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The map label for this gene is pat [H]

Identifier: 146337542

GI number: 146337542

Start: 401410

End: 401937

Strand: Reverse

Name: pat [H]

Synonym: BRADO0396

Alternate gene names: 146337542

Gene position: 401937-401410 (Counterclockwise)

Preceding gene: 146337543

Following gene: 146337538

Centisome position: 5.39

GC content: 66.29

Gene sequence:

>528_bases
ATGTCCAGCATCGACATTCGCCCGACCACCGAAGCCGACCTTTCCGCCATCACCGCGATCTACGGCCAAGCCGTCCGCGA
GGGCACCGCCACGTTCGAGCTGGACCCTCCTGATCTGGCCGAGATGACCCGCCGCTTCCGGGCGCTGACTGACGGCGACT
TTCCCTATTTCGCCGCCGTGCTGGATGGGCAGGTGGTGGGCTATGCCTATGCCGGGCCGTACCGGCCGCGCCCCGCCTAT
CGCTTCACGGTCGAGAATTCCATCTATCTGGCGCCGGCAAGCCAGCGCCGCGGCATCGGCATGCGGCTCATGCGGCAGCT
GATCGCCGCGTGCGAGGAGAAGGGCTTCCGCCAGATGATCGCCGTGATCGGCGACTCCGCCAATGCCGGCTCGGTCGGGC
TGCATACCGCCTGCGGCTTCCAGATGATTGGCACCCATCCGAGCGTTGGCCTGAAGTTCGGGCGCTGGCTGGATACGGTC
ATGATGCAGCTGTCACTCGGCGCCGGAAGCAGCGACATTCCCGCTTGA

Upstream 100 bases:

>100_bases
GACGAGCGCACCGCTCCACCTGGGCGGCATCTCTGCACGACAAAACGGAGCCCTGTTCGGCGGAGGTTGAACAGCTTTCG
CGCGCTGGTATGTTCCGGAC

Downstream 100 bases:

>100_bases
GCGCGTCGCTCGCGCAGGGACCGTCCCGCTCAAACCAGCGCCGGCTTCGGCGCGATCGCCAGCAGCACCCGCTCAAGCTC
ATGGCCGCGGCGCAGGATCA

Product: phosphinothricin acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSSIDIRPTTEADLSAITAIYGQAVREGTATFELDPPDLAEMTRRFRALTDGDFPYFAAVLDGQVVGYAYAGPYRPRPAY
RFTVENSIYLAPASQRRGIGMRLMRQLIAACEEKGFRQMIAVIGDSANAGSVGLHTACGFQMIGTHPSVGLKFGRWLDTV
MMQLSLGAGSSDIPA

Sequences:

>Translated_175_residues
MSSIDIRPTTEADLSAITAIYGQAVREGTATFELDPPDLAEMTRRFRALTDGDFPYFAAVLDGQVVGYAYAGPYRPRPAY
RFTVENSIYLAPASQRRGIGMRLMRQLIAACEEKGFRQMIAVIGDSANAGSVGLHTACGFQMIGTHPSVGLKFGRWLDTV
MMQLSLGAGSSDIPA
>Mature_174_residues
SSIDIRPTTEADLSAITAIYGQAVREGTATFELDPPDLAEMTRRFRALTDGDFPYFAAVLDGQVVGYAYAGPYRPRPAYR
FTVENSIYLAPASQRRGIGMRLMRQLIAACEEKGFRQMIAVIGDSANAGSVGLHTACGFQMIGTHPSVGLKFGRWLDTVM
MQLSLGAGSSDIPA

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=163, Percent_Identity=37.4233128834356, Blast_Score=106, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 18917; Mature: 18786

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIDIRPTTEADLSAITAIYGQAVREGTATFELDPPDLAEMTRRFRALTDGDFPYFAAV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCHHEEEE
LDGQVVGYAYAGPYRPRPAYRFTVENSIYLAPASQRRGIGMRLMRQLIAACEEKGFRQMI
CCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVIGDSANAGSVGLHTACGFQMIGTHPSVGLKFGRWLDTVMMQLSLGAGSSDIPA
HHHCCCCCCCCCCEEHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSIDIRPTTEADLSAITAIYGQAVREGTATFELDPPDLAEMTRRFRALTDGDFPYFAAV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCHHEEEE
LDGQVVGYAYAGPYRPRPAYRFTVENSIYLAPASQRRGIGMRLMRQLIAACEEKGFRQMI
CCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVIGDSANAGSVGLHTACGFQMIGTHPSVGLKFGRWLDTVMMQLSLGAGSSDIPA
HHHCCCCCCCCCCEEHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA