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The map label for this gene is pps [H]

Identifier: 146338642

GI number: 146338642

Start: 1684465

End: 1686900

Strand: Reverse

Name: pps [H]

Synonym: BRADO1573

Alternate gene names: 146338642

Gene position: 1686900-1684465 (Counterclockwise)

Preceding gene: 146338644

Following gene: 146338638

Centisome position: 22.62

GC content: 67.24

Gene sequence:

>2436_bases
ATGAATGCATCCGGTTATATTCGCTGGTTCAAGGACCTTCGCATCGAGGACGTCCCCGACGTCGGCGGCAAGACCGCGTC
GCTCGGGGAGCTGTATTCGGCGCTGGCGGCGCAGGGCGTACGCGTGCCGAACGGCTTCGCCTTGACCGCGGCAAGCTATC
GCGATGCGCTGCGCGCCGACGGCGCCGACGATGCGTTGCGTGAGCTGCTCGCCGGACTCGACAAACGCCGCATCGCCGAT
CTCGCCAAGCGAGCGGCCAAGGCGCGCGCGATCGTGTTCGACGCCACCGATCGTGACGATCTCCGGCGCGAGGTGGCCAT
GGCGTACAAGCAGCTCGAGCAGGAATATGGCCGCAACGTCGCGGTGGCCGTGCGCAGTTCGGCGACCGCCGAGGACCTGC
CCCATGCGAGCTTCGCCGGCCAGCACGAGAGCTTCCTCAACATCAGCGGCGCCAGGCAGCTGTTCGAGACCTGCCGCCGC
TGCTTCGCCTCGCTCTACACCGATCGCGCGATCTCCTACCGCATCGACAATGGCTTCGCCCACGACAAGGTCGCGCTGTC
GGTGGCCGTGATGAAGATGGTGCGCTCCGATCGCGGCGCCAGCGGCGTGATGTTCTCGCTGGACACCGAGAGCGGCTTTC
GCGACGTCGCCTTCATCACCGGCGTCTACGGGCTCGGCGAGACCATCGTGCAGGGCGCGGTCGATCCCGACGAGTTCTAC
GTCCACAAGCCGACCTTCGCCGCCGGCTTCCGCAGCGTGCTGTCGCGGCGGCTCGGCGCCAAGAGCAAGCGTATGGTCTA
TGCGAAGCCGGGCGCGCGCGGCGCCACCACGCGCATCGAGCCGACCCCGCCGGCCGAGCGCGCCAGGTTCTGTATCGACG
ACTCCGACGTGCTGGAGCTGGCGCAGGCGACGATCACCGTCGAGCAGCACTACTCGCAGAAGGCCGGCCGGCCGACGCCG
ATGGACATCGAATGGGCCAAGGACGGCCGCGACGGCGCGCTCTACATCATCCAGGCCCGGCCCGAGACGGTGGCCTCGCA
GCGGCGGCCGGAGTCGGTCGAGACCTATGTGCTGAAAAAGGCCGGCAAGCCGCTCGCGACCGGCCGCGCCGTCGGCGAGA
AGATCGCCGCAGGCAAGGTCCGCCTGATCAGAACGGCAACGGACCTGCGCGCGTTCAAGCCCGGCGAGGTGCTAGTGGCC
ACCTCGACCAGCCCGGATTGGGAGCCCGTCATGAAGACCGCGGCGGCGATCGTCACGGATCATGGCGGCCGGACCTGCCA
TGCCGCGATCATCGCGCGCGAACTCGGCGTGCCCGCGGTGGTCGGCACCGAGGATTTCAGCAAGCGCGCCAGGACGGGCG
CGAGCGTCACCGTGTGCTGCGCCGAGGGCGATGCCGGACATGTCTATGACGGCGCCCTGCCGTTCGACGTCCAGCGTCTG
GCGATCGACAAGCTCGACAAGCCGCACACCCACATCATGGTCAATCTCGGCAATCCCGAGCTCGCCTTCAAGACCGCACT
CTTGCCGAACGACGGCGTCGGCCTCGCACGCATGGAGTTCATCATCAACCAGTCGATCGGCATTCATCCGATGGCATTGG
CTGCGCCGAACAAGGTGACCGCGCCAAAGGACCGCGCGACGATCAAGCAACTGACCGCCCGCTACAGGAAGCCCGCCGAC
TTCTTCGTGGAGCAGCTGTCGGAAGGGGTCGGCACCATCGCGGCCGCCTTCTATCCGCGCCCGGTCATCATCAGGCTGTC
GGACTTCAAGACCAACGAATATGCCAGCCTGATTGGCGGCGCCGACTTCGAGCCCAGGGAGGAGAACCCGATGCTCGGCT
TCCGCGGCGCCTCGCGCTATGCGCATCCGGCCTATGCCGCCGGCTTCGCGCTGGAATGCGCCGCCCTGAAGCGCGTCCGC
GACGTCATGGGCCTCGCCAATCTGAAGATCATGATCCCGTTCTGCCGCCGCGTTGCGGAGGCGGAGAAGGTGATCGCGGC
GATGGCCGGGCACGGGCTCGTCCGCGGCGAGCACGGATTGGAGATCTACATGATGTGCGAGATCCCCAACAACGTCATCC
TGATCGACGAGTTCGCGAAACATTTCGACGGCTTCTCGATCGGCTCCAACGACCTGACCCAGCTCACGCTCGGCGTCGAC
CGCGATTCCGACATCGTCGCGTTCGATTTCGACGAGCGCGATCCCGGTCTCCTGACCATGCTCAAGCTCGCGGTGGAGGG
CGCGCATCGCAACCACCGCCATGTCGGCATCTGCGGCGAGGCGCCGGCCAACTATCCGGAGGTGGCACGCTATCTGGCGA
GCCTCGGCATCGATTCGATCAGCGTCAACCCGGCCAGCGTCATGCGGACGATGGAGATCGTGCGCGTCGCGGAGCGGGAC
GCCGCAGCCAGCGTGCCGCAGGCGCTGGCGAGCTAG

Upstream 100 bases:

>100_bases
ATCTAAGGAGCCACGCACATGCCCCGCTTGATCTTGCGCAAGCCGGCTGCCGTCCGGCGCGGCAAACTCGGCTCTCGCGC
TGCGATGCGGGAGACACGCC

Downstream 100 bases:

>100_bases
TCCAGATCGTGGTCCCTGCCGGACGTGGCGCCGTGCTGGACCAGCGCGATGGCCCGTTCGGCGTCGGCGATCAGTTGCCG
GATATGGCGCGACTGCTCGG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MNASGYIRWFKDLRIEDVPDVGGKTASLGELYSALAAQGVRVPNGFALTAASYRDALRADGADDALRELLAGLDKRRIAD
LAKRAAKARAIVFDATDRDDLRREVAMAYKQLEQEYGRNVAVAVRSSATAEDLPHASFAGQHESFLNISGARQLFETCRR
CFASLYTDRAISYRIDNGFAHDKVALSVAVMKMVRSDRGASGVMFSLDTESGFRDVAFITGVYGLGETIVQGAVDPDEFY
VHKPTFAAGFRSVLSRRLGAKSKRMVYAKPGARGATTRIEPTPPAERARFCIDDSDVLELAQATITVEQHYSQKAGRPTP
MDIEWAKDGRDGALYIIQARPETVASQRRPESVETYVLKKAGKPLATGRAVGEKIAAGKVRLIRTATDLRAFKPGEVLVA
TSTSPDWEPVMKTAAAIVTDHGGRTCHAAIIARELGVPAVVGTEDFSKRARTGASVTVCCAEGDAGHVYDGALPFDVQRL
AIDKLDKPHTHIMVNLGNPELAFKTALLPNDGVGLARMEFIINQSIGIHPMALAAPNKVTAPKDRATIKQLTARYRKPAD
FFVEQLSEGVGTIAAAFYPRPVIIRLSDFKTNEYASLIGGADFEPREENPMLGFRGASRYAHPAYAAGFALECAALKRVR
DVMGLANLKIMIPFCRRVAEAEKVIAAMAGHGLVRGEHGLEIYMMCEIPNNVILIDEFAKHFDGFSIGSNDLTQLTLGVD
RDSDIVAFDFDERDPGLLTMLKLAVEGAHRNHRHVGICGEAPANYPEVARYLASLGIDSISVNPASVMRTMEIVRVAERD
AAASVPQALAS

Sequences:

>Translated_811_residues
MNASGYIRWFKDLRIEDVPDVGGKTASLGELYSALAAQGVRVPNGFALTAASYRDALRADGADDALRELLAGLDKRRIAD
LAKRAAKARAIVFDATDRDDLRREVAMAYKQLEQEYGRNVAVAVRSSATAEDLPHASFAGQHESFLNISGARQLFETCRR
CFASLYTDRAISYRIDNGFAHDKVALSVAVMKMVRSDRGASGVMFSLDTESGFRDVAFITGVYGLGETIVQGAVDPDEFY
VHKPTFAAGFRSVLSRRLGAKSKRMVYAKPGARGATTRIEPTPPAERARFCIDDSDVLELAQATITVEQHYSQKAGRPTP
MDIEWAKDGRDGALYIIQARPETVASQRRPESVETYVLKKAGKPLATGRAVGEKIAAGKVRLIRTATDLRAFKPGEVLVA
TSTSPDWEPVMKTAAAIVTDHGGRTCHAAIIARELGVPAVVGTEDFSKRARTGASVTVCCAEGDAGHVYDGALPFDVQRL
AIDKLDKPHTHIMVNLGNPELAFKTALLPNDGVGLARMEFIINQSIGIHPMALAAPNKVTAPKDRATIKQLTARYRKPAD
FFVEQLSEGVGTIAAAFYPRPVIIRLSDFKTNEYASLIGGADFEPREENPMLGFRGASRYAHPAYAAGFALECAALKRVR
DVMGLANLKIMIPFCRRVAEAEKVIAAMAGHGLVRGEHGLEIYMMCEIPNNVILIDEFAKHFDGFSIGSNDLTQLTLGVD
RDSDIVAFDFDERDPGLLTMLKLAVEGAHRNHRHVGICGEAPANYPEVARYLASLGIDSISVNPASVMRTMEIVRVAERD
AAASVPQALAS
>Mature_811_residues
MNASGYIRWFKDLRIEDVPDVGGKTASLGELYSALAAQGVRVPNGFALTAASYRDALRADGADDALRELLAGLDKRRIAD
LAKRAAKARAIVFDATDRDDLRREVAMAYKQLEQEYGRNVAVAVRSSATAEDLPHASFAGQHESFLNISGARQLFETCRR
CFASLYTDRAISYRIDNGFAHDKVALSVAVMKMVRSDRGASGVMFSLDTESGFRDVAFITGVYGLGETIVQGAVDPDEFY
VHKPTFAAGFRSVLSRRLGAKSKRMVYAKPGARGATTRIEPTPPAERARFCIDDSDVLELAQATITVEQHYSQKAGRPTP
MDIEWAKDGRDGALYIIQARPETVASQRRPESVETYVLKKAGKPLATGRAVGEKIAAGKVRLIRTATDLRAFKPGEVLVA
TSTSPDWEPVMKTAAAIVTDHGGRTCHAAIIARELGVPAVVGTEDFSKRARTGASVTVCCAEGDAGHVYDGALPFDVQRL
AIDKLDKPHTHIMVNLGNPELAFKTALLPNDGVGLARMEFIINQSIGIHPMALAAPNKVTAPKDRATIKQLTARYRKPAD
FFVEQLSEGVGTIAAAFYPRPVIIRLSDFKTNEYASLIGGADFEPREENPMLGFRGASRYAHPAYAAGFALECAALKRVR
DVMGLANLKIMIPFCRRVAEAEKVIAAMAGHGLVRGEHGLEIYMMCEIPNNVILIDEFAKHFDGFSIGSNDLTQLTLGVD
RDSDIVAFDFDERDPGLLTMLKLAVEGAHRNHRHVGICGEAPANYPEVARYLASLGIDSISVNPASVMRTMEIVRVAERD
AAASVPQALAS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=788, Percent_Identity=50.3807106598985, Blast_Score=805, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=457, Percent_Identity=25.382932166302, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=33.1578947368421, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=32.9842931937173, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1789193, Length=423, Percent_Identity=26.0047281323877, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=320, Percent_Identity=23.4375, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87985; Mature: 87985

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASGYIRWFKDLRIEDVPDVGGKTASLGELYSALAAQGVRVPNGFALTAASYRDALRAD
CCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCC
GADDALRELLAGLDKRRIADLAKRAAKARAIVFDATDRDDLRREVAMAYKQLEQEYGRNV
CHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEE
AVAVRSSATAEDLPHASFAGQHESFLNISGARQLFETCRRCFASLYTDRAISYRIDNGFA
EEEEECCCCHHHCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
HDKVALSVAVMKMVRSDRGASGVMFSLDTESGFRDVAFITGVYGLGETIVQGAVDPDEFY
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
VHKPTFAAGFRSVLSRRLGAKSKRMVYAKPGARGATTRIEPTPPAERARFCIDDSDVLEL
EECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHCEEECCCHHHHHH
AQATITVEQHYSQKAGRPTPMDIEWAKDGRDGALYIIQARPETVASQRRPESVETYVLKK
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHH
AGKPLATGRAVGEKIAAGKVRLIRTATDLRAFKPGEVLVATSTSPDWEPVMKTAAAIVTD
CCCCCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
HGGRTCHAAIIARELGVPAVVGTEDFSKRARTGASVTVCCAEGDAGHVYDGALPFDVQRL
CCCCCHHHHHHHHHCCCCEEECCHHHHHHHCCCCCEEEEEECCCCCCEECCCCCCHHHHH
AIDKLDKPHTHIMVNLGNPELAFKTALLPNDGVGLARMEFIINQSIGIHPMALAAPNKVT
HHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCC
APKDRATIKQLTARYRKPADFFVEQLSEGVGTIAAAFYPRPVIIRLSDFKTNEYASLIGG
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCC
ADFEPREENPMLGFRGASRYAHPAYAAGFALECAALKRVRDVMGLANLKIMIPFCRRVAE
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
AEKVIAAMAGHGLVRGEHGLEIYMMCEIPNNVILIDEFAKHFDGFSIGSNDLTQLTLGVD
HHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCEEEEEECC
RDSDIVAFDFDERDPGLLTMLKLAVEGAHRNHRHVGICGEAPANYPEVARYLASLGIDSI
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCC
SVNPASVMRTMEIVRVAERDAAASVPQALAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNASGYIRWFKDLRIEDVPDVGGKTASLGELYSALAAQGVRVPNGFALTAASYRDALRAD
CCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCC
GADDALRELLAGLDKRRIADLAKRAAKARAIVFDATDRDDLRREVAMAYKQLEQEYGRNV
CHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEE
AVAVRSSATAEDLPHASFAGQHESFLNISGARQLFETCRRCFASLYTDRAISYRIDNGFA
EEEEECCCCHHHCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
HDKVALSVAVMKMVRSDRGASGVMFSLDTESGFRDVAFITGVYGLGETIVQGAVDPDEFY
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
VHKPTFAAGFRSVLSRRLGAKSKRMVYAKPGARGATTRIEPTPPAERARFCIDDSDVLEL
EECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHCEEECCCHHHHHH
AQATITVEQHYSQKAGRPTPMDIEWAKDGRDGALYIIQARPETVASQRRPESVETYVLKK
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHH
AGKPLATGRAVGEKIAAGKVRLIRTATDLRAFKPGEVLVATSTSPDWEPVMKTAAAIVTD
CCCCCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
HGGRTCHAAIIARELGVPAVVGTEDFSKRARTGASVTVCCAEGDAGHVYDGALPFDVQRL
CCCCCHHHHHHHHHCCCCEEECCHHHHHHHCCCCCEEEEEECCCCCCEECCCCCCHHHHH
AIDKLDKPHTHIMVNLGNPELAFKTALLPNDGVGLARMEFIINQSIGIHPMALAAPNKVT
HHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCC
APKDRATIKQLTARYRKPADFFVEQLSEGVGTIAAAFYPRPVIIRLSDFKTNEYASLIGG
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCC
ADFEPREENPMLGFRGASRYAHPAYAAGFALECAALKRVRDVMGLANLKIMIPFCRRVAE
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
AEKVIAAMAGHGLVRGEHGLEIYMMCEIPNNVILIDEFAKHFDGFSIGSNDLTQLTLGVD
HHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCEEEEEECC
RDSDIVAFDFDERDPGLLTMLKLAVEGAHRNHRHVGICGEAPANYPEVARYLASLGIDSI
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCC
SVNPASVMRTMEIVRVAERDAAASVPQALAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]