Click here to switch to the map view.

The map label for this gene is amtB2 [H]

Identifier: 146337462

GI number: 146337462

Start: 319040

End: 320542

Strand: Reverse

Name: amtB2 [H]

Synonym: BRADO0305

Alternate gene names: 146337462

Gene position: 320542-319040 (Counterclockwise)

Preceding gene: 146337463

Following gene: 146337458

Centisome position: 4.3

GC content: 66.67

Gene sequence:

>1503_bases
ATGACGTTCAAGCGTCCAACCAGCGCGGGATGGGTCACGCTCGCAATTGCCGGGCTCTGCATCGCGAGCCTCGCCGACGT
GGCGTTCGCTGAAGATGCGCCGGCCGCCGCTGCTGCAGCCGCTCCCGTTCCCAACAAGGGCGACACCGCCTGGATGCTCG
TGTCCAGCGCGCTCGTTCTGATGATGTCCGTCCCCGGCCTCGCGCTGTTCTATGGCGGCCTCGTCCGTACCAAGAACATG
GCCGCGATCCTGACTCAGGTGTTCGCGATCGTCGCGATGGTCGGCGTGGTCTGGACGCTCTACGGCTACTCGCTCGCCTT
CACCGATGGCGGCAGCATGACCTCGTGGATCGGCGGCTTCTCGAAGGCCTTCATGCACGGCATCGACGCCAACTCGACGG
CAGCGACGTTCTCGAACGGCGTCGTCATTCCGGAGCTCGCCTACTTCGTGTTCCAGATGACCTTCGCGATGATCACTCCG
GCCCTGATCGTCGGCGCCTTCGCCGAGCGCATCAAGTTCTCGGCGGTGATGCTGTTCGTGCTGCTCTGGGTCACCTTCAT
CTACTTCCCGATCGCGCACTGGGTCTGGTACATCGCCGCTCCGGACGACGTCGCTGCTGCTGCCAAGGCTCTCGCCGCCG
CTGGCGACGCCGCTGCCAAGACCGCCGCTCAGGCCAAGCTCGATGAAGTCACCGGCGCCGTCGGCTGGCTCGCCGGCGCT
GGCGCTCTCGACTTCGCCGGCGGCACCGTCGTTCACATCAACGCCGGCATCGCCGGCTTGGTGGGCGCTGTCCTGATCGG
CAAGCGCACCGGCTACGGCAAGGAGCTGATGGCCCCGCACTCGCTGACCATGACCATGATCGGCGCTTCGCTGCTGTGGG
TCGGCTGGTTCGGCTTCAATGCCGGTTCGAACCTCGAATCGAACGGCGTGACGGCTCTGGCCTTCGTCAACACCATGGTC
GCTACCGCCGGCGCGGCGCTGTCCTGGCTGCTGTGCGAATGGGCTGTCAAGGGCAAGCCCTCGCTGCTCGGCATCTGCTC
GGGCGCGGTGGCCGGTCTCGTCGCCGTCACCCCGGCCTCCGGCCTCGCCGGCCCGATCGGCGCGCTGGTCCTCGGCCTGA
TCGTCTCGCCGGTCTGCCTGTTCTTCGTCTCCACCGTGAAGAACTCGCTCGGCTATGACGACGCGCTGGACGTGTTCGGC
GTGCACTGCATCGGCGGCATCATCGGCGCGCTGGCCACCGGCATCCTGGTCAACCCGGCTCTCGGCGGCGTCGGCATCAC
CGACTACACCAACATCACCGGCAACAACGCCGGCACCTACGACTTCGCCACGCAGATGGTGGCGCAGATCAAGGCTGTCG
TCGCCACGCTGGTGTGGTCGGGTGTCGGCTCGGCGATCCTCTACAAGGTCGTCGACGTGATCATCGGCCTGCGTCCGACC
GTCGAGCAGGAGCGCGAGGGTCTGGACATCACCGACCACGGCGAGCGCGCCTACAACTACTGA

Upstream 100 bases:

>100_bases
CATCAGCCTCGACCACGCCGTGCGCATCCGCACCGGCGAAGCGGACGCTGCGGCTCTCTGATTTCGCGCTCAAACCAGAA
CACTTACGGAGTAGAATAAA

Downstream 100 bases:

>100_bases
GTTCTCCCGGGACTTGGTCCCACCGGACCAGGTCCGCAACTTCGGTTCGGGCAAATACCCGGCAATGCCCTGACCGTTGA
GGGGCTCCAGCGCAAGCTGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTFKRPTSAGWVTLAIAGLCIASLADVAFAEDAPAAAAAAAPVPNKGDTAWMLVSSALVLMMSVPGLALFYGGLVRTKNM
AAILTQVFAIVAMVGVVWTLYGYSLAFTDGGSMTSWIGGFSKAFMHGIDANSTAATFSNGVVIPELAYFVFQMTFAMITP
ALIVGAFAERIKFSAVMLFVLLWVTFIYFPIAHWVWYIAAPDDVAAAAKALAAAGDAAAKTAAQAKLDEVTGAVGWLAGA
GALDFAGGTVVHINAGIAGLVGAVLIGKRTGYGKELMAPHSLTMTMIGASLLWVGWFGFNAGSNLESNGVTALAFVNTMV
ATAGAALSWLLCEWAVKGKPSLLGICSGAVAGLVAVTPASGLAGPIGALVLGLIVSPVCLFFVSTVKNSLGYDDALDVFG
VHCIGGIIGALATGILVNPALGGVGITDYTNITGNNAGTYDFATQMVAQIKAVVATLVWSGVGSAILYKVVDVIIGLRPT
VEQEREGLDITDHGERAYNY

Sequences:

>Translated_500_residues
MTFKRPTSAGWVTLAIAGLCIASLADVAFAEDAPAAAAAAAPVPNKGDTAWMLVSSALVLMMSVPGLALFYGGLVRTKNM
AAILTQVFAIVAMVGVVWTLYGYSLAFTDGGSMTSWIGGFSKAFMHGIDANSTAATFSNGVVIPELAYFVFQMTFAMITP
ALIVGAFAERIKFSAVMLFVLLWVTFIYFPIAHWVWYIAAPDDVAAAAKALAAAGDAAAKTAAQAKLDEVTGAVGWLAGA
GALDFAGGTVVHINAGIAGLVGAVLIGKRTGYGKELMAPHSLTMTMIGASLLWVGWFGFNAGSNLESNGVTALAFVNTMV
ATAGAALSWLLCEWAVKGKPSLLGICSGAVAGLVAVTPASGLAGPIGALVLGLIVSPVCLFFVSTVKNSLGYDDALDVFG
VHCIGGIIGALATGILVNPALGGVGITDYTNITGNNAGTYDFATQMVAQIKAVVATLVWSGVGSAILYKVVDVIIGLRPT
VEQEREGLDITDHGERAYNY
>Mature_499_residues
TFKRPTSAGWVTLAIAGLCIASLADVAFAEDAPAAAAAAAPVPNKGDTAWMLVSSALVLMMSVPGLALFYGGLVRTKNMA
AILTQVFAIVAMVGVVWTLYGYSLAFTDGGSMTSWIGGFSKAFMHGIDANSTAATFSNGVVIPELAYFVFQMTFAMITPA
LIVGAFAERIKFSAVMLFVLLWVTFIYFPIAHWVWYIAAPDDVAAAAKALAAAGDAAAKTAAQAKLDEVTGAVGWLAGAG
ALDFAGGTVVHINAGIAGLVGAVLIGKRTGYGKELMAPHSLTMTMIGASLLWVGWFGFNAGSNLESNGVTALAFVNTMVA
TAGAALSWLLCEWAVKGKPSLLGICSGAVAGLVAVTPASGLAGPIGALVLGLIVSPVCLFFVSTVKNSLGYDDALDVFGV
HCIGGIIGALATGILVNPALGGVGITDYTNITGNNAGTYDFATQMVAQIKAVVATLVWSGVGSAILYKVVDVIIGLRPTV
EQEREGLDITDHGERAYNY

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=466, Percent_Identity=46.5665236051502, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17550182, Length=475, Percent_Identity=28, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=470, Percent_Identity=28.0851063829787, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=460, Percent_Identity=25.6521739130435, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI133931100, Length=475, Percent_Identity=23.7894736842105, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325396, Length=456, Percent_Identity=32.4561403508772, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321559, Length=461, Percent_Identity=30.58568329718, Blast_Score=200, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6324187, Length=470, Percent_Identity=31.2765957446809, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI161078314, Length=459, Percent_Identity=25.9259259259259, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51344; Mature: 51213

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFKRPTSAGWVTLAIAGLCIASLADVAFAEDAPAAAAAAAPVPNKGDTAWMLVSSALVL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHH
MMSVPGLALFYGGLVRTKNMAAILTQVFAIVAMVGVVWTLYGYSLAFTDGGSMTSWIGGF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
SKAFMHGIDANSTAATFSNGVVIPELAYFVFQMTFAMITPALIVGAFAERIKFSAVMLFV
HHHHHHCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLWVTFIYFPIAHWVWYIAAPDDVAAAAKALAAAGDAAAKTAAQAKLDEVTGAVGWLAGA
HHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
GALDFAGGTVVHINAGIAGLVGAVLIGKRTGYGKELMAPHSLTMTMIGASLLWVGWFGFN
CCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCC
AGSNLESNGVTALAFVNTMVATAGAALSWLLCEWAVKGKPSLLGICSGAVAGLVAVTPAS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GLAGPIGALVLGLIVSPVCLFFVSTVKNSLGYDDALDVFGVHCIGGIIGALATGILVNPA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGGVGITDYTNITGNNAGTYDFATQMVAQIKAVVATLVWSGVGSAILYKVVDVIIGLRPT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
VEQEREGLDITDHGERAYNY
HHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]