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The map label for this gene is prfA [H]

Identifier: 146337703

GI number: 146337703

Start: 587353

End: 588438

Strand: Reverse

Name: prfA [H]

Synonym: BRADO0572

Alternate gene names: 146337703

Gene position: 588438-587353 (Counterclockwise)

Preceding gene: 146337704

Following gene: 146337702

Centisome position: 7.89

GC content: 67.22

Gene sequence:

>1086_bases
ATGTCGTCGCTTCCCGAAGCCAAACTGGACGTCCTGCTCGCGCATCACGCCTCGCTGGAGGCGGAGCTGCTCGGCCAGGT
CAACTCCGACCGCTACGTGCAGATCACCCGCGAGCTCGCCGAGCTCAATCCGCTGATCGACGCCGTGAAGGCTTATCGTG
CGGGCGTCAAAGAACTCGCCGACACTGAGGCGCTGATCGCCGATCCCGACACCGATGCCGAGATGCGCGCGATGGCGGAG
AGCGAGCGCGACGAGCTGGTCGCGCGCCGCGGCGAGCTGGAGCAGAAGATCCGCATCGCGCTCCTGCCCAAGGACGCGAT
GGACGATCGCAACGTCGTGCTCGAAATCCGCGCCGGCACCGGCGGCGACGAGGCCTCTCTGTTCGCCGGCGATCTGTTCC
GGATGTACGAGCGCTTCGCGAGCTTGCAGGGCTGGAAGGTCGAGGTGATCTCGGCGTCAGAGGGCACCGTCGGCGGCTAC
AAGGAAATCATCGCCGAGGTGCAGGGCCGCGGCGCGTTCGCCAAGCTGAAATTCGAATCCGGCGTCCACCGCGTGCAGCG
CGTGCCCGACACCGAGACGCAGGGCCGCATCCACACCTCGGCGGCGACGGTGGCCGTGCTGCCGGAGATCGAGGACGTCG
ACGTCGACATCAAGCAGGACGATCTGCGCATCGAGACCATGCGGGCGCAAGGCGCGGGCGGCCAGCACGTCAACAAGACC
GAATCGGCGATCCGCATCACCCACATTCCGACCGGCATCGTGGTGATGATGCAGGACAGCCGCTCGCAGCATAAGAACCG
GGCGTCGGCGATGAACATTTTGCGCTCGCGCATCTACGACGCCGAGCGGCAGAAGGTCGACGCCGCGCGCTCCGCCGAGC
GCAAGGAGAAGGTCGGCTCCGGCGACCGCTCCGAACGCATCCGCACCTACAATTTCCCGCAAGGGCGCGTCACCGACCAC
CGAATCAACCTGACGCTCTACAAGCTGCCGCAGGTGATTGCCGGCGAAGCGCTTGGCGAGCTGGTCGACGCGCTCACCAC
GGAGCACCAGGCGGCGCAGCTCGCCGCGCAAGGCGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
TGACCGAGTTCGCCGAGAGCGAAGGCCTCGCGCTGTAGCGGGCCAGCCGCGGCCCGCGCGACTTCCGTTCGTATCGTTTG
CTCCTACATCAGATTACATC

Downstream 100 bases:

>100_bases
TGAAGGCGCGCGGATTGCGAGCGGCGGTGCGCCGCTGCCTCGACACAAGTGGTGTCGTCCCGGCGAACGCCGGGACCCAT
AACCACCGGCCTCAGTGGTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSSLPEAKLDVLLAHHASLEAELLGQVNSDRYVQITRELAELNPLIDAVKAYRAGVKELADTEALIADPDTDAEMRAMAE
SERDELVARRGELEQKIRIALLPKDAMDDRNVVLEIRAGTGGDEASLFAGDLFRMYERFASLQGWKVEVISASEGTVGGY
KEIIAEVQGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEIEDVDVDIKQDDLRIETMRAQGAGGQHVNKT
ESAIRITHIPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAERQKVDAARSAERKEKVGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLPQVIAGEALGELVDALTTEHQAAQLAAQGAAA

Sequences:

>Translated_361_residues
MSSLPEAKLDVLLAHHASLEAELLGQVNSDRYVQITRELAELNPLIDAVKAYRAGVKELADTEALIADPDTDAEMRAMAE
SERDELVARRGELEQKIRIALLPKDAMDDRNVVLEIRAGTGGDEASLFAGDLFRMYERFASLQGWKVEVISASEGTVGGY
KEIIAEVQGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEIEDVDVDIKQDDLRIETMRAQGAGGQHVNKT
ESAIRITHIPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAERQKVDAARSAERKEKVGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLPQVIAGEALGELVDALTTEHQAAQLAAQGAAA
>Mature_360_residues
SSLPEAKLDVLLAHHASLEAELLGQVNSDRYVQITRELAELNPLIDAVKAYRAGVKELADTEALIADPDTDAEMRAMAES
ERDELVARRGELEQKIRIALLPKDAMDDRNVVLEIRAGTGGDEASLFAGDLFRMYERFASLQGWKVEVISASEGTVGGYK
EIIAEVQGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEIEDVDVDIKQDDLRIETMRAQGAGGQHVNKTE
SAIRITHIPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAERQKVDAARSAERKEKVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLPQVIAGEALGELVDALTTEHQAAQLAAQGAAA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=338, Percent_Identity=41.7159763313609, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI34577120, Length=327, Percent_Identity=37.0030581039755, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI166795305, Length=243, Percent_Identity=39.0946502057613, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=54.3661971830986, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=313, Percent_Identity=36.741214057508, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=301, Percent_Identity=38.5382059800665, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6321295, Length=323, Percent_Identity=43.6532507739938, Blast_Score=263, Evalue=4e-71,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=41.9939577039275, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39684; Mature: 39553

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1 ; PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPEAKLDVLLAHHASLEAELLGQVNSDRYVQITRELAELNPLIDAVKAYRAGVKELA
CCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTEALIADPDTDAEMRAMAESERDELVARRGELEQKIRIALLPKDAMDDRNVVLEIRAGT
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCC
GGDEASLFAGDLFRMYERFASLQGWKVEVISASEGTVGGYKEIIAEVQGRGAFAKLKFES
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHH
GVHRVQRVPDTETQGRIHTSAATVAVLPEIEDVDVDIKQDDLRIETMRAQGAGGQHVNKT
HHHHHHCCCCCCCCCEEEECCEEEEECCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCC
ESAIRITHIPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAERQKVDAARSAERKEKVGS
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLPQVIAGEALGELVDALTTEHQAAQLAAQGAA
CCCCCCCEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
A
C
>Mature Secondary Structure 
SSLPEAKLDVLLAHHASLEAELLGQVNSDRYVQITRELAELNPLIDAVKAYRAGVKELA
CCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTEALIADPDTDAEMRAMAESERDELVARRGELEQKIRIALLPKDAMDDRNVVLEIRAGT
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCC
GGDEASLFAGDLFRMYERFASLQGWKVEVISASEGTVGGYKEIIAEVQGRGAFAKLKFES
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHH
GVHRVQRVPDTETQGRIHTSAATVAVLPEIEDVDVDIKQDDLRIETMRAQGAGGQHVNKT
HHHHHHCCCCCCCCCEEEECCEEEEECCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCC
ESAIRITHIPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAERQKVDAARSAERKEKVGS
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLPQVIAGEALGELVDALTTEHQAAQLAAQGAA
CCCCCCCEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]