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The map label for this gene is tesA [H]

Identifier: 146337546

GI number: 146337546

Start: 406729

End: 407361

Strand: Direct

Name: tesA [H]

Synonym: BRADO0400

Alternate gene names: 146337546

Gene position: 406729-407361 (Clockwise)

Preceding gene: 146337541

Following gene: 146337547

Centisome position: 5.45

GC content: 65.4

Gene sequence:

>633_bases
GTGCACATACTCGTGTTGATGCTCGCCTTGATGACAGCCGGACCGGCGTTCGCGGGCACTGAGGCCGGCAAGCCGATCAA
GCTGGTGGTGCTCGGCGATTCGCTGAGCGCCGGCCTCGGCCTGTCCGCCTCCGACTCTTTCCCCTCCAAACTGCAAAAGG
CGTTGCAGGCCAAGGGGCTGGCGATCGACTTGACCAATGCCGGGGTGTCCGGAGACACCTCCTCCGGCGGCCGCGACCGG
CTCGACTGGTCGGTGGCCGACGGGACCGAGGCTGTCATCGTCGAGCTCGGCGCCAATGATGCTCTGCGCGGCATCGATCC
GTCCGTCACCCGGCAGGCCCTGACCGAGATCGTCAAGCGGCTGAAGGCGCGTGGCATCGCCGTCATGCTGTGCGGCATGC
TCGCGCCGCCGAATTACGGCAGCGACTACGCTGTTAAGTTCAATGCGATCTACCCTGACCTCGCCAAGGAGTTCGACGTC
CCGCTCTACCCGTTCTTCCTCGACGGCGTTGCTGCCGACCCCAAGCTCAACCAGGCGGACGGCATTCACCCGACCGCCGC
CGGCGTCGATATCATCGTCTCCCGCATGCTGCCTACGGTCGAAGCATTCCTGCGTCCGCTTCGTGGGCAATGA

Upstream 100 bases:

>100_bases
CGGTGCTGTGCCGGCCAAACGTGAGGTTTCGATGAGACTGTCCATGGCTCGGTCATATGGCAACTCCGCAAGCCGGGTCG
AGGGGCGCGGAGGCATCTTC

Downstream 100 bases:

>100_bases
AGGAACGAAGCCCGCCTCCGGACATTTTGCGATAACCTTACGGACCGTGTTTTCCGCAGGTTTTCACCGCGTTAACGCTC
GCTCCGTCGCGGAGTCATAT

Product: multifunctional acyl-CoA thioesterase I/protease I/lysophospholipasel(I)

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MHILVLMLALMTAGPAFAGTEAGKPIKLVVLGDSLSAGLGLSASDSFPSKLQKALQAKGLAIDLTNAGVSGDTSSGGRDR
LDWSVADGTEAVIVELGANDALRGIDPSVTRQALTEIVKRLKARGIAVMLCGMLAPPNYGSDYAVKFNAIYPDLAKEFDV
PLYPFFLDGVAADPKLNQADGIHPTAAGVDIIVSRMLPTVEAFLRPLRGQ

Sequences:

>Translated_210_residues
MHILVLMLALMTAGPAFAGTEAGKPIKLVVLGDSLSAGLGLSASDSFPSKLQKALQAKGLAIDLTNAGVSGDTSSGGRDR
LDWSVADGTEAVIVELGANDALRGIDPSVTRQALTEIVKRLKARGIAVMLCGMLAPPNYGSDYAVKFNAIYPDLAKEFDV
PLYPFFLDGVAADPKLNQADGIHPTAAGVDIIVSRMLPTVEAFLRPLRGQ
>Mature_210_residues
MHILVLMLALMTAGPAFAGTEAGKPIKLVVLGDSLSAGLGLSASDSFPSKLQKALQAKGLAIDLTNAGVSGDTSSGGRDR
LDWSVADGTEAVIVELGANDALRGIDPSVTRQALTEIVKRLKARGIAVMLCGMLAPPNYGSDYAVKFNAIYPDLAKEFDV
PLYPFFLDGVAADPKLNQADGIHPTAAGVDIIVSRMLPTVEAFLRPLRGQ

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=197, Percent_Identity=42.6395939086294, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21946; Mature: 21946

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILVLMLALMTAGPAFAGTEAGKPIKLVVLGDSLSAGLGLSASDSFPSKLQKALQAKGL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCC
AIDLTNAGVSGDTSSGGRDRLDWSVADGTEAVIVELGANDALRGIDPSVTRQALTEIVKR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LKARGIAVMLCGMLAPPNYGSDYAVKFNAIYPDLAKEFDVPLYPFFLDGVAADPKLNQAD
HHHCCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCC
GIHPTAAGVDIIVSRMLPTVEAFLRPLRGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHILVLMLALMTAGPAFAGTEAGKPIKLVVLGDSLSAGLGLSASDSFPSKLQKALQAKGL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCC
AIDLTNAGVSGDTSSGGRDRLDWSVADGTEAVIVELGANDALRGIDPSVTRQALTEIVKR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LKARGIAVMLCGMLAPPNYGSDYAVKFNAIYPDLAKEFDVPLYPFFLDGVAADPKLNQAD
HHHCCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCC
GIHPTAAGVDIIVSRMLPTVEAFLRPLRGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]