Click here to switch to the map view.

The map label for this gene is mhpB [H]

Identifier: 146339395

GI number: 146339395

Start: 2533574

End: 2534410

Strand: Direct

Name: mhpB [H]

Synonym: BRADO2380

Alternate gene names: 146339395

Gene position: 2533574-2534410 (Clockwise)

Preceding gene: 146339394

Following gene: 146339403

Centisome position: 33.98

GC content: 64.16

Gene sequence:

>837_bases
ATGGCAAAGATCGTCGGCAGCATCACCACGTCTCACGTTCCGGCCATCGGCGGCGCGATCGCCAAGGGGCTGCAGCAGGA
TCCGTATTGGAAGCCGTTCTTCGACGGCTTTCCGCACGTCCGCGACTGGCTCGCCAAGGTGAAGCCGGATGTCGTCGTGC
TCGTTTACAACGATCACGGCCTCAATTTCTTCCTCGACAAGATGCCGACCTTTGCGATCGGCGCCTGCGAGAGCTACAGC
AATGCCGACGAGGGCTGGGGCATTCCGCAGACCGCGCCGTTCAAGGGCGACAGCGAATTGTCCTGGCACCTGATCGAGCA
TCTGGTGGGAGAGGATTTCGACCTCACCACCTGCCAGGAGATGGTCGTCGATCACGCCTTCACCTTGCCGATGGCGCTGT
GCTGGCCGGACCAGAAATGGCCGGTACGCGTGGTGCCGGTGGTCGTCAACACCGTGCAGGCGCCGCTGCCCTCGGCCGCG
CGCTGCTACAAGCTCGGACAGGCGCTGGCGCGCGCGATCGAGTCCTGGCCGAAGGACGAGCGCGTGGTGGTGATGGGCAC
CGGCGGCCTGTCGCATCAGCTCGACGGCGAGCGGGCGGGATTCATCAACAAGGACTTCGACGAGAAATTCATGGCCAGCA
TGGTCGACGACCCGGCCTGGGCGACGAAGTACTCCACGACCGAGCTCGTCGAGCTGTCGGGCACGCAGGGCGTCGAGCTG
CTCAACTGGATGGTCGCGCGCGGCGCGCTGCCGGAGAAGGTCCGCAAGGAGCATGCGAACTATCACATTCCGATCTCCAA
CACGGCGACCGGATTGATGGTGCTGGAGCCGGTGTAA

Upstream 100 bases:

>100_bases
GCATCTTCGGCCTGGACATGCAGGACATCGGCGCGCAGCAGACCGGACTCACCAAGGATCAGTTCAAGGCCAAGCTTCTG
GCGGCGAGGGGGTAAGCATC

Downstream 100 bases:

>100_bases
CGGCTGCTCCAGGATTTGTTTTCTGGATGGCGATTACAGCGAGCTGTTCCGCCAATGACCATCACGCGGTATGGGTCCCG
GCTCAAGGCCGGGACGACAG

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MAKIVGSITTSHVPAIGGAIAKGLQQDPYWKPFFDGFPHVRDWLAKVKPDVVVLVYNDHGLNFFLDKMPTFAIGACESYS
NADEGWGIPQTAPFKGDSELSWHLIEHLVGEDFDLTTCQEMVVDHAFTLPMALCWPDQKWPVRVVPVVVNTVQAPLPSAA
RCYKLGQALARAIESWPKDERVVVMGTGGLSHQLDGERAGFINKDFDEKFMASMVDDPAWATKYSTTELVELSGTQGVEL
LNWMVARGALPEKVRKEHANYHIPISNTATGLMVLEPV

Sequences:

>Translated_278_residues
MAKIVGSITTSHVPAIGGAIAKGLQQDPYWKPFFDGFPHVRDWLAKVKPDVVVLVYNDHGLNFFLDKMPTFAIGACESYS
NADEGWGIPQTAPFKGDSELSWHLIEHLVGEDFDLTTCQEMVVDHAFTLPMALCWPDQKWPVRVVPVVVNTVQAPLPSAA
RCYKLGQALARAIESWPKDERVVVMGTGGLSHQLDGERAGFINKDFDEKFMASMVDDPAWATKYSTTELVELSGTQGVEL
LNWMVARGALPEKVRKEHANYHIPISNTATGLMVLEPV
>Mature_277_residues
AKIVGSITTSHVPAIGGAIAKGLQQDPYWKPFFDGFPHVRDWLAKVKPDVVVLVYNDHGLNFFLDKMPTFAIGACESYSN
ADEGWGIPQTAPFKGDSELSWHLIEHLVGEDFDLTTCQEMVVDHAFTLPMALCWPDQKWPVRVVPVVVNTVQAPLPSAAR
CYKLGQALARAIESWPKDERVVVMGTGGLSHQLDGERAGFINKDFDEKFMASMVDDPAWATKYSTTELVELSGTQGVELL
NWMVARGALPEKVRKEHANYHIPISNTATGLMVLEPV

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 30734; Mature: 30603

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVGSITTSHVPAIGGAIAKGLQQDPYWKPFFDGFPHVRDWLAKVKPDVVVLVYNDHG
CCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHCCCCEEEEEECCCC
LNFFLDKMPTFAIGACESYSNADEGWGIPQTAPFKGDSELSWHLIEHLVGEDFDLTTCQE
CEEHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
MVVDHAFTLPMALCWPDQKWPVRVVPVVVNTVQAPLPSAARCYKLGQALARAIESWPKDE
HHHHHHHHCCEEEECCCCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
RVVVMGTGGLSHQLDGERAGFINKDFDEKFMASMVDDPAWATKYSTTELVELSGTQGVEL
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCHHH
LNWMVARGALPEKVRKEHANYHIPISNTATGLMVLEPV
HHHHHHCCCCHHHHHHHCCCEEEEECCCCCCEEEEECC
>Mature Secondary Structure 
AKIVGSITTSHVPAIGGAIAKGLQQDPYWKPFFDGFPHVRDWLAKVKPDVVVLVYNDHG
CCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHCCCCEEEEEECCCC
LNFFLDKMPTFAIGACESYSNADEGWGIPQTAPFKGDSELSWHLIEHLVGEDFDLTTCQE
CEEHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
MVVDHAFTLPMALCWPDQKWPVRVVPVVVNTVQAPLPSAARCYKLGQALARAIESWPKDE
HHHHHHHHCCEEEECCCCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
RVVVMGTGGLSHQLDGERAGFINKDFDEKFMASMVDDPAWATKYSTTELVELSGTQGVEL
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCHHH
LNWMVARGALPEKVRKEHANYHIPISNTATGLMVLEPV
HHHHHHCCCCHHHHHHHCCCEEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]