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The map label for this gene is pfkB [H]

Identifier: 146338644

GI number: 146338644

Start: 1688145

End: 1689083

Strand: Reverse

Name: pfkB [H]

Synonym: BRADO1575

Alternate gene names: 146338644

Gene position: 1689083-1688145 (Counterclockwise)

Preceding gene: 146338645

Following gene: 146338642

Centisome position: 22.65

GC content: 70.18

Gene sequence:

>939_bases
ATGAGCGACATCGTCACGATCACGCCGAACCCGGCCGTCGATGTCTCGACGACCGTCGAGAGCATCGTGCCCGTGGCCAA
GCTGCGCGGCACCACGCAGCGGCGCGATCCCGGCGGCGGCGGCATCAATGTCGCACGCGTCATCACGCGGCTCGGCGGCG
ACGTGACGGCGCTCTATCCAGTGGGCGGCCCAACCGGCCTCCTGCTGAAGCAATTGGTCGAGAGCGAAGGCGTGGCCAGC
CGCACCTGGGCCACGCATGACGACACGCGCGAGGATTTCTTTGTCCAGGAGCGCGCCACCGGCCAGCCTTATCGCTTCAT
CCTGCCGGGACCGACGCTCAGCGAAGCCGAGTCGCAGGCCGGGCTCGCGCTGCTCAGCGGACTCGATCCGTTCCCGCGTT
ACGTCATCGGCAGCGGCAGCCTGCCGCGCGGCGTGCCGACGGACTACTACGCGCGCATCGCGAAGATCGCGCGCGCACGC
GGCGCGCAGTTCATCCTCGATACATCCGGACCTGCGCTGGCCGCGGCGGTCGAAGGCGGCGTCGACCTGATCAAGCCGTC
GCTGCGCGAGATGCACGAACTCGTCGGCGGCGAGCTGCCTGATGCGGTGGCGTGGGAAGCCGCCGCGCGAACGCTGGTTG
CGACAGGCAAGGCCAGGATCGTCGCGCTGACCATGGGCCATCTCGGCGCGCTCGTGGTGACATCCGAGCGCGTGCTGCGT
GCGCAGCCGCTGACGCTGGTGCCGGTCAACGCTGTCGGCGCCGGCGACAGTTTTCTCGGCGCCTTGATCGCCAAGCTCGC
CGCCGGTGAGCCCATGGACGAGGCGTTCCGCTTCGCCGTCGCCGCCGGCGCGGCCGCACTGCTGCGCACGGGCACGGCGC
TGTGCGACCCGGCTGATATCAACCGTCTGCTGGCTCACGTGCGGCTGCAGACGGCGTGA

Upstream 100 bases:

>100_bases
CGTACATCTTGTCACCAAGCCCGACATCAGCTTTCTCGGCCTCAAGTCCTTCCACGAGTCCAGCGTCCCCTCGAGCGGCG
GATCTCCCGCAACCTGATCG

Downstream 100 bases:

>100_bases
CCGTCTCGACGCCATCGTCATTCAGCTCATCGAGCGACAGCAGCAATCCGCCGACGCGCTCCAGCGACGACATCCAGTCG
GCGACGCGGCTGTAATTGTC

Product: carbohydrate kinase

Products: ADP; D-fructose 1,6-bisphosphate

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSDIVTITPNPAVDVSTTVESIVPVAKLRGTTQRRDPGGGGINVARVITRLGGDVTALYPVGGPTGLLLKQLVESEGVAS
RTWATHDDTREDFFVQERATGQPYRFILPGPTLSEAESQAGLALLSGLDPFPRYVIGSGSLPRGVPTDYYARIAKIARAR
GAQFILDTSGPALAAAVEGGVDLIKPSLREMHELVGGELPDAVAWEAAARTLVATGKARIVALTMGHLGALVVTSERVLR
AQPLTLVPVNAVGAGDSFLGALIAKLAAGEPMDEAFRFAVAAGAAALLRTGTALCDPADINRLLAHVRLQTA

Sequences:

>Translated_312_residues
MSDIVTITPNPAVDVSTTVESIVPVAKLRGTTQRRDPGGGGINVARVITRLGGDVTALYPVGGPTGLLLKQLVESEGVAS
RTWATHDDTREDFFVQERATGQPYRFILPGPTLSEAESQAGLALLSGLDPFPRYVIGSGSLPRGVPTDYYARIAKIARAR
GAQFILDTSGPALAAAVEGGVDLIKPSLREMHELVGGELPDAVAWEAAARTLVATGKARIVALTMGHLGALVVTSERVLR
AQPLTLVPVNAVGAGDSFLGALIAKLAAGEPMDEAFRFAVAAGAAALLRTGTALCDPADINRLLAHVRLQTA
>Mature_311_residues
SDIVTITPNPAVDVSTTVESIVPVAKLRGTTQRRDPGGGGINVARVITRLGGDVTALYPVGGPTGLLLKQLVESEGVASR
TWATHDDTREDFFVQERATGQPYRFILPGPTLSEAESQAGLALLSGLDPFPRYVIGSGSLPRGVPTDYYARIAKIARARG
AQFILDTSGPALAAAVEGGVDLIKPSLREMHELVGGELPDAVAWEAAARTLVATGKARIVALTMGHLGALVVTSERVLRA
QPLTLVPVNAVGAGDSFLGALIAKLAAGEPMDEAFRFAVAAGAAALLRTGTALCDPADINRLLAHVRLQTA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=314, Percent_Identity=40.4458598726115, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1788493, Length=312, Percent_Identity=31.0897435897436, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11

Molecular weight: Translated: 32455; Mature: 32323

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIVTITPNPAVDVSTTVESIVPVAKLRGTTQRRDPGGGGINVARVITRLGGDVTALYP
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
VGGPTGLLLKQLVESEGVASRTWATHDDTREDFFVQERATGQPYRFILPGPTLSEAESQA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEHHCCCCCCEEEEECCCCHHHHHHHC
GLALLSGLDPFPRYVIGSGSLPRGVPTDYYARIAKIARARGAQFILDTSGPALAAAVEGG
CHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHHCC
VDLIKPSLREMHELVGGELPDAVAWEAAARTLVATGKARIVALTMGHLGALVVTSERVLR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCEEEEEEECCCCCEEEEEHHHHHH
AQPLTLVPVNAVGAGDSFLGALIAKLAAGEPMDEAFRFAVAAGAAALLRTGTALCDPADI
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NRLLAHVRLQTA
HHHHHHHHHCCC
>Mature Secondary Structure 
SDIVTITPNPAVDVSTTVESIVPVAKLRGTTQRRDPGGGGINVARVITRLGGDVTALYP
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
VGGPTGLLLKQLVESEGVASRTWATHDDTREDFFVQERATGQPYRFILPGPTLSEAESQA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEHHCCCCCCEEEEECCCCHHHHHHHC
GLALLSGLDPFPRYVIGSGSLPRGVPTDYYARIAKIARARGAQFILDTSGPALAAAVEGG
CHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHHCC
VDLIKPSLREMHELVGGELPDAVAWEAAARTLVATGKARIVALTMGHLGALVVTSERVLR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCEEEEEEECCCCCEEEEEHHHHHH
AQPLTLVPVNAVGAGDSFLGALIAKLAAGEPMDEAFRFAVAAGAAALLRTGTALCDPADI
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NRLLAHVRLQTA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose 6-phosphate

Specific reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]