Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 146337479

GI number: 146337479

Start: 335130

End: 336482

Strand: Reverse

Name: gatA [H]

Synonym: BRADO0324

Alternate gene names: 146337479

Gene position: 336482-335130 (Counterclockwise)

Preceding gene: 146337482

Following gene: 146337478

Centisome position: 4.51

GC content: 68.22

Gene sequence:

>1353_bases
ATGTCGCAACCGTCAGATCTTCAGACACCGAAAGCCGGTGCGGGCGTGCTCGAGCTGCTGCGCCAATTCGCCGCCGACCC
CGCATCCGCCTCCGCCTATGGCGAAGATTGTCTGAAGCGGACGCAGAGCATCGAGCCGCAGCTGAAGGCGTTCGAATATC
TGCCACGAGATGTGACCGCGAAGCGTGGTCCGCTCGGCGGAATTCCCGTGGCCATCAAGGACGTCATCGCGACCTCGGAC
ATGCCGACCACCAACGGGTCGGCGATCTATCGCGATCACGTGCCGGATGCCGACGCGTGGGTCGTGGCACGGCTGCGCGA
TCTCGGCGCGACCATCTTCGGCAAGACGGTGTCGACCGAATTCGCCTGGCGTCATCCCGGCCCGACCGTCAACCCTTGGA
ACCCCGCGCACACGCCCGGCGGCTCGTCGTCCGGCTCCGCCGCGGCCGTTGCTGCGGGACTCGTGCCGCTGGCGCTGGGC
ACGCAGACGCTGGGCTCGGTCATCCGTCCCGCGGCATTCAACGGCGTTGTCGGCTTCAAGCCGAGCTTCGGCGCGATTCC
GCGCACGGGCGTGCATCCGCTCAGCCCGTCGCTCGACCATGTCGGCTTCTTCGCGCGCCGTGTCGACGATGTGGCCCTTG
CTCTTTCTCTGCTCGCCGGCCGCAGCGCGGATGATCTCCACGGCCGTCCACTGCCCGGATTTTCAGTCGACATCGGGCAC
GGACTCGCGCCGCTCGCCAAGCCGCGCCTTGCGGTGGTGCGCTTCGCCAAATGGGAGCGCGCCGAGGCCGAGCAGAAGGC
AGTGTTCGAAGCTGTCATCGACAAGCTGCGTGGCGCAGGCGCGGCGATCGAGGAGGTCGCGCTGACGGAGCTGGACAGCG
CCAGCTGGGACGCGATCAACACGATCATGCTGAGCGAGGCGACGACGATCTTCTCCGACCTGATCGCGCGCTATCCGGAC
CGCGTCAGCGACGTGATGAAGGGCCACGTCGAGAGCGGACGAGCCAAGACGGCGATGGCGTACCTCGCCGCAAAGGCCAC
GCAGGCGGCCCGGCAGACGGCGCTCGCGGCAGAACTCGCGGGTCATGACGCGGTGCTCACCCTGCCGGCGTTCGGCGAGG
CGCCGCGCGGGCTCGACTGGACCGGCGATGCCGAATATTGCGCGCCCTGGACCTTCGTCGGCGCCCCGGCCGTTGCGTTG
CCAGCCGGCTTTGGCAGAAACGGCCTGCCGCTCGGCGTCCAGGTCGCAGGCACCTATCGCAACGATCTCCAGCTTCTCCG
CGTCGCGAAATGGGTGGAAACGGCGCTCGCCTTCGATCCCGGCCTGCCGCTCATGGCGCAACCGACGCGATGA

Upstream 100 bases:

>100_bases
TTGGCGCGGCCCTCATCCCCCGCTAGTCTGCGGCCCCACGAACGGCGTGAACGACGCCTCCAAGACACGCCTCCAAGACT
CGTGCCGAACAGGACAGCCG

Downstream 100 bases:

>100_bases
ACCGCTTGATGCCGGGGACCTCTCCGGTCACCGCAACAGAACCCTGCGCCAAAGCCCAGTGGCGGGTTTCCGGCCGGCGC
TGTAAAACCGTCGCATGACC

Product: putative amidase (amiD)

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSQPSDLQTPKAGAGVLELLRQFAADPASASAYGEDCLKRTQSIEPQLKAFEYLPRDVTAKRGPLGGIPVAIKDVIATSD
MPTTNGSAIYRDHVPDADAWVVARLRDLGATIFGKTVSTEFAWRHPGPTVNPWNPAHTPGGSSSGSAAAVAAGLVPLALG
TQTLGSVIRPAAFNGVVGFKPSFGAIPRTGVHPLSPSLDHVGFFARRVDDVALALSLLAGRSADDLHGRPLPGFSVDIGH
GLAPLAKPRLAVVRFAKWERAEAEQKAVFEAVIDKLRGAGAAIEEVALTELDSASWDAINTIMLSEATTIFSDLIARYPD
RVSDVMKGHVESGRAKTAMAYLAAKATQAARQTALAAELAGHDAVLTLPAFGEAPRGLDWTGDAEYCAPWTFVGAPAVAL
PAGFGRNGLPLGVQVAGTYRNDLQLLRVAKWVETALAFDPGLPLMAQPTR

Sequences:

>Translated_450_residues
MSQPSDLQTPKAGAGVLELLRQFAADPASASAYGEDCLKRTQSIEPQLKAFEYLPRDVTAKRGPLGGIPVAIKDVIATSD
MPTTNGSAIYRDHVPDADAWVVARLRDLGATIFGKTVSTEFAWRHPGPTVNPWNPAHTPGGSSSGSAAAVAAGLVPLALG
TQTLGSVIRPAAFNGVVGFKPSFGAIPRTGVHPLSPSLDHVGFFARRVDDVALALSLLAGRSADDLHGRPLPGFSVDIGH
GLAPLAKPRLAVVRFAKWERAEAEQKAVFEAVIDKLRGAGAAIEEVALTELDSASWDAINTIMLSEATTIFSDLIARYPD
RVSDVMKGHVESGRAKTAMAYLAAKATQAARQTALAAELAGHDAVLTLPAFGEAPRGLDWTGDAEYCAPWTFVGAPAVAL
PAGFGRNGLPLGVQVAGTYRNDLQLLRVAKWVETALAFDPGLPLMAQPTR
>Mature_449_residues
SQPSDLQTPKAGAGVLELLRQFAADPASASAYGEDCLKRTQSIEPQLKAFEYLPRDVTAKRGPLGGIPVAIKDVIATSDM
PTTNGSAIYRDHVPDADAWVVARLRDLGATIFGKTVSTEFAWRHPGPTVNPWNPAHTPGGSSSGSAAAVAAGLVPLALGT
QTLGSVIRPAAFNGVVGFKPSFGAIPRTGVHPLSPSLDHVGFFARRVDDVALALSLLAGRSADDLHGRPLPGFSVDIGHG
LAPLAKPRLAVVRFAKWERAEAEQKAVFEAVIDKLRGAGAAIEEVALTELDSASWDAINTIMLSEATTIFSDLIARYPDR
VSDVMKGHVESGRAKTAMAYLAAKATQAARQTALAAELAGHDAVLTLPAFGEAPRGLDWTGDAEYCAPWTFVGAPAVALP
AGFGRNGLPLGVQVAGTYRNDLQLLRVAKWVETALAFDPGLPLMAQPTR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=28.0487804878049, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI195972892, Length=444, Percent_Identity=24.3243243243243, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=427, Percent_Identity=26.6978922716628, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=405, Percent_Identity=26.6666666666667, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=259, Percent_Identity=28.957528957529, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=465, Percent_Identity=25.1612903225806, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=412, Percent_Identity=30.0970873786408, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=190, Percent_Identity=39.4736842105263, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=31.554524361949, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648441, Length=405, Percent_Identity=25.4320987654321, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648439, Length=405, Percent_Identity=25.4320987654321, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648435, Length=405, Percent_Identity=25.4320987654321, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648437, Length=405, Percent_Identity=25.4320987654321, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45550774, Length=405, Percent_Identity=25.4320987654321, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24652985, Length=436, Percent_Identity=25.4587155963303, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922090, Length=436, Percent_Identity=25.4587155963303, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652981, Length=436, Percent_Identity=25.4587155963303, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652983, Length=436, Percent_Identity=25.4587155963303, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161078093, Length=414, Percent_Identity=24.6376811594203, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47255; Mature: 47124

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPSDLQTPKAGAGVLELLRQFAADPASASAYGEDCLKRTQSIEPQLKAFEYLPRDVTA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHCC
KRGPLGGIPVAIKDVIATSDMPTTNGSAIYRDHVPDADAWVVARLRDLGATIFGKTVSTE
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHCCHHCCC
FAWRHPGPTVNPWNPAHTPGGSSSGSAAAVAAGLVPLALGTQTLGSVIRPAAFNGVVGFK
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSFGAIPRTGVHPLSPSLDHVGFFARRVDDVALALSLLAGRSADDLHGRPLPGFSVDIGH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
GLAPLAKPRLAVVRFAKWERAEAEQKAVFEAVIDKLRGAGAAIEEVALTELDSASWDAIN
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
TIMLSEATTIFSDLIARYPDRVSDVMKGHVESGRAKTAMAYLAAKATQAARQTALAAELA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHDAVLTLPAFGEAPRGLDWTGDAEYCAPWTFVGAPAVALPAGFGRNGLPLGVQVAGTYR
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCEEEEEECCCH
NDLQLLRVAKWVETALAFDPGLPLMAQPTR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQPSDLQTPKAGAGVLELLRQFAADPASASAYGEDCLKRTQSIEPQLKAFEYLPRDVTA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHCC
KRGPLGGIPVAIKDVIATSDMPTTNGSAIYRDHVPDADAWVVARLRDLGATIFGKTVSTE
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHCCHHCCC
FAWRHPGPTVNPWNPAHTPGGSSSGSAAAVAAGLVPLALGTQTLGSVIRPAAFNGVVGFK
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSFGAIPRTGVHPLSPSLDHVGFFARRVDDVALALSLLAGRSADDLHGRPLPGFSVDIGH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
GLAPLAKPRLAVVRFAKWERAEAEQKAVFEAVIDKLRGAGAAIEEVALTELDSASWDAIN
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
TIMLSEATTIFSDLIARYPDRVSDVMKGHVESGRAKTAMAYLAAKATQAARQTALAAELA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHDAVLTLPAFGEAPRGLDWTGDAEYCAPWTFVGAPAVALPAGFGRNGLPLGVQVAGTYR
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCEEEEEECCCH
NDLQLLRVAKWVETALAFDPGLPLMAQPTR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA