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The map label for this gene is hisF [H]

Identifier: 146337383

GI number: 146337383

Start: 227731

End: 228507

Strand: Direct

Name: hisF [H]

Synonym: BRADO0216

Alternate gene names: 146337383

Gene position: 227731-228507 (Clockwise)

Preceding gene: 146337382

Following gene: 146337384

Centisome position: 3.05

GC content: 66.8

Gene sequence:

>777_bases
ATGTTCAAGGTCCGCGTCATTCCCTGCCTCGACGTCAAGGACGGCCGTGTCGTCAAGGGCGTCAACTTCGTCAATCTGCG
CGACGCCGGCGATCCCGTCGAAGCCGCGATCGCCTATGACGCCGCCGGTGCCGACGAGCTCTGCTTTCTCGACATCACCG
CCACCCATGAGAACCGCGGCATCATGCTCGACGTCGTCCGGCGTACCGCGGAGGCCTGCTTCATGCCGGTCACCGTCGGC
GGCGGCGTGCGCACGATCGATGATATCAAGACATTGCTGCGCTCCGGCGCCGACAAGGTCTCGATCAACTCGGCTGCGGT
GAGCAGGCGCGAGTTCGTGAAGGAGGCGGCCGAGAAGTTCGGCGAGCAATGCATCGTGGTCGCGATCGACGCCAAGTCGG
TGCCGCGTCCGGGCGGCGGCTCGCGCTGGGAGATCTTCACTCATGGCGGCCGCAAATCGACGGGCATCGATGCGATTGAA
TATGCCCAGGAGGTCGTCGCGCTCGGCGCCGGCGAGATCCTGCTGACCTCGATGGACCGCGATGGCACGCGGCAGGGCTT
CGACCTGCCGCTGACCCGGGCGGTCGCCGACAGCGTCCCGGTGCCGGTGATCGCCTCCGGCGGCGTCGGCAATCTGGACC
ACCTGGTCGACGGCATCCAGCAGGGCAGGGCGACCGCCGTGCTGGCCGCCTCGATCTTCCATTTCGGGGAATTTACCATT
CGGCAGGCCAAGGAGCACATGGTGCGGCAGGGCCTGCCGATGCGGCTCGATCCCTGA

Upstream 100 bases:

>100_bases
TCGAAGGCGCCATCGCCGGCCGCGCGCTCTATGACGGGCGGCTGGATCCGGCGGAAGCGCTGGCGCTGATTCGCGCGATC
AGCGGGTAGGGGAGAACAAT

Downstream 100 bases:

>100_bases
CGACATTGCGCCGCAAAAATGTTAGATGAACGCGGGTCCGGCCGCCTTGGGAACGGACCTGCGTGTCCTGAGAACTGGCT
GATGTCCCGCTTCACGCTTC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MFKVRVIPCLDVKDGRVVKGVNFVNLRDAGDPVEAAIAYDAAGADELCFLDITATHENRGIMLDVVRRTAEACFMPVTVG
GGVRTIDDIKTLLRSGADKVSINSAAVSRREFVKEAAEKFGEQCIVVAIDAKSVPRPGGGSRWEIFTHGGRKSTGIDAIE
YAQEVVALGAGEILLTSMDRDGTRQGFDLPLTRAVADSVPVPVIASGGVGNLDHLVDGIQQGRATAVLAASIFHFGEFTI
RQAKEHMVRQGLPMRLDP

Sequences:

>Translated_258_residues
MFKVRVIPCLDVKDGRVVKGVNFVNLRDAGDPVEAAIAYDAAGADELCFLDITATHENRGIMLDVVRRTAEACFMPVTVG
GGVRTIDDIKTLLRSGADKVSINSAAVSRREFVKEAAEKFGEQCIVVAIDAKSVPRPGGGSRWEIFTHGGRKSTGIDAIE
YAQEVVALGAGEILLTSMDRDGTRQGFDLPLTRAVADSVPVPVIASGGVGNLDHLVDGIQQGRATAVLAASIFHFGEFTI
RQAKEHMVRQGLPMRLDP
>Mature_258_residues
MFKVRVIPCLDVKDGRVVKGVNFVNLRDAGDPVEAAIAYDAAGADELCFLDITATHENRGIMLDVVRRTAEACFMPVTVG
GGVRTIDDIKTLLRSGADKVSINSAAVSRREFVKEAAEKFGEQCIVVAIDAKSVPRPGGGSRWEIFTHGGRKSTGIDAIE
YAQEVVALGAGEILLTSMDRDGTRQGFDLPLTRAVADSVPVPVIASGGVGNLDHLVDGIQQGRATAVLAASIFHFGEFTI
RQAKEHMVRQGLPMRLDP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=39.6153846153846, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=28.9256198347107, Blast_Score=97, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=312, Percent_Identity=34.6153846153846, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27585; Mature: 27585

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVRVIPCLDVKDGRVVKGVNFVNLRDAGDPVEAAIAYDAAGADELCFLDITATHENRG
CEEEEEEEEECCCCCCEEECEEEEEECCCCCCHHEEEEECCCCCCCEEEEEEEEECCCCC
IMLDVVRRTAEACFMPVTVGGGVRTIDDIKTLLRSGADKVSINSAAVSRREFVKEAAEKF
CHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
GEQCIVVAIDAKSVPRPGGGSRWEIFTHGGRKSTGIDAIEYAQEVVALGAGEILLTSMDR
CCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTRQGFDLPLTRAVADSVPVPVIASGGVGNLDHLVDGIQQGRATAVLAASIFHFGEFTI
CCCCCCCCCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RQAKEHMVRQGLPMRLDP
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFKVRVIPCLDVKDGRVVKGVNFVNLRDAGDPVEAAIAYDAAGADELCFLDITATHENRG
CEEEEEEEEECCCCCCEEECEEEEEECCCCCCHHEEEEECCCCCCCEEEEEEEEECCCCC
IMLDVVRRTAEACFMPVTVGGGVRTIDDIKTLLRSGADKVSINSAAVSRREFVKEAAEKF
CHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
GEQCIVVAIDAKSVPRPGGGSRWEIFTHGGRKSTGIDAIEYAQEVVALGAGEILLTSMDR
CCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTRQGFDLPLTRAVADSVPVPVIASGGVGNLDHLVDGIQQGRATAVLAASIFHFGEFTI
CCCCCCCCCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RQAKEHMVRQGLPMRLDP
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA