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The map label for this gene is ligT [C]

Identifier: 146337547

GI number: 146337547

Start: 407568

End: 408107

Strand: Direct

Name: ligT [C]

Synonym: BRADO0401

Alternate gene names: 146337547

Gene position: 407568-408107 (Clockwise)

Preceding gene: 146337546

Following gene: 146337548

Centisome position: 5.47

GC content: 66.3

Gene sequence:

>540_bases
ATGCCGCGTCTGTTTACTGGACTGGAGATCCCGGCCGAGGTCGGCCAGACCCTCTCCACTCTGCGGGGTGGCCTTCCCGG
CGCCCGCTGGATCGATCCTGAAAATTATCACGTCACCTTGCGCTTCATCGGCGACATCGACGGCGCGTCCGCCAACGAGA
TCGCGGCGATGCTGTGGCGGGTCAACCGCAAGCCGTTCGAGGTCACGGTGCAGGGCCTCTCCAGCTTCGGCGGCCGCAAG
CCGCGGGCGGTTGTCGCCAACATCGCGCCCAGCAGGCCGCTGATCGAGCTGCAGGCCGAGCTGGAGCGGATGATGCAGCG
GATCGGGCTCGATCCGGAGGGCCGCAAATTCGTGCCCCATGTCACCCTGGCGCGGCTGCACGATGCGTCGAGTCAGGATG
TCGCCGATTATCTCTCGGTCCGCGGCTACTTCCCGAGCCGGGTTTTCATGGCCGAGCGCTTCGTGCTGTTTTCGTCCCGT
GCTTCCACGGGAGGCGGGCCCTATGTGGTGGAGGACGCCTACGATCTGTGCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGTAGGAATGTTGCATTGACGATTCGTCGTCGATCATTTCGTCAAGGCATGATCTCGTCAATTTTCGAGATCAGCCTCCA
GCATCGGGAGTCCTGAAACG

Downstream 100 bases:

>100_bases
GCCGGGGCCGACCGAGCAAGACGTGCTTAGCCCGCAACTAACGCGCGCCGGCGTGTTTGCGTGCCATATACCACGATCCC
ACGGTCCCGCTGCATGGCGG

Product: putative 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPRLFTGLEIPAEVGQTLSTLRGGLPGARWIDPENYHVTLRFIGDIDGASANEIAAMLWRVNRKPFEVTVQGLSSFGGRK
PRAVVANIAPSRPLIELQAELERMMQRIGLDPEGRKFVPHVTLARLHDASSQDVADYLSVRGYFPSRVFMAERFVLFSSR
ASTGGGPYVVEDAYDLCAA

Sequences:

>Translated_179_residues
MPRLFTGLEIPAEVGQTLSTLRGGLPGARWIDPENYHVTLRFIGDIDGASANEIAAMLWRVNRKPFEVTVQGLSSFGGRK
PRAVVANIAPSRPLIELQAELERMMQRIGLDPEGRKFVPHVTLARLHDASSQDVADYLSVRGYFPSRVFMAERFVLFSSR
ASTGGGPYVVEDAYDLCAA
>Mature_178_residues
PRLFTGLEIPAEVGQTLSTLRGGLPGARWIDPENYHVTLRFIGDIDGASANEIAAMLWRVNRKPFEVTVQGLSSFGGRKP
RAVVANIAPSRPLIELQAELERMMQRIGLDPEGRKFVPHVTLARLHDASSQDVADYLSVRGYFPSRVFMAERFVLFSSRA
STGGGPYVVEDAYDLCAA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19721; Mature: 19590

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTGLEIPAEVGQTLSTLRGGLPGARWIDPENYHVTLRFIGDIDGASANEIAAMLWR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
VNRKPFEVTVQGLSSFGGRKPRAVVANIAPSRPLIELQAELERMMQRIGLDPEGRKFVPH
CCCCCCEEEHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCH
VTLARLHDASSQDVADYLSVRGYFPSRVFMAERFVLFSSRASTGGGPYVVEDAYDLCAA
HHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCEEECCHHHHHCC
>Mature Secondary Structure 
PRLFTGLEIPAEVGQTLSTLRGGLPGARWIDPENYHVTLRFIGDIDGASANEIAAMLWR
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
VNRKPFEVTVQGLSSFGGRKPRAVVANIAPSRPLIELQAELERMMQRIGLDPEGRKFVPH
CCCCCCEEEHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCH
VTLARLHDASSQDVADYLSVRGYFPSRVFMAERFVLFSSRASTGGGPYVVEDAYDLCAA
HHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]