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The map label for this gene is ligA [H]

Identifier: 146339394

GI number: 146339394

Start: 2533215

End: 2533568

Strand: Direct

Name: ligA [H]

Synonym: BRADO2379

Alternate gene names: 146339394

Gene position: 2533215-2533568 (Clockwise)

Preceding gene: 146339392

Following gene: 146339395

Centisome position: 33.97

GC content: 63.84

Gene sequence:

>354_bases
ATGTCCGCCACATCGGCCCGGCAGCCTGTGCCCGGCACCACAATCTTCGACGGCGAGCAGGCCCGCAAGGGCTATGCGCT
CAACAAGATGTGCTACTCGTTCAACGACGCCGCCAACCGCAAGGCCTTCGTCGCCGACGAGGACGGCTATTGCAGCAAGT
ATGGTCTCAACGACCAGCAGCGCGCCGCGATCAAGAGCCGCAACGTGCTCGCGCTGATCGAGGCCGGCGGCAACGCCTAT
TACCTCGCCAAGTTCGCCGGCATCTTCGGCCTGGACATGCAGGACATCGGCGCGCAGCAGACCGGACTCACCAAGGATCA
GTTCAAGGCCAAGCTTCTGGCGGCGAGGGGGTAA

Upstream 100 bases:

>100_bases
GATCGGCGGTGTCTCCTGACGATACTGGCGGGCACGGACCACGCCGCGTTTGATCCAGATCAAACGCGGCCCGTCAGAAC
GCTTTCAGGGGAGGGACCCA

Downstream 100 bases:

>100_bases
GCATCATGGCAAAGATCGTCGGCAGCATCACCACGTCTCACGTTCCGGCCATCGGCGGCGCGATCGCCAAGGGGCTGCAG
CAGGATCCGTATTGGAAGCC

Product: protocatechuate 4,5-dioxygenase subunit alpha

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 117; Mature: 116

Protein sequence:

>117_residues
MSATSARQPVPGTTIFDGEQARKGYALNKMCYSFNDAANRKAFVADEDGYCSKYGLNDQQRAAIKSRNVLALIEAGGNAY
YLAKFAGIFGLDMQDIGAQQTGLTKDQFKAKLLAARG

Sequences:

>Translated_117_residues
MSATSARQPVPGTTIFDGEQARKGYALNKMCYSFNDAANRKAFVADEDGYCSKYGLNDQQRAAIKSRNVLALIEAGGNAY
YLAKFAGIFGLDMQDIGAQQTGLTKDQFKAKLLAARG
>Mature_116_residues
SATSARQPVPGTTIFDGEQARKGYALNKMCYSFNDAANRKAFVADEDGYCSKYGLNDQQRAAIKSRNVLALIEAGGNAYY
LAKFAGIFGLDMQDIGAQQTGLTKDQFKAKLLAARG

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014159
- InterPro:   IPR011986 [H]

Pfam domain/function: PF07746 LigA [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 12640; Mature: 12509

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSARQPVPGTTIFDGEQARKGYALNKMCYSFNDAANRKAFVADEDGYCSKYGLNDQQ
CCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHH
RAAIKSRNVLALIEAGGNAYYLAKFAGIFGLDMQDIGAQQTGLTKDQFKAKLLAARG
HHHHCCCCEEEEEECCCCEEEEEEEHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SATSARQPVPGTTIFDGEQARKGYALNKMCYSFNDAANRKAFVADEDGYCSKYGLNDQQ
CCCCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHH
RAAIKSRNVLALIEAGGNAYYLAKFAGIFGLDMQDIGAQQTGLTKDQFKAKLLAARG
HHHHCCCCEEEEEECCCCEEEEEEEHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]