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The map label for this gene is gidA [H]

Identifier: 146337362

GI number: 146337362

Start: 207993

End: 209867

Strand: Reverse

Name: gidA [H]

Synonym: BRADO0195

Alternate gene names: 146337362

Gene position: 209867-207993 (Counterclockwise)

Preceding gene: 146337363

Following gene: 146337361

Centisome position: 2.81

GC content: 66.08

Gene sequence:

>1875_bases
ATGACCGAACATACATCCTACGACGTCATCGTCATTGGCGGTGGCCACGCCGGCTGTGAGGCCGCGGCCGCCTCCGCCCG
CATGGGCGCGCGCACTGCCCTGGTCACGCATCGGTTCGCGACCATCGGCGCGATGTCCTGCAATCCTGCCATAGGCGGCT
TGGGCAAGGGGCATCTGGTCCGCGAGGTCGACGCGCTCGACGGTCTGATGGGGCGGGTCGCAGACGCCGGCGGTATCCAG
TTTCGGGTTCTGAACCGGCGGAAGGGACCGGCCGTCCGCGGTCCGCGCGCCCAGGCGGATCGCAAGCTCTATGCCGCCGC
GATGCAGTCCGCGATCCGCGAGACCACCAACCTTGCGGTGGTCGAAGGAGAAGCGGATGACCTGATCGTTGTCGATGGTC
GCGTGACAGGGATCAAACTGGCTGACGGCCGATCCCTGGCCTGTGGGGCGGTGGTGATCACCACCGGCACATTCCTGCGC
GGCCTGATCCATCTCGGGGAGAAGACCTGGCCGGCCGGCCGGGTCGGCGAAGCGCCGGCGCTCGGCTTGTCGCGCTCGCT
TGAAGCCATCGGCTTCAATCTGGGTCGTCTCAAGACCGGGACGCCGCCACGGCTCGACGGCACCACGATCGATTGGGGTG
CGGTCGAGATGCAGACGGGTGACGATCCGGCCGAGCCATTCTCCGTGCTGACCGAGACGATCACGACGCCGCAGATCCAG
TGCGGTATCACCCGGACGACGATCGCGACCCACGAGGTGATCCGCGCCAACGTCCATCGCTCGCCGATGTATTCCGGCCA
GATCAAGTCGACCGGCCCGCGCTATTGCCCGTCCGTTGAGGACAAGATCGTCCGTTTCGGCGACCGCGACGGGCACCAGA
TCTTCCTCGAGCCGGAAGGGCTCGACGACTCCACGGTTTATCCCAACGGCATCTCCACCTCATTGCCGGAAGAGGTTCAG
CTCGCGCTGCTTCCGACCATTCCAGGATTGGAGCGGGTCCGCATGATCCGTCCGGGCTATGCCATCGAATATGATCACGT
CGATCCGCGCGAGCTCGAGCCGACCCTGCAAACCAAGCGGATGAAAGGTCTGTTTCTGGCCGGCCAGATCAACGGCACCA
CGGGATACGAGGAGGCCGCAGCCCAGGGTCTGGTGGCAGGTATCAACGCCGCGCTCGCCGCCGGAGGCGGAGCGCCGATC
GTGTTCGATCGCGCCGACGGCTATCTCGGCGTCATGATTGACGACCTGGTGACCCGAGGCATCACCGAGCCGTATCGGAT
GTTCACCTCGCGCGCCGAGTATCGCCTGACCCTTCGCGCCGACAATGCCGATCAGCGGCTGACCGACAAGGGCATCGCGC
TCGGCTGTGTCGGAGGTGAGCGGGTGCGTTTCCACCGACGCAAGCTGGCGGCGCTGCAGGCGGCGCGAGCTGTCGCGACG
TCGCTGACGATCACGCCGAACGAGGCCGCGAAGCACGGGCTTACGCTCAACAGGGATGGCCAGCGCCGCTCGGCGTTCGA
GCTGATGGCCTATCCCGAGATCGACTTCGCGAAGGTGCGGTCGATCTGGCCCGAGCTCGAAGCGATCGATCCGGTCGTAG
CCACCCATCTCGAGATCGATGCCAAATATGACGTCTACGTCCGCCGGCAGAGCGAGGACGTCGACGCCTTCCGGCGCGAC
GAGGGCTTGGTGCTGGGCGACATTGACTATGCGATCGTCCCAGGTCTGTCGAACGAGGCGCGCGCCAAGCTGACCGCGGC
GAAGCCGTGGACGATCGGGCAGGCCGGTCGGATCGATGGGATGACGCCGGCGGCGCTTGGAATTCTTGCGGCGTATCTTC
GCCGCGAAGCTCGGCGCAACAAGGCTGCGAGCTGA

Upstream 100 bases:

>100_bases
GCGATGTGCGCTGTTTCACGTGAAACATGGCGACGTGCCGAACTCCTTTTTCACGTGAAACGTCTTCCGGGCGTCGTCGC
CAATCGCTAGATAAGCTGCC

Downstream 100 bases:

>100_bases
GGAACGTTTCACGTGAAACCGACATCGTCCGCAGCGGCACGGAATGAGCTCGCATCTGACAAGGCGGCGGCTCTGGCGCT
GACTCCCGTTTCACGTGAAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MTEHTSYDVIVIGGGHAGCEAAAASARMGARTALVTHRFATIGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQ
FRVLNRRKGPAVRGPRAQADRKLYAAAMQSAIRETTNLAVVEGEADDLIVVDGRVTGIKLADGRSLACGAVVITTGTFLR
GLIHLGEKTWPAGRVGEAPALGLSRSLEAIGFNLGRLKTGTPPRLDGTTIDWGAVEMQTGDDPAEPFSVLTETITTPQIQ
CGITRTTIATHEVIRANVHRSPMYSGQIKSTGPRYCPSVEDKIVRFGDRDGHQIFLEPEGLDDSTVYPNGISTSLPEEVQ
LALLPTIPGLERVRMIRPGYAIEYDHVDPRELEPTLQTKRMKGLFLAGQINGTTGYEEAAAQGLVAGINAALAAGGGAPI
VFDRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDKGIALGCVGGERVRFHRRKLAALQAARAVAT
SLTITPNEAAKHGLTLNRDGQRRSAFELMAYPEIDFAKVRSIWPELEAIDPVVATHLEIDAKYDVYVRRQSEDVDAFRRD
EGLVLGDIDYAIVPGLSNEARAKLTAAKPWTIGQAGRIDGMTPAALGILAAYLRREARRNKAAS

Sequences:

>Translated_624_residues
MTEHTSYDVIVIGGGHAGCEAAAASARMGARTALVTHRFATIGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQ
FRVLNRRKGPAVRGPRAQADRKLYAAAMQSAIRETTNLAVVEGEADDLIVVDGRVTGIKLADGRSLACGAVVITTGTFLR
GLIHLGEKTWPAGRVGEAPALGLSRSLEAIGFNLGRLKTGTPPRLDGTTIDWGAVEMQTGDDPAEPFSVLTETITTPQIQ
CGITRTTIATHEVIRANVHRSPMYSGQIKSTGPRYCPSVEDKIVRFGDRDGHQIFLEPEGLDDSTVYPNGISTSLPEEVQ
LALLPTIPGLERVRMIRPGYAIEYDHVDPRELEPTLQTKRMKGLFLAGQINGTTGYEEAAAQGLVAGINAALAAGGGAPI
VFDRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDKGIALGCVGGERVRFHRRKLAALQAARAVAT
SLTITPNEAAKHGLTLNRDGQRRSAFELMAYPEIDFAKVRSIWPELEAIDPVVATHLEIDAKYDVYVRRQSEDVDAFRRD
EGLVLGDIDYAIVPGLSNEARAKLTAAKPWTIGQAGRIDGMTPAALGILAAYLRREARRNKAAS
>Mature_623_residues
TEHTSYDVIVIGGGHAGCEAAAASARMGARTALVTHRFATIGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQF
RVLNRRKGPAVRGPRAQADRKLYAAAMQSAIRETTNLAVVEGEADDLIVVDGRVTGIKLADGRSLACGAVVITTGTFLRG
LIHLGEKTWPAGRVGEAPALGLSRSLEAIGFNLGRLKTGTPPRLDGTTIDWGAVEMQTGDDPAEPFSVLTETITTPQIQC
GITRTTIATHEVIRANVHRSPMYSGQIKSTGPRYCPSVEDKIVRFGDRDGHQIFLEPEGLDDSTVYPNGISTSLPEEVQL
ALLPTIPGLERVRMIRPGYAIEYDHVDPRELEPTLQTKRMKGLFLAGQINGTTGYEEAAAQGLVAGINAALAAGGGAPIV
FDRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDKGIALGCVGGERVRFHRRKLAALQAARAVATS
LTITPNEAAKHGLTLNRDGQRRSAFELMAYPEIDFAKVRSIWPELEAIDPVVATHLEIDAKYDVYVRRQSEDVDAFRRDE
GLVLGDIDYAIVPGLSNEARAKLTAAKPWTIGQAGRIDGMTPAALGILAAYLRREARRNKAAS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=642, Percent_Identity=45.7943925233645, Blast_Score=541, Evalue=1e-154,
Organism=Homo sapiens, GI19882217, Length=667, Percent_Identity=44.0779610194903, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI183227703, Length=682, Percent_Identity=43.108504398827, Blast_Score=523, Evalue=1e-148,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=50.7154213036566, Blast_Score=604, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=43.7299035369775, Blast_Score=490, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6321202, Length=635, Percent_Identity=46.9291338582677, Blast_Score=569, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24658174, Length=629, Percent_Identity=47.8537360890302, Blast_Score=543, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 67003; Mature: 66872

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTSYDVIVIGGGHAGCEAAAASARMGARTALVTHRFATIGAMSCNPAIGGLGKGHLV
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCHH
REVDALDGLMGRVADAGGIQFRVLNRRKGPAVRGPRAQADRKLYAAAMQSAIRETTNLAV
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEE
VEGEADDLIVVDGRVTGIKLADGRSLACGAVVITTGTFLRGLIHLGEKTWPAGRVGEAPA
EECCCCCEEEECCEEEEEEEECCCCEECCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCC
LGLSRSLEAIGFNLGRLKTGTPPRLDGTTIDWGAVEMQTGDDPAEPFSVLTETITTPQIQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCHHHHHHHHHCCCCEEE
CGITRTTIATHEVIRANVHRSPMYSGQIKSTGPRYCPSVEDKIVRFGDRDGHQIFLEPEG
ECCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCC
LDDSTVYPNGISTSLPEEVQLALLPTIPGLERVRMIRPGYAIEYDHVDPRELEPTLQTKR
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHEEECCCCEEEECCCCHHHCCCHHHHHH
MKGLFLAGQINGTTGYEEAAAQGLVAGINAALAAGGGAPIVFDRADGYLGVMIDDLVTRG
HCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHC
ITEPYRMFTSRAEYRLTLRADNADQRLTDKGIALGCVGGERVRFHRRKLAALQAARAVAT
CCHHHHHHHCCCEEEEEEEECCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
SLTITPNEAAKHGLTLNRDGQRRSAFELMAYPEIDFAKVRSIWPELEAIDPVVATHLEID
EEEECCCHHHHCCCEECCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHCCCHHEEEEEEC
AKYDVYVRRQSEDVDAFRRDEGLVLGDIDYAIVPGLSNEARAKLTAAKPWTIGQAGRIDG
CEEEEEEEECCCCHHHHHCCCCEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
MTPAALGILAAYLRREARRNKAAS
CCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TEHTSYDVIVIGGGHAGCEAAAASARMGARTALVTHRFATIGAMSCNPAIGGLGKGHLV
CCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCHH
REVDALDGLMGRVADAGGIQFRVLNRRKGPAVRGPRAQADRKLYAAAMQSAIRETTNLAV
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEE
VEGEADDLIVVDGRVTGIKLADGRSLACGAVVITTGTFLRGLIHLGEKTWPAGRVGEAPA
EECCCCCEEEECCEEEEEEEECCCCEECCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCC
LGLSRSLEAIGFNLGRLKTGTPPRLDGTTIDWGAVEMQTGDDPAEPFSVLTETITTPQIQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCHHHHHHHHHCCCCEEE
CGITRTTIATHEVIRANVHRSPMYSGQIKSTGPRYCPSVEDKIVRFGDRDGHQIFLEPEG
ECCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCC
LDDSTVYPNGISTSLPEEVQLALLPTIPGLERVRMIRPGYAIEYDHVDPRELEPTLQTKR
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHEEECCCCEEEECCCCHHHCCCHHHHHH
MKGLFLAGQINGTTGYEEAAAQGLVAGINAALAAGGGAPIVFDRADGYLGVMIDDLVTRG
HCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHC
ITEPYRMFTSRAEYRLTLRADNADQRLTDKGIALGCVGGERVRFHRRKLAALQAARAVAT
CCHHHHHHHCCCEEEEEEEECCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
SLTITPNEAAKHGLTLNRDGQRRSAFELMAYPEIDFAKVRSIWPELEAIDPVVATHLEID
EEEECCCHHHHCCCEECCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHCCCHHEEEEEEC
AKYDVYVRRQSEDVDAFRRDEGLVLGDIDYAIVPGLSNEARAKLTAAKPWTIGQAGRIDG
CEEEEEEEECCCCHHHHHCCCCEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
MTPAALGILAAYLRREARRNKAAS
CCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA