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The map label for this gene is mdh [H]

Identifier: 146337549

GI number: 146337549

Start: 409671

End: 410639

Strand: Direct

Name: mdh [H]

Synonym: BRADO0403

Alternate gene names: 146337549

Gene position: 409671-410639 (Clockwise)

Preceding gene: 146337548

Following gene: 146337550

Centisome position: 5.49

GC content: 66.15

Gene sequence:

>969_bases
ATGGCGCGCGACAAGATTGCTTTGATTGGCTCCGGTCAGATCGGCGGAACGCTGGCTCACCTCGTCGGCCTCAAGGAGCT
CGGCGACGTCGTGATGTTCGATATTGCCGAAGGCGTTCCGCAGGGCAAGGCGCTCGACATCGCGCAGTCCTCGCCGGTCG
ACGGCTTCGACGCCCACTACACCGGCGCCAACTCCTACGAGGCGCTCGACAACGCCAAGGTGTGCATCGTCACCGCCGGC
GTGCCGCGCAAGCCGGGCATGAGCCGCGATGACCTGCTGTCGATCAACCTCAAGGTCATGGAGCAGGTCGGCGCCGGCAT
CAAGAAGTACGCCCCCGACGCGTTCGTCATCTGCATCACCAACCCGCTCGACGCGATGGTGTGGGCGCTGCAGAAGGCCT
CGGGCCTTCCCGCAAAGAAGGTCGTCGGCATGGCCGGCGTGCTCGACAGCGCGCGCTTCCGCTACTTCCTGGCCGATGAG
TTCAACGTCTCGGTCGAGGACGTCACCGCCTTCGTGCTCGGCGGCCATGGCGACACCATGGTGCCGCTGACCAAGTACTC
CACCGTTGCCGGCATCCCGCTGCCGGACCTCGTCAAGATGGGCTGGACCTCGCAGGCGCGCATCGACGAGATCGTCGACC
GCACCCGCAACGGCGGTGCCGAGATCGTCAACCTGCTCAAGACCGGCTCGGCCTACTACGCCCCGGCCGCCTCGGCGATC
GCGATGGCCGAAAGCTATCTGCGCGACAAGAAGCGCGTGCTGCCCTGCGCCGCCTATCTCAACGGCGAGTTCGGCGTGAA
GGACATGTATGTCGGCGTGCCCGTCGTCATCGGTTCGAAGGGTGTCGAGCGTATCGTCGAGATCGAGCTGGCCGGCAAGG
ATCGCGAGGCCTTCGACAAGTCGGTCGCCGCCGTGCAGGGCCTGGTCGACGCCTGCAAGAAGATCGCGCCCGACCTGCTC
GGCCGCTGA

Upstream 100 bases:

>100_bases
ATTGGCCATAGCGTGACTTGAACGGCCCGGGCGAAAGGGATAACCACCCACTCGACTTTTCAACCTCCCTCACCCCTTCG
GGTTCAAAGGAAAATTTGCC

Downstream 100 bases:

>100_bases
GGGTAGCAGGGCGGGCGGCCGGCCCGCTCCGAAGAGTTGCAGGATGCTTGGTATATGGTATACCAAGCATCCGCAAAACA
CTTATAGACGTCACCACCCC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MARDKIALIGSGQIGGTLAHLVGLKELGDVVMFDIAEGVPQGKALDIAQSSPVDGFDAHYTGANSYEALDNAKVCIVTAG
VPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADE
FNVSVEDVTAFVLGGHGDTMVPLTKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAYYAPAASAI
AMAESYLRDKKRVLPCAAYLNGEFGVKDMYVGVPVVIGSKGVERIVEIELAGKDREAFDKSVAAVQGLVDACKKIAPDLL
GR

Sequences:

>Translated_322_residues
MARDKIALIGSGQIGGTLAHLVGLKELGDVVMFDIAEGVPQGKALDIAQSSPVDGFDAHYTGANSYEALDNAKVCIVTAG
VPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADE
FNVSVEDVTAFVLGGHGDTMVPLTKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAYYAPAASAI
AMAESYLRDKKRVLPCAAYLNGEFGVKDMYVGVPVVIGSKGVERIVEIELAGKDREAFDKSVAAVQGLVDACKKIAPDLL
GR
>Mature_321_residues
ARDKIALIGSGQIGGTLAHLVGLKELGDVVMFDIAEGVPQGKALDIAQSSPVDGFDAHYTGANSYEALDNAKVCIVTAGV
PRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADEF
NVSVEDVTAFVLGGHGDTMVPLTKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAYYAPAASAIA
MAESYLRDKKRVLPCAAYLNGEFGVKDMYVGVPVVIGSKGVERIVEIELAGKDREAFDKSVAAVQGLVDACKKIAPDLLG
R

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=28.8524590163934, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=28.8524590163934, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=29.8701298701299, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=29.8701298701299, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=28.525641025641, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=28.525641025641, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=25.1572327044025, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI291575128, Length=321, Percent_Identity=28.0373831775701, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4557032, Length=321, Percent_Identity=28.0373831775701, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=31.5, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=31.5, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=28.7179487179487, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI21735621, Length=292, Percent_Identity=26.7123287671233, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI103472011, Length=319, Percent_Identity=20.3761755485893, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789632, Length=325, Percent_Identity=30.1538461538462, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=285, Percent_Identity=26.3157894736842, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=309, Percent_Identity=29.7734627831715, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=28.0936454849498, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=243, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324446, Length=254, Percent_Identity=27.5590551181102, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=28.0528052805281, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI45550422, Length=299, Percent_Identity=25.4180602006689, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24647881, Length=292, Percent_Identity=29.1095890410959, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24663595, Length=286, Percent_Identity=29.3706293706294, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=26.6233766233766, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34092; Mature: 33961

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDKIALIGSGQIGGTLAHLVGLKELGDVVMFDIAEGVPQGKALDIAQSSPVDGFDAHY
CCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEHHHHCCCCCCCEEECCCCCCCCCCCCCC
TGANSYEALDNAKVCIVTAGVPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICIT
CCCCCHHHCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADEFNVSVEDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEECCCCCCE
VPLTKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAYYAPAASAI
EEEECCCEECCCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
AMAESYLRDKKRVLPCAAYLNGEFGVKDMYVGVPVVIGSKGVERIVEIELAGKDREAFDK
HHHHHHHHHHHHHCHHHEEECCCCCCEEEEECCEEEECCCCCCEEEEEEECCCCHHHHHH
SVAAVQGLVDACKKIAPDLLGR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARDKIALIGSGQIGGTLAHLVGLKELGDVVMFDIAEGVPQGKALDIAQSSPVDGFDAHY
CCCCEEEEECCCCCHHHHHHHHHHHHHHEEEHHHHCCCCCCCEEECCCCCCCCCCCCCC
TGANSYEALDNAKVCIVTAGVPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICIT
CCCCCHHHCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKASGLPAKKVVGMAGVLDSARFRYFLADEFNVSVEDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEECCCCCCE
VPLTKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAYYAPAASAI
EEEECCCEECCCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
AMAESYLRDKKRVLPCAAYLNGEFGVKDMYVGVPVVIGSKGVERIVEIELAGKDREAFDK
HHHHHHHHHHHHHCHHHEEECCCCCCEEEEECCEEEECCCCCCEEEEEEECCCCHHHHHH
SVAAVQGLVDACKKIAPDLLGR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA