTATCTCTCGA
TGGTACAATGCTTCCAGGCGATGCACGGCCATCCGGTGACCCCGATGGTCCGCGTGCCGTGGAACGAGCCCGGCATCATC
GGCAAGGTGCTCGACGGCGGCGCCTATGGCGTGATCTGTCCAATGGTCAACACGCCGCAGGAAGCACGCAATCTCGTGTC
CTACTCGAAATATCCGCCGAAGGGCGTGCGTTCGAACGGCCCGATCCGCTCCGGCATGTATGGCAGCGCGGGTGAGTATC
AGAAGACCGCCAATGACGAGATCGTGCTGCTGCCGATGATGGAGACGCGGACGGCGGTCGAGAATATGGAGGCCATTCTC
GATGTCGAGGGCATTGACGGCGTCTATATCGGCCCGTCCGACCTCGGCTTCTCCTACGGCCTCGTGCCGAAGCTCGATCG
CGACGAGCCCGAGATTCTCAAGATCTATGAGAAGATCGTGAAGGAATGCGGCAAGCGCGGCCTCAATCCGGGCATCCATT
GCAGCGGCGCCGAGGGCGCGGTGCGTGCCATCAACATGGGCTTCAAGCTCGTGACACTGTCGAACGAGGTCGGCCTGATG
CAGGTCTATGCCCGCCAGCAGGTGGCGCAGACGCGCAAGGAATCCGGCGGCAAGGCCTGA

Upstream 100 bases:

>100_bases
GTGGCGCGGCGCGGCATGTGAGCGGGATTGCATCGCGCTCACGCATCGAAGAACGAGTTTGAACAACGTAGACTGAACAA
CCAGGGAAGGAAACGAAGTC

Downstream 100 bases:

>100_bases
GGACGAATACCGAAGGGGGAGAGCGAATAGGGGTGTAGATCATCCGCTATTCGCACCTCGCCCATCGCATGTCTGTTTGT
AACTGATGAACTCAACGAGA

Product: putative aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MANNKVKQLWAEGKTVVNAWLAIPSGFSAEVVAQCGFDSVTVDMQHGVQDYLSMVQCFQAMHGHPVTPMVRVPWNEPGII
GKVLDGGAYGVICPMVNTPQEARNLVSYSKYPPKGVRSNGPIRSGMYGSAGEYQKTANDEIVLLPMMETRTAVENMEAIL
DVEGIDGVYIGPSDLGFSYGLVPKLDRDEPEILKIYEKIVKECGKRGLNPGIHCSGAEGAVRAINMGFKLVTLSNEVGLM
QVYARQQVAQTRKESGGKA

Sequences:

>Translated_259_residues
MANNKVKQLWAEGKTVVNAWLAIPSGFSAEVVAQCGFDSVTVDMQHGVQDYLSMVQCFQAMHGHPVTPMVRVPWNEPGII
GKVLDGGAYGVICPMVNTPQEARNLVSYSKYPPKGVRSNGPIRSGMYGSAGEYQKTANDEIVLLPMMETRTAVENMEAIL
DVEGIDGVYIGPSDLGFSYGLVPKLDRDEPEILKIYEKIVKECGKRGLNPGIHCSGAEGAVRAINMGFKLVTLSNEVGLM
QVYARQQVAQTRKESGGKA
>Mature_258_residues
ANNKVKQLWAEGKTVVNAWLAIPSGFSAEVVAQCGFDSVTVDMQHGVQDYLSMVQCFQAMHGHPVTPMVRVPWNEPGIIG
KVLDGGAYGVICPMVNTPQEARNLVSYSKYPPKGVRSNGPIRSGMYGSAGEYQKTANDEIVLLPMMETRTAVENMEAILD
VEGIDGVYIGPSDLGFSYGLVPKLDRDEPEILKIYEKIVKECGKRGLNPGIHCSGAEGAVRAINMGFKLVTLSNEVGLMQ
VYARQQVAQTRKESGGKA

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=252, Percent_Identity=31.7460317460317, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1788578, Length=259, Percent_Identity=30.1158301158301, Blast_Score=130, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28082; Mature: 27950

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNKVKQLWAEGKTVVNAWLAIPSGFSAEVVAQCGFDSVTVDMQHGVQDYLSMVQCFQA
CCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEHHHCHHHHHHHHHHHHH
MHGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMVNTPQEARNLVSYSKYPPKGVRSNG
HCCCCCCCEEECCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCC
PIRSGMYGSAGEYQKTANDEIVLLPMMETRTAVENMEAILDVEGIDGVYIGPSDLGFSYG
CCCCCCCCCCCCCHHCCCCCEEEEECHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCC
LVPKLDRDEPEILKIYEKIVKECGKRGLNPGIHCSGAEGAVRAINMGFKLVTLSNEVGLM
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCEEEEEECCCCCHH
QVYARQQVAQTRKESGGKA
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANNKVKQLWAEGKTVVNAWLAIPSGFSAEVVAQCGFDSVTVDMQHGVQDYLSMVQCFQA
CCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEHHHCHHHHHHHHHHHHH
MHGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMVNTPQEARNLVSYSKYPPKGVRSNG
HCCCCCCCEEECCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCC
PIRSGMYGSAGEYQKTANDEIVLLPMMETRTAVENMEAILDVEGIDGVYIGPSDLGFSYG
CCCCCCCCCCCCCHHCCCCCEEEEECHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCC
LVPKLDRDEPEILKIYEKIVKECGKRGLNPGIHCSGAEGAVRAINMGFKLVTLSNEVGLM
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCEEEEEECCCCCHH
QVYARQQVAQTRKESGGKA
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA