Click here to switch to the map view.

The map label for this gene is ynfL [H]

Identifier: 146338637

GI number: 146338637

Start: 1677420

End: 1678322

Strand: Reverse

Name: ynfL [H]

Synonym: BRADO1566

Alternate gene names: 146338637

Gene position: 1678322-1677420 (Counterclockwise)

Preceding gene: 146338638

Following gene: 146338636

Centisome position: 22.51

GC content: 69.32

Gene sequence:

>903_bases
ATGTTCGATCTCAACCAGCTGCGCTGCTTCGTCACCGTGGCCGAGGAGCTGCATTTCGGGCGCGCGGCCGTGCGGCTGAA
CATGACGCAGCCGCCGCTGTCGCGGCAGATCCAGGTGCTGGAGCACATCATCGATGCCGCGCTGCTGGAACGCACCAGCC
GCTCGGTGAAGCTGACGCCGGCCGGGCGCAGCTTCCTGCCTGAGGCGCGGCGCATCCTGAAGATGGCGGAAAGCGCCGCG
CAGGTGGCGCAGCGCATCGCCAGCGGCAAGAGCGGCTCGCTCAAGATCGGCTTCACCGCGGCCGCCGCCTATGGCTTCCT
GCCCGACCTCGTCGCCGCCTGCCGCAGCGAGCTGCCCGAGGCCGACCTGTCGCTGAAGGAGATGGTGTCGGGCGACCAGA
TCGAGGCACTCGCCTCCGGCCAGATCGATGCCGGCCTGCTGCGGCCGCCGATCGCGCGGCCTGAATTCGCCACCCGGCGC
GTCGTCGCCGAGCCGCTCTGCGCCGCCGTGCCGCGCCGGCATCGGCTGGCCTCCGCCGACGCCGTGTCGATCCGCGATTT
CGACGGCCAGCCCTTCATCATGTATTCGCAGACCGAGGCGCGCTATTTTCATGATCTCCTGGTGTCGCAGTTCATGCGCG
CCGAGGTGCTGCCGCGCTACGTGCAGCATCTCAGCCAGATCCATTCCATCCTCGCGATGGTACGCGCCGGCCTGGGGCTT
GCAATCGTGCCGGCCGCTGCCGCGAGCCTCAAGATCGCCGACGTCCGGCTGCGGCCGCTCAAGCTGCGCAATCCAACGCC
GGCCGAGCTGTTCCTGGTCTGGCGGCGCGAGCACGACAATCCGCTGCTGCCGGCGCTGGTCGAGCTGGCCGGCCGCCTTG
CGGTGCAACAGACGGAAGATTGA

Upstream 100 bases:

>100_bases
CCGGGCCATAACCAGACAGCCGGTCGCTGCCGTTCCCAACGCGTCAGATCGATGCTACCAACGCCGCAATCATCGCCCCA
TCCATACCCTGGAAGCATCA

Downstream 100 bases:

>100_bases
TACGCCATCCGTATCAATCGATACCGTCATTGGCTTGGACCGGCATCGAACTTGGTCCTACGACAGCGCCGTGAGGAATG
CCCGCTGTGCAATCGCCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MFDLNQLRCFVTVAEELHFGRAAVRLNMTQPPLSRQIQVLEHIIDAALLERTSRSVKLTPAGRSFLPEARRILKMAESAA
QVAQRIASGKSGSLKIGFTAAAAYGFLPDLVAACRSELPEADLSLKEMVSGDQIEALASGQIDAGLLRPPIARPEFATRR
VVAEPLCAAVPRRHRLASADAVSIRDFDGQPFIMYSQTEARYFHDLLVSQFMRAEVLPRYVQHLSQIHSILAMVRAGLGL
AIVPAAAASLKIADVRLRPLKLRNPTPAELFLVWRREHDNPLLPALVELAGRLAVQQTED

Sequences:

>Translated_300_residues
MFDLNQLRCFVTVAEELHFGRAAVRLNMTQPPLSRQIQVLEHIIDAALLERTSRSVKLTPAGRSFLPEARRILKMAESAA
QVAQRIASGKSGSLKIGFTAAAAYGFLPDLVAACRSELPEADLSLKEMVSGDQIEALASGQIDAGLLRPPIARPEFATRR
VVAEPLCAAVPRRHRLASADAVSIRDFDGQPFIMYSQTEARYFHDLLVSQFMRAEVLPRYVQHLSQIHSILAMVRAGLGL
AIVPAAAASLKIADVRLRPLKLRNPTPAELFLVWRREHDNPLLPALVELAGRLAVQQTED
>Mature_300_residues
MFDLNQLRCFVTVAEELHFGRAAVRLNMTQPPLSRQIQVLEHIIDAALLERTSRSVKLTPAGRSFLPEARRILKMAESAA
QVAQRIASGKSGSLKIGFTAAAAYGFLPDLVAACRSELPEADLSLKEMVSGDQIEALASGQIDAGLLRPPIARPEFATRR
VVAEPLCAAVPRRHRLASADAVSIRDFDGQPFIMYSQTEARYFHDLLVSQFMRAEVLPRYVQHLSQIHSILAMVRAGLGL
AIVPAAAASLKIADVRLRPLKLRNPTPAELFLVWRREHDNPLLPALVELAGRLAVQQTED

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=279, Percent_Identity=38.3512544802867, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI2367136, Length=291, Percent_Identity=32.3024054982818, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788887, Length=282, Percent_Identity=31.5602836879433, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=28.1124497991968, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788748, Length=279, Percent_Identity=26.5232974910394, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=27.2357723577236, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=28.0487804878049, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790208, Length=299, Percent_Identity=27.7591973244147, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87081904, Length=155, Percent_Identity=32.258064516129, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=26.4, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=295, Percent_Identity=24.7457627118644, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=140, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=244, Percent_Identity=25.8196721311475, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=253, Percent_Identity=25.296442687747, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788626, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786713, Length=89, Percent_Identity=42.6966292134831, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=107, Percent_Identity=35.5140186915888, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33021; Mature: 33021

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLNQLRCFVTVAEELHFGRAAVRLNMTQPPLSRQIQVLEHIIDAALLERTSRSVKLTP
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
AGRSFLPEARRILKMAESAAQVAQRIASGKSGSLKIGFTAAAAYGFLPDLVAACRSELPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
ADLSLKEMVSGDQIEALASGQIDAGLLRPPIARPEFATRRVVAEPLCAAVPRRHRLASAD
CCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCC
AVSIRDFDGQPFIMYSQTEARYFHDLLVSQFMRAEVLPRYVQHLSQIHSILAMVRAGLGL
CEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AIVPAAAASLKIADVRLRPLKLRNPTPAELFLVWRREHDNPLLPALVELAGRLAVQQTED
EEHHHHHHHEEEEEEEEEEEECCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFDLNQLRCFVTVAEELHFGRAAVRLNMTQPPLSRQIQVLEHIIDAALLERTSRSVKLTP
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
AGRSFLPEARRILKMAESAAQVAQRIASGKSGSLKIGFTAAAAYGFLPDLVAACRSELPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
ADLSLKEMVSGDQIEALASGQIDAGLLRPPIARPEFATRRVVAEPLCAAVPRRHRLASAD
CCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCC
AVSIRDFDGQPFIMYSQTEARYFHDLLVSQFMRAEVLPRYVQHLSQIHSILAMVRAGLGL
CEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AIVPAAAASLKIADVRLRPLKLRNPTPAELFLVWRREHDNPLLPALVELAGRLAVQQTED
EEHHHHHHHEEEEEEEEEEEECCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]