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The map label for this gene is garD [H]

Identifier: 146339607

GI number: 146339607

Start: 2783151

End: 2784698

Strand: Direct

Name: garD [H]

Synonym: BRADO2599

Alternate gene names: 146339607

Gene position: 2783151-2784698 (Clockwise)

Preceding gene: 146339606

Following gene: 146339609

Centisome position: 37.32

GC content: 64.08

Gene sequence:

>1548_bases
ATGAACTCAACGAGAGAGACTGCCATGGCTCCCGCTCCCGTCATCCGGCTTCATCCCGAGGACAGCGTGCTGATCGCGCG
CGCCAGCCTTCCTCCGGGGCTTGCGGTGACGGAGGGAGTCGTGACGGTCGACCGCATACCGGCGGGGCATAAGGTCGCAG
TGCGCCCGATCGCGCAGGGCGAGCCGATTCGGCGCTACGGTCAGATCATCGGCTTCGCCACGGCGCCGATCGCGCCCGGA
CAGCATGTTCATACACAGAACTGCGGCATGGGTGATTTCGCCAAGGACTACGCCTACAGCGTCGACGTCAAGCCGACGCC
GAATTTCGATCTTCCTGCAACCTTCGAGGGCATTCGTCGCCCCGACGGCCGGGTCGCGACCCGCAACTATATCGGCATCC
TGACCTCGGTGAATTGCAGCGCCCATGTCGCTGGTCTTGTGGCCGATGTCTTCAAGCGGAATCCGTTTTCCGGCGACGAT
CCGCTCGCCGACTATCCCAACGTCGACGGCGTGGTGGCGCTGACGCACAAGACCGGCTGCGGCATGACAATGGACGAACC
GCTCAAGCTGTTGCGGCGGACGCTCGGCGGCTACGCGCGGCACGTGAATTTTTCGGCGGTGATCGTGCTCGGTCTCGGCT
GCGAGGCCAATCAGATCGGCGGTCTGATGGAGGAGCAGAAGCTCGCCGGCCGGCTGCGCGCCATGGACATCCAGGAGGTC
GGCGGCACACGCAAGACCGTCGAGGCCGGCATTGCCTTCGTCAAGGAGGCTCTGGCGGATGCCAACAAGGTGAAGCGTGA
GACGGTTCCGGCGAGTGAGCTGACCGTGGCCCTGCAATGTGGCGGCTCCGACGGCTACTCGGGCATCTCGGCCAATCCGG
CGCTCGGTGCCGCGAGCGATCTGCTGGTGCGGCACGGTGGCACGGTGATTTTATCCGAGACACCTGAGACATATGGCGCC
GAGCACCTGCTCACTCGCCGCGCCGTGAGCCGCGAGGTCGGCGAGAAACTCGTCTCTCTGATGCGCTGGTGGGAAGACTA
TACCGCGCGTGAAGGCGCCGAGATGAATGCCAATCCCAGTCCGGGCAACAAGGCCGGGGGTCTCACGACGATTCTCGAAA
AGTCGCTGGGCGCGATGGCCAAGGCGGGCAGCACCAATCTGGTCGACGTGCTGCATTACGCCGAAGCCGTCACCAAGAAG
GGTTTCGTCTTCATGGATACGCCCGGCTACGACCCCGTCGCAGCAACGGGGCAGGTGGCGGGCGGAGCCAATCTCGTCTG
CTTCACCACTGGACGTGGCAGCGTGTTCGGCTGCAAGCCGGCGCCGTCCATCAAGCTTGCAACCAACACGCCGATGTACA
AGCGGATGGAAGACGATATGGACGTCAATTGCGGCACGATCCTGGACGGCGAGGAAAGTGTGCAGGAATGCGGCCAGCGC
ATCTTTGAACTGATGCTGAAGACCGCGTCGGGCGAGCCGACCAAGAGCGAGAGTTTCGATTTCGGCGGCGCCGAGTTCGC
GCCCTGGGTGCTCGGCGCGACCATGTAG

Upstream 100 bases:

>100_bases
GGCGGCAAGGCCTGAGGACGAATACCGAAGGGGGAGAGCGAATAGGGGTGTAGATCATCCGCTATTCGCACCTCGCCCAT
CGCATGTCTGTTTGTAACTG

Downstream 100 bases:

>100_bases
CTCGATCAAGCCGGTCAGTTGCGGAACCGGAATGATGCCGGTCGTATCACGTGCCGGCATCTGATCACAGCTGAGATCGT
CATTTAGCTGAGACTGGATC

Product: putative D-galactarate dehydratase/Altronate hydrolase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNSTRETAMAPAPVIRLHPEDSVLIARASLPPGLAVTEGVVTVDRIPAGHKVAVRPIAQGEPIRRYGQIIGFATAPIAPG
QHVHTQNCGMGDFAKDYAYSVDVKPTPNFDLPATFEGIRRPDGRVATRNYIGILTSVNCSAHVAGLVADVFKRNPFSGDD
PLADYPNVDGVVALTHKTGCGMTMDEPLKLLRRTLGGYARHVNFSAVIVLGLGCEANQIGGLMEEQKLAGRLRAMDIQEV
GGTRKTVEAGIAFVKEALADANKVKRETVPASELTVALQCGGSDGYSGISANPALGAASDLLVRHGGTVILSETPETYGA
EHLLTRRAVSREVGEKLVSLMRWWEDYTAREGAEMNANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLHYAEAVTKK
GFVFMDTPGYDPVAATGQVAGGANLVCFTTGRGSVFGCKPAPSIKLATNTPMYKRMEDDMDVNCGTILDGEESVQECGQR
IFELMLKTASGEPTKSESFDFGGAEFAPWVLGATM

Sequences:

>Translated_515_residues
MNSTRETAMAPAPVIRLHPEDSVLIARASLPPGLAVTEGVVTVDRIPAGHKVAVRPIAQGEPIRRYGQIIGFATAPIAPG
QHVHTQNCGMGDFAKDYAYSVDVKPTPNFDLPATFEGIRRPDGRVATRNYIGILTSVNCSAHVAGLVADVFKRNPFSGDD
PLADYPNVDGVVALTHKTGCGMTMDEPLKLLRRTLGGYARHVNFSAVIVLGLGCEANQIGGLMEEQKLAGRLRAMDIQEV
GGTRKTVEAGIAFVKEALADANKVKRETVPASELTVALQCGGSDGYSGISANPALGAASDLLVRHGGTVILSETPETYGA
EHLLTRRAVSREVGEKLVSLMRWWEDYTAREGAEMNANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLHYAEAVTKK
GFVFMDTPGYDPVAATGQVAGGANLVCFTTGRGSVFGCKPAPSIKLATNTPMYKRMEDDMDVNCGTILDGEESVQECGQR
IFELMLKTASGEPTKSESFDFGGAEFAPWVLGATM
>Mature_515_residues
MNSTRETAMAPAPVIRLHPEDSVLIARASLPPGLAVTEGVVTVDRIPAGHKVAVRPIAQGEPIRRYGQIIGFATAPIAPG
QHVHTQNCGMGDFAKDYAYSVDVKPTPNFDLPATFEGIRRPDGRVATRNYIGILTSVNCSAHVAGLVADVFKRNPFSGDD
PLADYPNVDGVVALTHKTGCGMTMDEPLKLLRRTLGGYARHVNFSAVIVLGLGCEANQIGGLMEEQKLAGRLRAMDIQEV
GGTRKTVEAGIAFVKEALADANKVKRETVPASELTVALQCGGSDGYSGISANPALGAASDLLVRHGGTVILSETPETYGA
EHLLTRRAVSREVGEKLVSLMRWWEDYTAREGAEMNANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLHYAEAVTKK
GFVFMDTPGYDPVAATGQVAGGANLVCFTTGRGSVFGCKPAPSIKLATNTPMYKRMEDDMDVNCGTILDGEESVQECGQR
IFELMLKTASGEPTKSESFDFGGAEFAPWVLGATM

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=492, Percent_Identity=39.8373983739837, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI1789475, Length=508, Percent_Identity=34.4488188976378, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 54540; Mature: 54540

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTRETAMAPAPVIRLHPEDSVLIARASLPPGLAVTEGVVTVDRIPAGHKVAVRPIAQG
CCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCEECCCEEEEECCCCCCEEEEEECCCC
EPIRRYGQIIGFATAPIAPGQHVHTQNCGMGDFAKDYAYSVDVKPTPNFDLPATFEGIRR
CHHHHHHHHHEEEECCCCCCCCEECCCCCCCCHHHHCEEEEECCCCCCCCCCCHHHHHCC
PDGRVATRNYIGILTSVNCSAHVAGLVADVFKRNPFSGDDPLADYPNVDGVVALTHKTGC
CCCCEEECEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCC
GMTMDEPLKLLRRTLGGYARHVNFSAVIVLGLGCEANQIGGLMEEQKLAGRLRAMDIQEV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCHHHH
GGTRKTVEAGIAFVKEALADANKVKRETVPASELTVALQCGGSDGYSGISANPALGAASD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
LLVRHGGTVILSETPETYGAEHLLTRRAVSREVGEKLVSLMRWWEDYTAREGAEMNANPS
HHHHCCCEEEEECCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGNKAGGLTTILEKSLGAMAKAGSTNLVDVLHYAEAVTKKGFVFMDTPGYDPVAATGQVA
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
GGANLVCFTTGRGSVFGCKPAPSIKLATNTPMYKRMEDDMDVNCGTILDGEESVQECGQR
CCCEEEEEECCCCCEEECCCCCCEEEECCCCHHHHHCCCCCCCCCEEECCHHHHHHHHHH
IFELMLKTASGEPTKSESFDFGGAEFAPWVLGATM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MNSTRETAMAPAPVIRLHPEDSVLIARASLPPGLAVTEGVVTVDRIPAGHKVAVRPIAQG
CCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCEECCCEEEEECCCCCCEEEEEECCCC
EPIRRYGQIIGFATAPIAPGQHVHTQNCGMGDFAKDYAYSVDVKPTPNFDLPATFEGIRR
CHHHHHHHHHEEEECCCCCCCCEECCCCCCCCHHHHCEEEEECCCCCCCCCCCHHHHHCC
PDGRVATRNYIGILTSVNCSAHVAGLVADVFKRNPFSGDDPLADYPNVDGVVALTHKTGC
CCCCEEECEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCC
GMTMDEPLKLLRRTLGGYARHVNFSAVIVLGLGCEANQIGGLMEEQKLAGRLRAMDIQEV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCHHHH
GGTRKTVEAGIAFVKEALADANKVKRETVPASELTVALQCGGSDGYSGISANPALGAASD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
LLVRHGGTVILSETPETYGAEHLLTRRAVSREVGEKLVSLMRWWEDYTAREGAEMNANPS
HHHHCCCEEEEECCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGNKAGGLTTILEKSLGAMAKAGSTNLVDVLHYAEAVTKKGFVFMDTPGYDPVAATGQVA
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
GGANLVCFTTGRGSVFGCKPAPSIKLATNTPMYKRMEDDMDVNCGTILDGEESVQECGQR
CCCEEEEEECCCCCEEECCCCCCEEEECCCCHHHHHCCCCCCCCCEEECCHHHHHHHHHH
IFELMLKTASGEPTKSESFDFGGAEFAPWVLGATM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]