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The map label for this gene is hemK [H]

Identifier: 146337702

GI number: 146337702

Start: 586272

End: 587159

Strand: Reverse

Name: hemK [H]

Synonym: BRADO0571

Alternate gene names: 146337702

Gene position: 587159-586272 (Counterclockwise)

Preceding gene: 146337703

Following gene: 146337701

Centisome position: 7.87

GC content: 70.5

Gene sequence:

>888_bases
ATGACCAAGATCGCCGCCGGCGCCAGCATCGATTCGGCGCGACGCGCGCTCGCGGCGCGCTTCGGCGACGCCGGTTTGGA
CTCGCCCGAGCTCGACGCGCGGCTTCTGGTGGGCCATGCGCTGCAGCTCGATCTGACCGGTCTGGTGATGCACGGCAAGC
GCGCCCTCTCCCATGACGATGCGCAACGACTCGACGCACTGGTGCAGCGCCGCCTCGCTGGCGAACCGGTGGCCCGCATC
CTCGGGCACAAGGAGTTCTGGGGACTCGAGCTCCGGCTGTCGGCTGACACGCTGGTGCCCCGGCCGGACACCGAGACCGT
CGTCGAGCTGGCGCTGGACCACCTCTCCGCCGGCGGCGCGCTGACGCGGCCGCTGCGCATCGGCGATCTCGGCACCGGAT
CGGGCGCCATCCTCCTGGCCCTGCTGTCCGAGCTGCCGCGGGCCTCCGGCGTCGGGACCGACATCAGCATCGCCGCGCTG
ACGACGGCACGCGAGAATGCGCGTGCGCTCGGTCTGAACGACCGCGCCGCTTTCGTCGCCTGCTCCTATGCGACCGCGCT
CGCGCCGCCGTTCGATCTGATCGTGTCGAATCCGCCCTACATTCCCTCGGCCGACATCGCCGGTCTCGCGACCGAGGTGC
ACGCTCATGACCCGCTCCGGGCACTTGATGGCGGCGGTGACGGTCTCGCCGCCTATCGCACACTGATCCCACAGGCCGCG
CTGCTGCTTCAGACCGGCGGCGCCGTCATCGTCGAGGTCGGTCAGGGCCAGAGCGGCGACGTCGCGGGGCTGATGACCGA
GGCCGGTCTGGCGAGCGATTCGTCGGCCATCAGGGCCGATCTCGCCGGTATTCCGAGGGCCGTCATGGCCCTAAAAAAGC
GCCGGTAA

Upstream 100 bases:

>100_bases
AGTGGTTGTCACGAAAGGCAACTCCGACCACCCGTCCCGCGCTTGCACGGCGTATGGATCCCCGCTTTCGCGGGGATGAC
GCGTGGAGAAAGCGTGCGCG

Downstream 100 bases:

>100_bases
AGGCTGGCAGACGCAGAAAAAGCTCTTGGAATATCCTCGAGGAGCGACTACGTTTCGTCCATAGCATCGGCGCAGGCCCC
GCTGAGCGGTATTCACGGAA

Product: putative methyltransferase hemK modifies release factors RF-1 and RF-2

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTKIAAGASIDSARRALAARFGDAGLDSPELDARLLVGHALQLDLTGLVMHGKRALSHDDAQRLDALVQRRLAGEPVARI
LGHKEFWGLELRLSADTLVPRPDTETVVELALDHLSAGGALTRPLRIGDLGTGSGAILLALLSELPRASGVGTDISIAAL
TTARENARALGLNDRAAFVACSYATALAPPFDLIVSNPPYIPSADIAGLATEVHAHDPLRALDGGGDGLAAYRTLIPQAA
LLLQTGGAVIVEVGQGQSGDVAGLMTEAGLASDSSAIRADLAGIPRAVMALKKRR

Sequences:

>Translated_295_residues
MTKIAAGASIDSARRALAARFGDAGLDSPELDARLLVGHALQLDLTGLVMHGKRALSHDDAQRLDALVQRRLAGEPVARI
LGHKEFWGLELRLSADTLVPRPDTETVVELALDHLSAGGALTRPLRIGDLGTGSGAILLALLSELPRASGVGTDISIAAL
TTARENARALGLNDRAAFVACSYATALAPPFDLIVSNPPYIPSADIAGLATEVHAHDPLRALDGGGDGLAAYRTLIPQAA
LLLQTGGAVIVEVGQGQSGDVAGLMTEAGLASDSSAIRADLAGIPRAVMALKKRR
>Mature_294_residues
TKIAAGASIDSARRALAARFGDAGLDSPELDARLLVGHALQLDLTGLVMHGKRALSHDDAQRLDALVQRRLAGEPVARIL
GHKEFWGLELRLSADTLVPRPDTETVVELALDHLSAGGALTRPLRIGDLGTGSGAILLALLSELPRASGVGTDISIAALT
TARENARALGLNDRAAFVACSYATALAPPFDLIVSNPPYIPSADIAGLATEVHAHDPLRALDGGGDGLAAYRTLIPQAAL
LLQTGGAVIVEVGQGQSGDVAGLMTEAGLASDSSAIRADLAGIPRAVMALKKRR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=244, Percent_Identity=35.655737704918, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1787463, Length=231, Percent_Identity=38.0952380952381, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082085, Length=256, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582226, Length=252, Percent_Identity=28.968253968254, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30421; Mature: 30290

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAAGASIDSARRALAARFGDAGLDSPELDARLLVGHALQLDLTGLVMHGKRALSHDD
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEEHHHHEECCCHHCCCCH
AQRLDALVQRRLAGEPVARILGHKEFWGLELRLSADTLVPRPDTETVVELALDHLSAGGA
HHHHHHHHHHHHCCCHHHHHHCCCHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
LTRPLRIGDLGTGSGAILLALLSELPRASGVGTDISIAALTTARENARALGLNDRAAFVA
CCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCEEECCCCCHHEEE
CSYATALAPPFDLIVSNPPYIPSADIAGLATEVHAHDPLRALDGGGDGLAAYRTLIPQAA
EHHHHHHCCCHHEEECCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHH
LLLQTGGAVIVEVGQGQSGDVAGLMTEAGLASDSSAIRADLAGIPRAVMALKKRR
HHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
TKIAAGASIDSARRALAARFGDAGLDSPELDARLLVGHALQLDLTGLVMHGKRALSHDD
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEEHHHHEECCCHHCCCCH
AQRLDALVQRRLAGEPVARILGHKEFWGLELRLSADTLVPRPDTETVVELALDHLSAGGA
HHHHHHHHHHHHCCCHHHHHHCCCHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
LTRPLRIGDLGTGSGAILLALLSELPRASGVGTDISIAALTTARENARALGLNDRAAFVA
CCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCEEECCCCCHHEEE
CSYATALAPPFDLIVSNPPYIPSADIAGLATEVHAHDPLRALDGGGDGLAAYRTLIPQAA
EHHHHHHCCCHHEEECCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHH
LLLQTGGAVIVEVGQGQSGDVAGLMTEAGLASDSSAIRADLAGIPRAVMALKKRR
HHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]