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The map label for this gene is trmE

Identifier: 146337363

GI number: 146337363

Start: 210093

End: 211427

Strand: Reverse

Name: trmE

Synonym: BRADO0196

Alternate gene names: 146337363

Gene position: 211427-210093 (Counterclockwise)

Preceding gene: 146337364

Following gene: 146337362

Centisome position: 2.84

GC content: 65.24

Gene sequence:

>1335_bases
ATGCATCCTCACGACCAGACCATCTTCGCACTGTCCTCCGGGCGACTGCCGAGCGCGATCGCCATCGTCCGGCTGTCGGG
TCCACATGCAGGCGCCGCTCTTCAGATGTTGGCCGGGAAGATGCCGGCGCCGCGCCTGGTAACACGCGCGTTGTTGCGCG
ATCCCCAGGGCGAGCCGATCGACGATGCCGTGGTGCTGTGGTTTCCAGCCCCGGCTAGCGCCACCGGCGAGAACGTCGTC
GAGCTCCACATCCATGGTAGCCGCGCGGTCATCGCGACGTTGTTCGGGGTGTTGTCCGAGACGCCCGAGCTACGTCCAGC
AGAGCCGGGCGAGTTTACGCGGCGTGCGTTCGAGAACGGCAAGCTCGATCTGACCGAGGCCGAAGGTCTCGACGATCTCA
TTCATGCCGACACGGATCGCCAGCGGCGCCAGGCGCTGCGGCAGTTGAAAGGTTTGCTCGGTGACAAAGTGCGAAGATGG
CGCGAGCAGATCATCGAAGCCGCGGCCCTGATCGAGGCCGGAATCGACTTCTCCGATGAAGGCGACGTGCCCGCGGAGCT
GTTGGCGCCGGCGCTGTCGCGAGTTCAACAGCTCCTCTTAGAGATCGAAGCCGTGCTGGCAGCGCAGGGGCGGGCCGAGC
GGCTCCGTGACGGTCTCACCGTCGTCATTGCTGGACCACCCAATGCTGGCAAATCGACCTTGATGAACCAGCTGGCGCGG
CGCGAGGTGGCGATCGTCTCGCCCCAGGCCGGCACGACGCGGGATCTTATCGAAGTCCAGCTCGACCTCGACGGCTATCC
GGTGACGGTGATCGACACCGCGGGCATCCGGGAGACCGACGATCCGGTCGAGCAGGAGGGTGTCCGGCGGGCGCGGGACC
GCGCGGCTCACGCTGACCTGGTTCTTTGGTTGTCGGAGCACGACGGCGCCGAGGTGGAACGGCAAGGTGAAACGCCGCTC
TGGCTGGTTCGGACGAAGACAGATCTGGACGGCGCAGAACGGGCCACACGGCCCGATACAAAGGCGAGCCGGACATTTGG
GATCTCCGCAAGGAGCGGAGCAGGGATGGGGGCGCTGCTGGATGCTCTCGTGGGCTTCGCCCGCAACTTCTTTGGTTCGA
CCGAGAGCGGACTCATCACCCGGGAGCGTCAGAGACAATCCCTTCGGCAGACGGCTGATGCGCTTCAGCGAAGTCTGGAC
ACGGTCGATCTTGGCGAGGAACTCGTCGCCGAGGAACTGAGGATCGCCGCGTTGGCCCTCGGCCGGCTGCTGGGGCGGGT
CGATGTCGAGGATCTGCTCGATGTGATCTTCCGAGAGTTCTGCATCGGAAAGTAG

Upstream 100 bases:

>100_bases
GGATCAGGGTCTCGGGTTGCCGTGCGGCATGGCCATTCGGCGTAAAGCATGACCCCGCAAACCGATTGTCTTTCCGGCCC
ACTGCGCCCAACTGAGGCTC

Downstream 100 bases:

>100_bases
ATAGCGGAGACGCCATCGGCTCCGATATCCTTAAGCAAAGGTTGTTTGGTTAACCGATCGTCAAACGTTTCACGTGAAAC
AGGCATACGTCTCTCGGAGT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MHPHDQTIFALSSGRLPSAIAIVRLSGPHAGAALQMLAGKMPAPRLVTRALLRDPQGEPIDDAVVLWFPAPASATGENVV
ELHIHGSRAVIATLFGVLSETPELRPAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTDRQRRQALRQLKGLLGDKVRRW
REQIIEAAALIEAGIDFSDEGDVPAELLAPALSRVQQLLLEIEAVLAAQGRAERLRDGLTVVIAGPPNAGKSTLMNQLAR
REVAIVSPQAGTTRDLIEVQLDLDGYPVTVIDTAGIRETDDPVEQEGVRRARDRAAHADLVLWLSEHDGAEVERQGETPL
WLVRTKTDLDGAERATRPDTKASRTFGISARSGAGMGALLDALVGFARNFFGSTESGLITRERQRQSLRQTADALQRSLD
TVDLGEELVAEELRIAALALGRLLGRVDVEDLLDVIFREFCIGK

Sequences:

>Translated_444_residues
MHPHDQTIFALSSGRLPSAIAIVRLSGPHAGAALQMLAGKMPAPRLVTRALLRDPQGEPIDDAVVLWFPAPASATGENVV
ELHIHGSRAVIATLFGVLSETPELRPAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTDRQRRQALRQLKGLLGDKVRRW
REQIIEAAALIEAGIDFSDEGDVPAELLAPALSRVQQLLLEIEAVLAAQGRAERLRDGLTVVIAGPPNAGKSTLMNQLAR
REVAIVSPQAGTTRDLIEVQLDLDGYPVTVIDTAGIRETDDPVEQEGVRRARDRAAHADLVLWLSEHDGAEVERQGETPL
WLVRTKTDLDGAERATRPDTKASRTFGISARSGAGMGALLDALVGFARNFFGSTESGLITRERQRQSLRQTADALQRSLD
TVDLGEELVAEELRIAALALGRLLGRVDVEDLLDVIFREFCIGK
>Mature_444_residues
MHPHDQTIFALSSGRLPSAIAIVRLSGPHAGAALQMLAGKMPAPRLVTRALLRDPQGEPIDDAVVLWFPAPASATGENVV
ELHIHGSRAVIATLFGVLSETPELRPAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTDRQRRQALRQLKGLLGDKVRRW
REQIIEAAALIEAGIDFSDEGDVPAELLAPALSRVQQLLLEIEAVLAAQGRAERLRDGLTVVIAGPPNAGKSTLMNQLAR
REVAIVSPQAGTTRDLIEVQLDLDGYPVTVIDTAGIRETDDPVEQEGVRRARDRAAHADLVLWLSEHDGAEVERQGETPL
WLVRTKTDLDGAERATRPDTKASRTFGISARSGAGMGALLDALVGFARNFFGSTESGLITRERQRQSLRQTADALQRSLD
TVDLGEELVAEELRIAALALGRLLGRVDVEDLLDVIFREFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=461, Percent_Identity=45.5531453362256, Blast_Score=341, Evalue=9e-94,
Organism=Homo sapiens, GI193082993, Length=443, Percent_Identity=46.27539503386, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI193082988, Length=493, Percent_Identity=42.7991886409736, Blast_Score=326, Evalue=3e-89,
Organism=Escherichia coli, GI2367268, Length=465, Percent_Identity=37.4193548387097, Blast_Score=257, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17507259, Length=457, Percent_Identity=33.6980306345733, Blast_Score=225, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=31.8458417849899, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=38.4937238493724, Blast_Score=295, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BRASO (A4YJT5)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202411.1
- STRING:   A4YJT5
- GeneID:   5120979
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0196
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- BioCyc:   BSP376:BRADO0196-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48237; Mature: 48237

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 81-81 BINDING 121-121 BINDING 444-444

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPHDQTIFALSSGRLPSAIAIVRLSGPHAGAALQMLAGKMPAPRLVTRALLRDPQGEPI
CCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
DDAVVLWFPAPASATGENVVELHIHGSRAVIATLFGVLSETPELRPAEPGEFTRRAFENG
CCEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
KLDLTEAEGLDDLIHADTDRQRRQALRQLKGLLGDKVRRWREQIIEAAALIEAGIDFSDE
CEEEEHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDVPAELLAPALSRVQQLLLEIEAVLAAQGRAERLRDGLTVVIAGPPNAGKSTLMNQLAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
REVAIVSPQAGTTRDLIEVQLDLDGYPVTVIDTAGIRETDDPVEQEGVRRARDRAAHADL
CCEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEE
VLWLSEHDGAEVERQGETPLWLVRTKTDLDGAERATRPDTKASRTFGISARSGAGMGALL
EEEEECCCCCCHHCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCEECCCCCCCCCHHHHH
DALVGFARNFFGSTESGLITRERQRQSLRQTADALQRSLDTVDLGEELVAEELRIAALAL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHH
GRLLGRVDVEDLLDVIFREFCIGK
HHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHPHDQTIFALSSGRLPSAIAIVRLSGPHAGAALQMLAGKMPAPRLVTRALLRDPQGEPI
CCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
DDAVVLWFPAPASATGENVVELHIHGSRAVIATLFGVLSETPELRPAEPGEFTRRAFENG
CCEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
KLDLTEAEGLDDLIHADTDRQRRQALRQLKGLLGDKVRRWREQIIEAAALIEAGIDFSDE
CEEEEHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDVPAELLAPALSRVQQLLLEIEAVLAAQGRAERLRDGLTVVIAGPPNAGKSTLMNQLAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
REVAIVSPQAGTTRDLIEVQLDLDGYPVTVIDTAGIRETDDPVEQEGVRRARDRAAHADL
CCEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEE
VLWLSEHDGAEVERQGETPLWLVRTKTDLDGAERATRPDTKASRTFGISARSGAGMGALL
EEEEECCCCCCHHCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCEECCCCCCCCCHHHHH
DALVGFARNFFGSTESGLITRERQRQSLRQTADALQRSLDTVDLGEELVAEELRIAALAL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHH
GRLLGRVDVEDLLDVIFREFCIGK
HHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA