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The map label for this gene is pncB [H]

Identifier: 146337478

GI number: 146337478

Start: 333731

End: 335035

Strand: Reverse

Name: pncB [H]

Synonym: BRADO0323

Alternate gene names: 146337478

Gene position: 335035-333731 (Counterclockwise)

Preceding gene: 146337479

Following gene: 146337477

Centisome position: 4.49

GC content: 64.75

Gene sequence:

>1305_bases
ATGACCGTTACAGACATCGCGAGTAGAACCTACAATCACGGCTGGCGCCTTGATCCGATCGTGCGCAGCCTGCTCGATAC
GGATTTCTACAAGCTTCTGATGCTGCAGATGATTCGGGAGTTCTACCCGGATCAGAAGGTGACCTTCTCGGTCATCAATC
GCACCAAGCGCGTGCGGCTCGGCGATGTCATCGACGAGGGCGAGCTGCGGGCCCAGCTCGACCACGCCCGCACCATCCGC
TTCTCCAAGAAGGAGCTGATCTGGCTGGCCGGTAACACGTTCTACGGCAAGACCCACATGTTCTCGCCGGACTTCCTGGC
CTGGCTCGCCAATTTCCGCCTGCCCGAATACGAGCTGCACAAGGCCGACGGCCAGTACGAGCTGCATTTCCACGGCCTGT
GGACCCACACCACGATGTGGGAAATTCCGGCGCTCGCGATCGTCAATGAGCTGCGCTCGCGGCAGGCCATGAAGGGTCAG
GGCCGCTTCGCGCTCGACGTGCTGTTCGCCCGCGCCAAGGCCAAGCTGTGGGCCAAGGTCGAGCGGCTGCGCAAGCTCGA
GGGGCTGCGGCTGTCGGATTTCGGCACCCGCCGCCGCCACGGCTTCCTGTGGCAGCGCTGGTGCGTCGAAGCCGTCAAGG
AAGGCCTGGGCTCCTCCTTCACCGGCACCTCGAACGTGCTGCTGGCGATGGATAATGATCTCGAAGCGATCGGCACCAAT
GCGCACGAGCTGCCCATGGTCGCGGCGGCGCTGGCCAAGGACGACGATGAACTGCGTTTCGCGCCCTACCGCATTCTCGA
CCAGTGGCGGCAGACCTATGCCGGCAATCTGCTGATCGCCCTGCCGGACGCGTTCGGCACCAAGGCTTTCCTGCGCGACG
CGCCGGACTGGGTCGCCGACTGGACCGGCTTCCGCCCCGACAGCGCGCCGCCGATTCAGGCGGGCGAGGAGATCATCGCG
TGGTGGAAGTCGAAGGGCCGTGATCCCCGACAGAAGCTCCTGGTGTTCTCCGACGCGATGGATGTCGAATCGATCGAGCA
GATCCACCAGCGCTTCTCCGACCAGGTCCGTCTGTCGTTCGGCTGGGGCACCAACCTGACCAACGACTTCGTCGGCTGCT
CGCCCGACGGTTCTGTCGATCTCGATCCGATTTCGCTGGTCTGCAAGGTGACCTCGGTCGACGGCCGCCCCGCCGTGAAA
CTGTCCGACAATCCGGAGAAGGCGACCGGCGACCCCGCCGAGATCGCCCGCTACCTGCGTGTCTTCGGCGACGCCGGCCG
CGTGCGGACCCCCGTGTTCGTCTGA

Upstream 100 bases:

>100_bases
CGATGAACCGCTTGATGCCGGGGACCTCTCCGGTCACCGCAACAGAACCCTGCGCCAAAGCCCAGTGGCGGGTTTCCGGC
CGGCGCTGTAAAACCGTCGC

Downstream 100 bases:

>100_bases
GCAGAGAAAAATCTCTCGCATCCTCACTGATTTTCGTTCCGGATTTGTTCGGCTTGCGGTAAAATCGGAGCCCGAACGGG
GCAACCGGAAGGCAGGAGAT

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTVTDIASRTYNHGWRLDPIVRSLLDTDFYKLLMLQMIREFYPDQKVTFSVINRTKRVRLGDVIDEGELRAQLDHARTIR
FSKKELIWLAGNTFYGKTHMFSPDFLAWLANFRLPEYELHKADGQYELHFHGLWTHTTMWEIPALAIVNELRSRQAMKGQ
GRFALDVLFARAKAKLWAKVERLRKLEGLRLSDFGTRRRHGFLWQRWCVEAVKEGLGSSFTGTSNVLLAMDNDLEAIGTN
AHELPMVAAALAKDDDELRFAPYRILDQWRQTYAGNLLIALPDAFGTKAFLRDAPDWVADWTGFRPDSAPPIQAGEEIIA
WWKSKGRDPRQKLLVFSDAMDVESIEQIHQRFSDQVRLSFGWGTNLTNDFVGCSPDGSVDLDPISLVCKVTSVDGRPAVK
LSDNPEKATGDPAEIARYLRVFGDAGRVRTPVFV

Sequences:

>Translated_434_residues
MTVTDIASRTYNHGWRLDPIVRSLLDTDFYKLLMLQMIREFYPDQKVTFSVINRTKRVRLGDVIDEGELRAQLDHARTIR
FSKKELIWLAGNTFYGKTHMFSPDFLAWLANFRLPEYELHKADGQYELHFHGLWTHTTMWEIPALAIVNELRSRQAMKGQ
GRFALDVLFARAKAKLWAKVERLRKLEGLRLSDFGTRRRHGFLWQRWCVEAVKEGLGSSFTGTSNVLLAMDNDLEAIGTN
AHELPMVAAALAKDDDELRFAPYRILDQWRQTYAGNLLIALPDAFGTKAFLRDAPDWVADWTGFRPDSAPPIQAGEEIIA
WWKSKGRDPRQKLLVFSDAMDVESIEQIHQRFSDQVRLSFGWGTNLTNDFVGCSPDGSVDLDPISLVCKVTSVDGRPAVK
LSDNPEKATGDPAEIARYLRVFGDAGRVRTPVFV
>Mature_433_residues
TVTDIASRTYNHGWRLDPIVRSLLDTDFYKLLMLQMIREFYPDQKVTFSVINRTKRVRLGDVIDEGELRAQLDHARTIRF
SKKELIWLAGNTFYGKTHMFSPDFLAWLANFRLPEYELHKADGQYELHFHGLWTHTTMWEIPALAIVNELRSRQAMKGQG
RFALDVLFARAKAKLWAKVERLRKLEGLRLSDFGTRRRHGFLWQRWCVEAVKEGLGSSFTGTSNVLLAMDNDLEAIGTNA
HELPMVAAALAKDDDELRFAPYRILDQWRQTYAGNLLIALPDAFGTKAFLRDAPDWVADWTGFRPDSAPPIQAGEEIIAW
WKSKGRDPRQKLLVFSDAMDVESIEQIHQRFSDQVRLSFGWGTNLTNDFVGCSPDGSVDLDPISLVCKVTSVDGRPAVKL
SDNPEKATGDPAEIARYLRVFGDAGRVRTPVFV

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family [H]

Homologues:

Organism=Escherichia coli, GI1787162, Length=420, Percent_Identity=33.0952380952381, Blast_Score=179, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324783, Length=435, Percent_Identity=29.1954022988506, Blast_Score=163, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638 [H]

Pfam domain/function: PF04095 NAPRTase [H]

EC number: =2.4.2.11 [H]

Molecular weight: Translated: 49506; Mature: 49375

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTDIASRTYNHGWRLDPIVRSLLDTDFYKLLMLQMIREFYPDQKVTFSVINRTKRVRL
CCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCEEE
GDVIDEGELRAQLDHARTIRFSKKELIWLAGNTFYGKTHMFSPDFLAWLANFRLPEYELH
CCCCCCCHHHHHHHHHHHEEECCCEEEEEECCEEECCCCCCCHHHHHHHHHCCCCCHHHC
KADGQYELHFHGLWTHTTMWEIPALAIVNELRSRQAMKGQGRFALDVLFARAKAKLWAKV
CCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERLRKLEGLRLSDFGTRRRHGFLWQRWCVEAVKEGLGSSFTGTSNVLLAMDNDLEAIGTN
HHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHCCC
AHELPMVAAALAKDDDELRFAPYRILDQWRQTYAGNLLIALPDAFGTKAFLRDAPDWVAD
CHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCHHHHH
WTGFRPDSAPPIQAGEEIIAWWKSKGRDPRQKLLVFSDAMDVESIEQIHQRFSDQVRLSF
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHEEEEECCCCHHHHHHHHHHHCCCEEEEE
GWGTNLTNDFVGCSPDGSVDLDPISLVCKVTSVDGRPAVKLSDNPEKATGDPAEIARYLR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHH
VFGDAGRVRTPVFV
HHCCCCCCCCCCCC
>Mature Secondary Structure 
TVTDIASRTYNHGWRLDPIVRSLLDTDFYKLLMLQMIREFYPDQKVTFSVINRTKRVRL
CCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCEEE
GDVIDEGELRAQLDHARTIRFSKKELIWLAGNTFYGKTHMFSPDFLAWLANFRLPEYELH
CCCCCCCHHHHHHHHHHHEEECCCEEEEEECCEEECCCCCCCHHHHHHHHHCCCCCHHHC
KADGQYELHFHGLWTHTTMWEIPALAIVNELRSRQAMKGQGRFALDVLFARAKAKLWAKV
CCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERLRKLEGLRLSDFGTRRRHGFLWQRWCVEAVKEGLGSSFTGTSNVLLAMDNDLEAIGTN
HHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHCCC
AHELPMVAAALAKDDDELRFAPYRILDQWRQTYAGNLLIALPDAFGTKAFLRDAPDWVAD
CHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCHHHHH
WTGFRPDSAPPIQAGEEIIAWWKSKGRDPRQKLLVFSDAMDVESIEQIHQRFSDQVRLSF
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHEEEEECCCCHHHHHHHHHHHCCCEEEEE
GWGTNLTNDFVGCSPDGSVDLDPISLVCKVTSVDGRPAVKLSDNPEKATGDPAEIARYLR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHH
VFGDAGRVRTPVFV
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA