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The map label for this gene is fucA [C]

Identifier: 146338632

GI number: 146338632

Start: 1670445

End: 1671113

Strand: Reverse

Name: fucA [C]

Synonym: BRADO1560

Alternate gene names: 146338632

Gene position: 1671113-1670445 (Counterclockwise)

Preceding gene: 146338633

Following gene: 146338631

Centisome position: 22.41

GC content: 65.32

Gene sequence:

>669_bases
GTGCTCGCGGATCTCGGCGTCGTCGACGCCTTCGGCCATGTCAGCGCGCGCAACCCAGCCAACCCGCACCACTTCCTGAT
GTCGCGGTCGATCGCACCGGCGCAGGTCACGGCCAATGACATCATGGAGTTCGACGAGAACGGCGAGCCGGTCGATGCGA
GGAACCGCACCGTGTTCCTCGAACGCTTCATCCATGCCGAGGTCTACAAGGCGCGACCGGACGTCAAGGCCGTGGTGCAC
ACGCATTCGCCCGGCGTCGTGCCCTTCAGCGTCAGCCGGACGCCGTTGCGGCCGCTCTACCACAACGCCGCCTTTCTCGG
CGACGGCGCGCCAGTGTGGGACATCGGCAAGGAATTCGGCGCCACCGACATGCTGGTGCGCAACAACGCGATCGGCAAAT
CGCTCGCATCTGCGCTCGGCGACAAATCTGTCATCCTGATGCGCGGCCATGGCGATGTCATCGTCGGCCCCACCGTGCAG
GCGGCCGTATTCCGCGCCTATTACACCGACGTGAATGCGAGGCTGCAGGCCCAGGCACTGGGCCTCGGTTCTGACGTGAC
CTATCTCAGCGCAGAGGAGGCGGCCAAGGCCGACGGCGTCAACATGGTGATCATGGACCGCGCCTGGGCGCTCTGGAAAC
AGAAGGTGCAGGCGCAGTCAGGCAAGTGA

Upstream 100 bases:

>100_bases
CATCACGGCCCTCCTTCTGTCGACAGGCCTTTCCCGCGCGCAAGACGCCATCCCCGGCGTCCCTCGCGAACTCCTCGAGG
ACATTGCCACTGGCAGCCGT

Downstream 100 bases:

>100_bases
TCGCCAGCCGGCTCCCACGATCGCGCGACCTGATTTTGCCGCAAAGTTGACACATGATTTGACTGAGTCGTTACCCCCTC
CATCGTCATTTCCCGGACCG

Product: putative aldolase

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLADLGVVDAFGHVSARNPANPHHFLMSRSIAPAQVTANDIMEFDENGEPVDARNRTVFLERFIHAEVYKARPDVKAVVH
THSPGVVPFSVSRTPLRPLYHNAAFLGDGAPVWDIGKEFGATDMLVRNNAIGKSLASALGDKSVILMRGHGDVIVGPTVQ
AAVFRAYYTDVNARLQAQALGLGSDVTYLSAEEAAKADGVNMVIMDRAWALWKQKVQAQSGK

Sequences:

>Translated_222_residues
MLADLGVVDAFGHVSARNPANPHHFLMSRSIAPAQVTANDIMEFDENGEPVDARNRTVFLERFIHAEVYKARPDVKAVVH
THSPGVVPFSVSRTPLRPLYHNAAFLGDGAPVWDIGKEFGATDMLVRNNAIGKSLASALGDKSVILMRGHGDVIVGPTVQ
AAVFRAYYTDVNARLQAQALGLGSDVTYLSAEEAAKADGVNMVIMDRAWALWKQKVQAQSGK
>Mature_222_residues
MLADLGVVDAFGHVSARNPANPHHFLMSRSIAPAQVTANDIMEFDENGEPVDARNRTVFLERFIHAEVYKARPDVKAVVH
THSPGVVPFSVSRTPLRPLYHNAAFLGDGAPVWDIGKEFGATDMLVRNNAIGKSLASALGDKSVILMRGHGDVIVGPTVQ
AAVFRAYYTDVNARLQAQALGLGSDVTYLSAEEAAKADGVNMVIMDRAWALWKQKVQAQSGK

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI89276787, Length=189, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI9257190, Length=189, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI297307160, Length=189, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI297307158, Length=189, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9257192, Length=189, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71989555, Length=195, Percent_Identity=29.7435897435897, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71989550, Length=195, Percent_Identity=29.7435897435897, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71989560, Length=197, Percent_Identity=29.4416243654822, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71989541, Length=197, Percent_Identity=29.4416243654822, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71989544, Length=195, Percent_Identity=29.7435897435897, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533218, Length=191, Percent_Identity=24.0837696335079, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI115533216, Length=191, Percent_Identity=24.0837696335079, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 24004; Mature: 24004

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADLGVVDAFGHVSARNPANPHHFLMSRSIAPAQVTANDIMEFDENGEPVDARNRTVFL
CCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCEECHHHHHHHCCCCCCCCCCCCCHHH
ERFIHAEVYKARPDVKAVVHTHSPGVVPFSVSRTPLRPLYHNAAFLGDGAPVWDIGKEFG
HHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCHHHCCEEEECCCCCHHHHHHHCC
ATDMLVRNNAIGKSLASALGDKSVILMRGHGDVIVGPTVQAAVFRAYYTDVNARLQAQAL
CCCEEEECCHHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHCCCCEEEEEEE
GLGSDVTYLSAEEAAKADGVNMVIMDRAWALWKQKVQAQSGK
CCCCCCEEEEHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLADLGVVDAFGHVSARNPANPHHFLMSRSIAPAQVTANDIMEFDENGEPVDARNRTVFL
CCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCEECHHHHHHHCCCCCCCCCCCCCHHH
ERFIHAEVYKARPDVKAVVHTHSPGVVPFSVSRTPLRPLYHNAAFLGDGAPVWDIGKEFG
HHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCHHHCCEEEECCCCCHHHHHHHCC
ATDMLVRNNAIGKSLASALGDKSVILMRGHGDVIVGPTVQAAVFRAYYTDVNARLQAQAL
CCCEEEECCHHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHCCCCEEEEEEE
GLGSDVTYLSAEEAAKADGVNMVIMDRAWALWKQKVQAQSGK
CCCCCCEEEEHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]